Analysis of cleft palate pathogenesis caused by post-fusional rupture

• Project/Area Number: 20592332
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Okayama University
• Principal Investigator: YAMADA Tomohiro Okayama University, 教育研究部・医療学系, 准教授 (60335619)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Katsuaki 山口大学, 大学院・医学系研究科, 准教授 (60304317) ; HIRATA Azumi 大阪大学, 大学院・工学研究科, 特任研究員 (40263587) ; MATSUMURA Tatsushi 岡山大学, 病院, 助教 (70432648) ; MORITANI Norifumi 岡山大学, 病院, 助教 (60467751)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 口蓋裂 / マウス / ダイオキシン / 解離 / 接着因子

Research Abstract

Mice treated with TCDD (2, 3, 7, 8-tetrachrolodibenzo dioxin) develop cleft palate following disruption, regardless the palate once fused. Factors related to the disruption of the palate, were considered for the lack of physical strength of the tissues. Therefore, intercellular adhesion molecule and basement membrane components were analyzed in this study. As a result, TCDD decreased expression of these molecules. Furthermore, morphologically bony hypoplasia was observed in the palatal bone of TCDD-treated group.
In conclusion, TCDD pathogenesis of cleft palate in mice was suggested to be caused by not only insufficient strength of palatal epithelium, but also hypoplasia of the muscles and bone.

