Type of bone invasion in human or al squamous cell carcinoma and its mechanisms ; application for molecular diagnosis

• Project/Area Number: 20592356
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: University of Toyama
• Principal Investigator: NOGUCHI Makoto University of Toyama, 大学院・医学薬学研究部(医学), 教授 (50208328)
• Co-Investigator(Kenkyū-buntansha): ARAI Naoya 富山大学, 大学院・医学薬学研究部(医学), 准教授 (80323723)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔扁平上皮癌 / 顎骨浸潤 / 破骨細胞 / 破骨細胞分化誘導因子 / ビスホスホネート製剤 / アポトーシス / 扁平上皮癌 / ピスホスホネート製 / ピスホスホネート製剤 / アポートシス

Research Abstract

We immunohistochemically observed expression of various kinds of cytokines related to osteoclastic differentiation in human squamous cell carcioma of the lower gum. Over-expression of RANKL or OPG could be barely seen in cancer cells, although PTHrP was slightly expressed. The relationship between type of bone invasion and expression of them has failed to clarify.
Using oral squamous cancer cell lines, over-expression of MMP9 or periostin was strongly observed in high invasive cell line (HSC4) compared with low invasive cell line (HSC3). Apoptosis was induced in HSC4 cells with low dose bisphosphonate. Moreover, induction of apoptosis in cell line was enhanced by addition to Ca.

Research Products

• [Journal Article] Heparin induces apoptosis through suppression of AKt in oral squamous cell carcinoma cells. (2009)
  • Author(s): Ueda K., Inoue S., Zhang Y., Kutsuna T., Inoue S., Noto K., Arai N., Noguchi M.
  • Journal Title: Anticancer research 29 Pages: 1079-1088
  • Peer Reviewed
• [Journal Article] Heparin induces apoptosis through suppression of Akt in oral squamous Cell carcinoma cells (2009)
  • Author(s): UEDA, K., et al.
  • Journal Title: Anticancer Research 29 Pages: 1079-1088
  • Peer Reviewed
• [Journal Article] Activation of MAP kinases, Akt and PDGF receptors in injured peripheral nerves. (2009)
  • Author(s): YAMAZAKI, T., et al.
  • Journal Title: J Peripheral Nernous Sys 14 Pages: 165-176
  • Peer Reviewed
• [Journal Article] Heparin indues apoptosis through suppression of Akt in oral squamous cell carcinoma cells (2009)
  • Author(s): K. UEDA, M NOGUCHI, et al
  • Journal Title: Anticancer Research 29(掲載確定)
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌細胞株における低濃度ビスフォスフォの抗腫瘍効果 (2011)
  • Author(s): 井上さやか、新井直也、野口誠
  • Organizer: 第65回日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-22
• [Presentation] A strategy for organ preservation in treating of locally advanced oral cancer. (2010)
  • Author(s): Noguchi M., Tsuno H., Imaue S., Ohura T., Inoue S., Arai N.
  • Organizer: 9^<th> Asian congress onoral & maxillofacial surgery Kuala Lumpur
  • Place of Presentation: Malaysia
  • Year and Date: 2010-10-26
• [Presentation] A strategy for organ preservation in treating locally advanced oral cancer (2010)
  • Author(s): NOGUCHI M., et al.
  • Organizer: Asian Congress on Oral and Maxillofacial Surgery
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2010-08-27
• [Presentation] 上顎前歯部に発生した角化襄胞性歯原性腫瘍の1例 (2008)
  • Author(s): 井上さやか、朽名智彦、金佳美、能登久美子、井上秀一、新井直也、野口誠
  • Organizer: 第33回日本口腔外科学会中部地方会
  • Place of Presentation: 於岐阜
  • Year and Date: 2008-05-24
• [Presentation] 下顎歯肉扁平上皮癌の臨床病理学的研究. (2008)
  • Author(s): 能登久美子、朽名智彦、金佳美、上田耕平、井上さやか、野口誠
  • Organizer: 第26回日本口腔腫瘍学会総会
  • Place of Presentation: 於大分
  • Year and Date: 2008-01-25