| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
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| Research Abstract |
The role of substance P (SP) in general anesthetic action was examined. In in vivo study, general anesthetic action was evaluated in mice using an anesthetic end point : loss of movement in response to noxious stimulation (as a measure of immobility). In in vitro SP release study, primary afferent neurons prepared from Wistar rat dorsal root ganglion (DRG) cells were cultured. Morphine is known to inhibit SP release from primary afferent neurons. The ED_95 of morphine of analgesia significantly enhanced immobility induced by pentobarbital, an intravenous anesthetic agent. Pentobarbital suppressed the SP release evoked by capsaicin in a concentration-dependent fashion. These findings suggest that the pentobarbital-induced immobility may involve the suppression of SP release from primary afferent neurons.
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