The cryopreservation of umbilical cord blood derived mesenchymal stem cells and the possibility of clinical application of those cells to cleft lip and palate patient.
| Project/Area Number |
20592345
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kitasato University |
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Principal Investigator |
YAMAZAKI Yasuharu Kitasato University, 医学部, 講師 (00210401)
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| Co-Investigator(Kenkyū-buntansha) |
SEZAKI Koichiro 北里大学, 医学部, 講師 (20216542)
BABA Kyoko 北里大学, 医学部, 助教 (90327411)
AOYAGI Kazuya 北里大学, 医学部, 助教 (10337959)
IKEDA Yasuhiro 北里大学, 医学部, 助教 (20327430)
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| Co-Investigator(Renkei-kenkyūsha) |
OIDA Shinichiro 鶴見大学, 歯学部, 教授 (10114745)
MOCHIDUKI Junko 北里大学, 医学部, 講師 (90306613)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 再生医工学 / 未分化間葉系幹細胞 / 膀帯血 / 贋帯 / 臍帯血 / 臍帯 |
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| Research Abstract |
This research was done with the umbilical cord derived mesenchymal stem cells, because the cord blood derived mesenchymal stem cell couldn't be confirmed. Three months of cryopreservation later, the proliferation potency and multipotency of the umbilical cord derived mesenchymal stem cell were apparently inferior to those of bone marrow derived mesenchymal stem cell. The bone marrow derived mesenchymal stem cell differentiated easily in the osteoblast-like cell under the bone induction medium, but the biological bone viability of the umbilical cord derived mesenchymal stem cell was low, even if bone induction was done with added BMP-2 after the umbilical cord derived mesenchymal cell proliferated with b-FGF. Therefore, as cell source for the regenerated bone, We thought that clinical application of the cryopreserved mesenchymal stem cell from an umbilical cord or blood origin was difficult.
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Report
(4 results)
Research Products
(34 results)
