Analysis of oral mucosal lesion of pemphigus using pathogenic pemphigus monoclonal antibodies

• Project/Area Number: 20592346
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Keio University
• Principal Investigator: TSUNODA Kazuyuki Keio University, 医学部, 助教 (60265915)
• Co-Investigator(Kenkyū-buntansha): NAKAGAWA Taneaki 慶應義塾大学, 医学部, 教授 (00227745)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 天疱瘡 / 自己抗体 / 病原性 / 口腔病変 / デスモグレイン

Research Abstract

Pemphigus vulgaris is an autoimmune blistering disease of oral mucosa. We analyzed pathogenic activity of pemphigus antibodies monoclonal level. In patient serum, the pemphigus autoantibody that has heterogenous pathogenicity was confirmed. We also analyzed the pemphigus IgM autoantibodies as monoclonal level. We confirmed that pemphigus IgM antibody did not have apparent pathogenic activity.
These finding will dissect the pathophysiolosical mechanism of pemphigus.

Research Products

• [Journal Article] 【白い皮膚病】臨床例 口腔粘膜に生じた白色海綿状母斑 (2010)
  • Author(s): 角田和之,加藤伸,大内健嗣,石河晃
  • Journal Title: 皮膚病診療 32 Pages: 955-958
  • Peer Reviewed
• [Journal Article] Subcellular localization of desmosomal components is different between desmoglein3 knockout mice and pemphigus vulgaris model mice (2009)
  • Author(s): Saito H, Shimizu A, Tsunoda K, Amagai M, Ishiko A
  • Journal Title: J Dermatol Sci 55 Pages: 108-115
  • NAID: 10025560737
  • Peer Reviewed
• [Journal Article] Subcellular localization of desmosomal components is different between desmoglein3 knockout mice and pemphigus vulgaris model mice (2009)
  • Author(s): Saito H
  • Journal Title: Journal of Dermatological Science 55 Pages: 108-115
  • Peer Reviewed
• [Journal Article] Autoreactive B-cell elimination by pathogenic IgG specific for the same antigen : implications for peripheral tolerance. (2008)
  • Author(s): Ota T, Aoki-Ota M, Tsunoda K, Nishikawa T, Koyasu S, Amagai M
  • Journal Title: Int Immunol 20 Pages: 1351-1360
  • NAID: 10024357442
  • Peer Reviewed
• [Journal Article] Efficacy of mouth rinse in preventing oral mucositis in patients receiving high-dose cytarabine for allogeneic hematopoietic stem cell transplantation (2008)
  • Author(s): Mori T, Hasegawa K, Okabe A, Tsujimura N, Kawata Y, Yashima T, Kobayashi N, Kondo S, Aisa Y, Kato J, Tsunoda K, Nagai T, Nakagawa T, Shigematsu N, Kubo A, Ikeda Y, Okamoto S
  • Journal Title: Int J Hematol 88 Pages: 583-587
  • NAID: 10023971464
  • Peer Reviewed
• [Journal Article] Autoreactive B-cell elimination by pathogenic IgG specific for the same antigen : implications for peripheral tolerance. (2008)
  • Author(s): Ota T
  • Journal Title: Int Immunol 10 Pages: 1351-1360
  • Peer Reviewed
• [Journal Article] Pathogenic relevance of IgG and IgM anitbodies against desmoglein 3 in blister formation in pemphigus vulgaris
  • Author(s): Tsunoda K, Ota T, Saito M, Hata T, Shimizu A, Ishiko A, Yamada T, Nakagawa T, Kowalczyk AP, Amagai M
  • Journal Title: Am J Pathol (in press)
  • Peer Reviewed
• [Presentation] Keratin 13 gene mutation cases oral white sponge nevus. (2010)
  • Author(s): Tsunoda K, Kato S, Ouchi T, Ishiko A
  • Organizer: 37th Annual Meeting of the SCUR
  • Place of Presentation: Helsinki, Finland(poster presentation)
• [Presentation] 口腔内増殖性天疱瘡が疑われた1例 (2010)
  • Author(s): 小池晶彦,宮下英高,高森康次,木村彩,角田和之,池内忍
  • Organizer: 第20 回日本口腔粘膜学会総会
  • Place of Presentation: 大阪(口頭)
• [Presentation] ケラチン13遺伝子変異により生じた白色海綿状母斑の1例 (2010)
  • Author(s): 宇賀允悠,角田和之,加藤伸,小高利絵,角田博之,大内健嗣,石河晃,中川種昭
  • Organizer: 第20回日本口腔粘膜学会総会
  • Place of Presentation: 大阪(口頭)
• [Presentation] Keratin 13 gene mutation cases oral white sponge nevus (2010)
  • Author(s): Tsunoda K
  • Organizer: 37th Annual Meeting of the Society for Cutaneous Ultrastructural Research
