| Project/Area Number |
20592369
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
YOSHIZAWA Michiko Niigata University, 医歯学系, 助教 (60303137)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Yukiko 新潟大学, 医歯学総合病院, 助教 (80345511)
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| Co-Investigator(Renkei-kenkyūsha) |
TERASHI Hiroto 神戸大学, 医学部附属病院, 准教授 (80217421)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 口腔顎顔面再建外科学 / 再生医療 / 歯学 / 口腔粘膜 |
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| Research Abstract |
The objective of the present study was to investigate the role of grafted human oral keratinocytes in a transplanted ex vivo-produced oral mucosa equivalent (EVPOME) in the regeneration and/or healing process of the oral mucosa at the recipient site of mice. The grafted EVPOME maintained a stratified epithelial layer and basement membrane for up to 5 days post-grafting. By day 7 post-grafting, a portion of the EVPOME epithelial layer peeled away from the AlloDerm^[○!R]R and a thin, CK17-immunonegative epithelial layer extended from the adjacent thick epithelia layer of the mouse and contacted the CK17 immunopositive EVPOME epithelium. These findings suggest that grafting of EVPOME with viable oral keratinocytes onto an intraoral mucosal wound plays an active role in promotion of re-epithelialization of the oral wound during the subsequent healing process.
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