Research Products

• [Journal Article] Effect of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin suggests abnormal palate development after palatal fusion. (2010)
  • Author(s): Imura H, Yamada T, Mishima K, Fujiwara K, Kawaki H, Hirata A, Sogawa N, Ueno T, Sugahara T.
  • Journal Title: Congenital Anomalies 50 Pages: 77-84
  • NAID: 10029577625
  • Peer Reviewed
• [Journal Article] Lyapnov exponents for Japanese vowels in normal adult and cleft palate speakers. (2010)
  • Author(s): 三島克章, 山田朋弘, 松村達志, 森谷徳文
  • Journal Title: Folia Phoniatr Logop 63 Pages: 129-133
  • Peer Reviewed
• [Journal Article] Nasolabial morphological changes after a Le Fort I osteotomy : a three-dimensional anthropometric study. (2010)
  • Author(s): Yamada T, Mishima K, Moritani N, Janune D, Matsumura T, Ikeya Y, Yamamoto T.
  • Journal Title: Journal of Craniofacial Surgery 21 Pages: 1089-1095
  • Peer Reviewed
• [Journal Article] Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate. (2010)
  • Author(s): Shirasu N, Ueno T, Hirata A, Kagawa T, Kanou M, Sawaki M, Wakimoto M, Ota A, Moritani N, Imura H, Matsumura T, Yamada T., Yamachika E.
  • Journal Title: Acta Histochemica 112 Pages: 270-277
  • Peer Reviewed
• [Journal Article] A case of ATLL (adult T-cell leukemia/lymphoma) mimicking dental infectious disease. (2010)
  • Author(s): Yamada T, Mishima K, Ota A, Moritani N, Matsumura T, Katase N, Yamamoto T.
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109
  • Peer Reviewed
• [Journal Article] Effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin suggests abnormal palate development after palatal fusion (2010)
  • Author(s): Imura H, Yamada T, Mishima K, Fujiwara K, Kawaki H, Hirata A, Sogawa N, Ueno T, Sugahara T
  • Journal Title: Congenital Anomalies Volume: 50 Pages: 77-84
  • NAID: 10029577625
  • Peer Reviewed
• [Journal Article] Lyapnov exponents for Japanese vowels in normal adult and cleft palate speakers (2010)
  • Author(s): Mishima K, Yamada T, Matsumura T, Moritani N
  • Journal Title: Folia Phoniatr Logop Volume: 63 Pages: 129-133
  • Peer Reviewed
• [Journal Article] Nasolabial morphological changes after a Le Fort I osteotomy : a three-dimensional anthropometric study (2010)
  • Author(s): Yamada T, Mishima K, Moritani N, Janune D, Matsumura T, Ikeya Y, Yamamoto T
  • Journal Title: Journal of Craniofacial Surgery Volume: 21 Pages: 1089-1095
  • Peer Reviewed
• [Journal Article] Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate (2010)
  • Author(s): Shirasu N, Ueno T, Hirata A, Kagawa T, Kanou M, Sawaki M, Wakimoto M, Ota A, Moritani N, Imura H, Matsumura T, Yamada T, Yamachika E
  • Journal Title: Acta Histochemica Volume: 112 Pages: 270-277
  • Peer Reviewed
• [Journal Article] A case of ATLL (adult T-cell leukemia/lymphoma) mimicking dental infectious disease (2010)
  • Author(s): Yamada T, Mishima K, Ota A, Moritani N, Matsumura T, Katase N, Yamamoto T
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Volume: 109
  • Peer Reviewed
• [Journal Article] Bone formation in a rat calvarial defect model after transplanting autogenous bone marrow with beta-tricalcium phosphate (2010)
  • Author(s): Shirasu N, Ueno T, Hirata A, Kagawa T, Kanou M, Sawaki M, Wakimoto M, Ota A, Moritani N, Imura H, Matsumura T, Yamada T, Yamachika E
  • Journal Title: Acta Histochemica 112 Pages: 270-277
  • Peer Reviewed
• [Journal Article] Primary intraosseous squamous cell carcinomas : five new clinicopathologic case studies. (2009)
  • Author(s): Yamada T, Ueno T, Moritani N, Mishima K, Hirata A, Matsumura T.
  • Journal Title: J Cranio-Maxillofacial Surgery 37 Pages: 448-453
  • Peer Reviewed
• [Journal Article] Osteomyelitis of the mandible secondary to infantile osteopetrosis : a case report. (2009)
  • Author(s): Yamada T, Mishima K, Imura H, Ueno T, Matsumura T, Moritani N
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107
  • Peer Reviewed
• [Journal Article] Postoperative course after SSRO in mandibular asymmetries with or without MMF. (2009)
  • Author(s): Yamada T, Mishima K, Matsumura T, Moritani N, Janune D, Sugahara T.
  • Journal Title: Oral and Maxillofacial Surgery 13 Pages: 27-31
  • Peer Reviewed
• [Journal Article] 先天性心疾患を合併した口唇口蓋裂患者の治療. (2009)
  • Author(s): 山田朋弘、三島克章、森谷徳文、松村達志、Danilo Janune.
  • Journal Title: 岡山大学歯学雑誌 28 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Primary intraosseous squamous cell carcinomas : five new clinicopathologic case studies (2009)
  • Author(s): Yamada T, Ueno T, Moritani N, Mishima K, Hirata A, Matsumura T
  • Journal Title: J Cranio-Maxillofacial Surgery 37 Pages: 448-453
  • Peer Reviewed
• [Journal Article] Osteomyelitis of the mandible secondary to infantile osteopetrosis : a case report (2009)
  • Author(s): Yamada T, Mishima K, Imura H, Ueno T, Matsumura T, Moritani N