  • Place of Presentation: Helsinki
• [Presentation] ケラチン13遺伝子変異により生じた白色海綿状母斑の1例 (2010)
  • Author(s): 宇賀允悠
  • Organizer: 第20回日本口腔粘膜学会総会
  • Place of Presentation: 大阪
• [Presentation] 口腔内増殖性天疱瘡が疑われた1例 (2010)
  • Author(s): 小池晶彦
  • Organizer: 第20回日本口腔粘膜学会総会
  • Place of Presentation: 大阪
• [Presentation] 口腔粘膜疾患のトランスレーショナルリサーチ天疱瘡の基礎研究とその臨床応用 (2009)
  • Author(s): 角田和之
  • Organizer: 第19回日本口腔粘膜学会総会・学術集会
  • Place of Presentation: 神奈川県三浦郡(シンポジウム)
• [Presentation] 粘膜類天疱瘡の1例 (2009)
  • Author(s): 小池晶彦,角田和之,柴秀行,新里知佳,加藤伸,永井哲,天谷雅行,中川種昭
  • Organizer: 第19回日本口腔粘膜学会総会・学術集会
  • Place of Presentation: 神奈川県三浦郡(口頭)
• [Presentation] Binding kinetic analysis of anti-desmoglein 3 monoclonal antibodies by Biacore. (2009)
  • Author(s): Tsunoda K, Nakagawa T, Amagai M
  • Organizer: 6th General Session & Exhibition of the International Association for Dental Research
  • Place of Presentation: Miami, USA(oral presentation)
• [Presentation] 天疱瘡の病原性エピトープを認識する抗デスモグレイン3IgM抗体の病原性解析 (2009)
  • Author(s): 角田和之、中川種昭
  • Organizer: 第63回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 浜松(口頭)
• [Presentation] Binding kinetic analysis of anti-desmoglein 3 monoclonal antibodies by Biacore (2009)
  • Author(s): 角田和之
  • Organizer: International association for dental research
  • Place of Presentation: Miami
• [Presentation] 天疱瘡の病原性エピトープを認識する抗デスモグレイン3 IgM抗体の病原性解析 (2009)
  • Author(s): 角田和之
  • Organizer: 第63回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 浜松
• [Presentation] 天疱瘡の基礎研究とその臨床応用(招待講演・シンポジスト) (2009)
  • Author(s): 角田和之
  • Organizer: 第19回日本口腔粘膜学会総会・学術集会
  • Place of Presentation: 葉山
• [Presentation] 粘膜類天疱瘡の1例 (2009)
  • Author(s): 小池晶彦
  • Organizer: 第19回日本口腔粘膜学会総会・学術集会
  • Place of Presentation: 葉山
• [Presentation] White sponge nevus の一例 (2009)
  • Author(s): 角田和之
  • Organizer: 第36回皮膚かたち研究学会
  • Place of Presentation: 宮崎
• [Presentation] 天疱瘡モノクローナル抗体の結合カイネティクス解析による新たな病原性因子の探索 (2008)
  • Author(s): 角田和之,中川種昭,天谷雅行
  • Organizer: 第53回日本口腔外科学会
  • Place of Presentation: 徳島(口頭)
• [Presentation] デスモグレイン3の天疱瘡病原性エピトープを認識するIgM抗体の作製とその病原性解析 (2008)
  • Author(s): 角田和之,畑毅,清水篤,石河晃,山田健人,中川種昭,天谷雅行
  • Organizer: 日本口腔粘膜学会雑誌
  • Place of Presentation: 東京(口頭)
• [Presentation] IgM to IgG isotype switching of anti-desmoglein 3 antibodies is an essential step for pathogenesis of pemphigus vulgaris. (2008)
  • Author(s): Tsunoda K, Ota T, Hata T, Shimizu A, Yamada T, Nakagawa T, Amagai M
  • Organizer: International Investigative Dermatology, annual meeting
  • Place of Presentation: Kyoto(poster presentation)
• [Presentation] 口腔粘膜にびらん、潰瘍を生じる疾患 (2008)
  • Author(s): 角田和之
  • Organizer: 日本皮膚科学会総会
  • Place of Presentation: 京都(教育講演)
• [Presentation] IgM to IgG isotype switching of anti-desmoglein 3 antibodies is an essential step for pathogenesis of pemphigus vulgaris (2008)
  • Author(s): Kazuyuki Tsunoda
  • Organizer: International investigative dermatology
  • Place of Presentation: Kyoto
• [Presentation] デスモグレイン3の天疱瘡病原性エピトープを認識するIgM抗体の作成とその病原性解析 (2008)
  • Author(s): 角田和之
  • Organizer: 第18回日本口腔粘膜学会
  • Place of Presentation: 東京
• [Presentation] 天疱瘡モノクローナル抗体を用いた結合カイネティクス解析による新たな病原性因子の探索 (2008)
  • Author(s): 角田和之
  • Organizer: 第53回日本口腔外科学会総会
  • Place of Presentation: 徳島
• [Book] 医師・歯科医師のための口腔診療必携(口腔粘膜に水疱・びらん・剥離ができている患者が来院したら?) (2010)
  • Author(s): 角田和之,天谷雅行
  • Total Pages: 10
  • Publisher: 金原出版
• [Book] 小児歯科臨床(小児の口腔粘膜疾患 15) (2010)
  • Author(s): 加藤伸,新里知佳,角田和之,永井哲夫
  • Publisher: 全国小児歯科開業医会
• [Book] 医師・歯科医師のための口腔診療必携 (2010)
  • Author(s): 角田和之
  • Total Pages: 2
  • Publisher: 金原出版
• [Book] 口腔外科ハンドマニュアル'08 天疱瘡・類天疱瘡 (2008)
  • Author(s): 角田和之
  • Publisher: クインテッセンス出版
• [Book] 口腔外科ハンドマニュアル'08天疱瘡・類天疱瘡 (2008)
  • Author(s): 角田和之
  • Publisher: クインテッセンス出版
• [Remarks] ホームページ等