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107
  • Peer Reviewed
• [Journal Article] Postoperative course after SSRO in mandibular asymmetries with or without MMF (2009)
  • Author(s): Yamada T, Mishima K, Matsumura T, Moritani N, Janune D, Sugahara T
  • Journal Title: Oral and Maxillofacial Surgery 13 Pages: 27-31
  • Peer Reviewed
• [Journal Article] 先天性心疾患を合併した口唇口蓋裂患者の治療 (2009)
  • Author(s): 山田朋弘, 三島克章, 林谷徳文, 松村達志, Danilo Janune
  • Journal Title: 岡山大学歯学雑誌 28 Pages: 19-25
  • Peer Reviewed
• [Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin in mice. (2008)
  • Author(s): Fujiwara K, Yamada T, Mishima K, Imura H, Sugahara T.
  • Journal Title: Congenital Anomalies 48 Pages: 68-73
  • NAID: 10027387216
  • Peer Reviewed
• [Journal Article] Mandibular stability after SSRO with or without post-operative maxillo-mandibular fixation forclass III malocclusion. (2008)
  • Author(s): Yamada T, Mishima K, Matsumura T, Moritani N, Sugahara T.
  • Journal Title: Oral and Maxillofacial Surgery 12 Pages: 177-180
  • Peer Reviewed
• [Journal Article] Morphological and immunohistochemical study on cleft palate induced by 2, 3, 7, 8 - Tetrachlorodibenzo - p - dioxin in mice (2008)
  • Author(s): Fujiwara, K., Yamada, T., Mishima, K., Imura, H., Sugahara, T.
  • Journal Title: Congenital Anomalies 48 Pages: 68-73
  • NAID: 10027387216
  • Peer Reviewed
• [Journal Article] Mandibular stability after SSRO with or without post-operative maxillo-mandibular fixat ion forclass III malocclusion. (2008)
  • Author(s): T. Yamada, K. Mishima, A., T. Matsumura, N. Moritani, T. Sugahara.
  • Journal Title: Oral and Maxillofacial Surgery 12 Pages: 177-180
  • Peer Reviewed
• [Presentation] 口唇口蓋裂に関する実験的研究-第124報-器官培養による口蓋突起癒合時の観察 (2010)
  • Author(s): 井村英人, 平田あずみ, 山田朋弘, 南克浩, 新美照幸, 古川博雄, 三島克章, 藤原久美子, 鈴木聡, 菅原利夫, 前田初彦, 夏目長門
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 千葉
• [Presentation] Homeobox family HOXC遺伝子は口蓋形成に関与する (2010)
  • Author(s): 平田あずみ, 井村英人, 山田朋弘, 植野高章, 三島克章,南克浩, 夏目長門, 菅原利夫
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 千葉
• [Presentation] 口唇口蓋裂に関する実験的研究-第124報-器官培養による口蓋突起癒合時の観察 (2010)
  • Author(s): 井村英人, 平田あずみ, 山田朋弘, 南克浩, 新美照幸, 古川博雄, 三島克章, 藤原久美子, 鈴木聡, 菅原利夫, 前田初彦, 夏目長門
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] Homeobax family HOXC遺伝子は口蓋形成に関与する (2010)
  • Author(s): 平田あずみ, 井村英人, 山田朋弘, 植野高章, 三島克章, 南克浩, 夏目長門, 菅原利夫
  • Organizer: 第55回日本口腔外科学会総会
  • Place of Presentation: 幕張メッセ(千葉県)
• [Presentation] 口蓋の骨形成過程におけるヘパラン硫酸とヘパラナーゼの役割 (2009)
  • Author(s): 平田あずみ, 山田朋弘, 植野高章, 井村英人, 香川智正, 山近英樹, 松村達志, 森谷徳文, 三島克章, 菅原利夫
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2009-10-10
• [Presentation] 口蓋の骨形成過程におけるヘパラン硫酸とヘパラナーゼの役割 (2009)
  • Author(s): 平田あずみ
  • Organizer: 第54回日本口腔外科学会総会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2009-10-10
• [Presentation] Clinical and histological study in alveolar bone following post-extraction socket augmentation using Master Graft^【〇!R】 granules. (2009)
  • Author(s): Wakimoto M, Ueno T, Hirata A, Shirasu N, Matsumura T, Yamada T, Sugahara T, Aghaloo T, Moy PK.
  • Organizer: European Association for Osseointegration 18th Annual Scientific Meeting
  • Place of Presentation: モナコ
  • Year and Date: 2009-09-29
• [Presentation] Clinical and histological study in alveolar bone following post-extraction socket augmentation using Master Graft^【○!R】granules (2009)
  • Author(s): Wakimoto M
  • Organizer: European Association for Osseointegration 18th Annual Scientific Meeting
  • Place of Presentation: Grimaldi Forwm(モナコ公国)
  • Year and Date: 2009-09-29
• [Presentation] Localization of heparanase during palatal bone formation in palatogenesis in mice. (2009)
  • Author(s): Hirata A, Tsuji T, Ueno T, Yamada T, Imura H, Kagawa T, Matsumura T, Moritani N, Mishima K, Sugahara T, Nakamura N.
  • Organizer: 36th European Symposium on Calcified Tissues
  • Place of Presentation: ウイーン
• [Presentation] Localization of heparanase during palatal bone formation in palatogenesis in mice (2009)
  • Author(s): 平田あずみ
  • Organizer: 36_<th> European Symposium on Calcified Tissues
  • Place of Presentation: Reed Messe Congress Center(オーストリア)
• [Presentation] Heparanase contribute to palate fusion by degrading perlecan. (2008)
  • Author(s): 平田あずみ, 井村英人, 山田朋弘, 植野高章, 山近英樹, 森谷徳文, 松村達志, 香川智正, 三島克章, 菅原利夫
  • Organizer: XIX Congress of the European Association for Cranio-Maxillo-Facial Surgery
  • Place of Presentation: ボローニャ
• [Presentation] Heparanase contribute to palate fusion by degrading perlecan. (2008)
  • Author(s): A. Hirata, H. Imura, T. Yamada, T. Ueno, E. Yamachika, N. Moritani, T. Matsumura, T. Kagawa, K. Mishima, T. Sugahara
  • Organizer: XIX Congress of the European Association for Cranio-MaXillo- Facial Surgery
  • Place of Presentation: Bologna