Analysis of mutans streptococci signal transduction system in biofilm formation

• Project/Area Number: 20592399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Osaka University
• Principal Investigator: NAKANO Michiyo Osaka University, 大学院・歯学研究科, 准教授 (30359848)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ミュータンスレンサ球菌 / Streptococcus mutans / う蝕 / シグナル伝達システム / 遺伝子 / バイオフィルム / クオラムセンシング / 調節遺伝子 / グルコシルトランスフェラーゼ / 分子生物学 / 遺伝子解析

Research Abstract

Quorum sensing has been reported to mediate biofilm formation by Streptococcus mutans. Recombinase A (RecA) encoded by the recA gene, is essential for transformation of both plasmids and chromosomal DNA. We found that the expression intensity of glucosyltransferases was reduced and biofilm structure altered in an RecA-deficient mutant strain. Thus, RecA was shown to have strong relationships with biofilm formation and quorum sensing.

Research Products

• [Journal Article] Inhibitory effect of Oenothera biennis (Evening primrose) seed extract on Streptococcus mutans and S.mutans-induced dental caries in rats (2011)
  • Author(s): Matsumoto-Nakano M, Nagayama K, Kitagori H, Inagaki S, Takashima Y, Tamesada M, Kawabata S, Ooshima T.
  • Journal Title: Caries Res 45 Pages: 56-63
  • Peer Reviewed
• [Journal Article] Correlation of biological properties with glucan-binding protein B expression profile in Streptococcus mutans clinical isolates. (2011)
  • Author(s): Fujita K, Takashima Y, Inagaki S, Nagayama K, Nomura R, Ardin AC, Gronroos L, Alaluusua S, Ooshima T, Matsumoto-Nakano M
  • Journal Title: Arch Oral Biol 56 Pages: 258-263
  • Peer Reviewed
• [Journal Article] Inhibitory effects of Oenothera biennis(Evening Primrose) seed extract on Streptococcus mutans and S.mutans-induced dental caries in rats. (2011)
  • Author(s): Matsumoto-Nakano, et al.
  • Journal Title: Caries Research Volume: 45 Pages: 56-63
  • Peer Reviewed
• [Journal Article] Correlation of biological properties with glucan-binding protein B expression profile in Streptococus mutans clinical isolates. (2011)
  • Author(s): Fujita K, Takashima Y, Inagaki S, Nagayama K, Nomura R, Ardin AC, Gronroos L, Alaluusua S, Ooshima T, Matsumoto-Nakano M
  • Journal Title: Archives of Oral Biology Volume: 56 Pages: 258-263
  • Peer Reviewed
• [Journal Article] Detection of oral streptococci with collagen-binding properties in saliva specimens from mothers and their children. (2010)
  • Author(s): Nomura R, Naka S, Nakano K, Taniguchi N, Matsumoto M, Ooshima T.
  • Journal Title: Int J Pediatric Dent 20 Pages: 254-260
  • Peer Reviewed
• [Journal Article] Roles of oral bacteria in cardiovascular disease-from molecular mechanisms to clinical cases : Cell-surfaces of novel serotype k Streptococcus mutans strains and their correlation to virulence. (2010)
  • Author(s): Nakano K, Nomura R, Matsumoto M, et al.
  • Journal Title: Journal of Pharmacogical Science Volume: 113 Pages: 120-125
  • Peer Reviewed
• [Journal Article] Contribution of cell surface protein antigen c of Streptococcus mutans to platelet aggregation. (2009)
  • Author(s): Matsumoto-Nakano M, Tsuji M, Inagaki S, Fujita K, Nagayama K, Nomura R, Ooshima T.
  • Journal Title: Oral Microbiol Immunol 24 24 Pages: 427-430
  • Peer Reviewed
• [Journal Article] Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans. (2009)
  • Author(s): Inagaki S, Matsumoto-Nakano M, Fujita K, Nagayama K, Funao J, Ooshima T.
  • Journal Title: Oral Microbiol Immunol 24 Pages: 104-108
  • Peer Reviewed
• [Journal Article] Bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from Japanese subjects. (2009)
  • Author(s): Miyamoto E, Nakano K, Fujita K, Nomura R, Okawa R, Matsumoto M, Ooshima T.
  • Journal Title: Arch Oral Biol 54 Pages: 374-379
  • Peer Reviewed
• [Journal Article] Contribution of cell surface protein antigen c of Streptococcus mutans to platelet aggregation (2009)
  • Author(s): Matsumoto-Nakano M, Tsuji M, Inagaki S, Fujita K, Nagayama K, Nomura R, Ooshima T.
  • Journal Title: Oral Microbiology and Immunology 24 Pages: 427-430
  • Peer Reviewed
• [Journal Article] Effects of recombinase A deficiency on biofilm formation by Streptococcus mutans (2009)
  • Author(s): Inagaki S, Matsumoto-Nakano M, Fujita K, Nagayama K, Funao J, Ooshima T.
  • Journal Title: Oral Microbiology and Immunology 24 Pages: 104-108
  • Peer Reviewed
• [Journal Article] Molecular interactions of alanine-rich and proline-rich regions of cell surface protein antigen c in Streptococcus mutans. (2008)
  • Author(s): Matsumoto-Nakano M, Tsuji M, Amano A, Ooshima T.
  • Journal Title: Oral Microbiol Immunol 23 Pages: 265-270
  • Peer Reviewed
• [Journal Article] Streptococcus mutansにお けるbacteriocin immunity proteinによる抗生物質感受性メカニズムの解析 (2008)
  • Author(s): 松本道代
  • Journal Title: 小児歯科学雑誌 46 Pages: 501-504
• [Journal Article] Molecular interaction of alanine-rich and proline-rich regions of cell surface protein antigen c in Streptococcus mutans. (2008)
  • Author(s): Matsumoto-Nakano M, Tsuji M, Amano A, Ooshima T
  • Journal Title: Oral Microbiology and Immunology 23 Pages: 265-270
  • Peer Reviewed
• [Presentation] GTF activity concerning the RecA protein of Streptococcus mutans for the clinical isolates (2011)
  • Author(s): 稲垣暁子, 松本道代, 藤田一世, 永山佳代子, 高島由紀子, 大嶋隆
  • Organizer: 89th IADR meeting
  • Place of Presentation: San Diego, USA.
  • Year and Date: 2011-03-18
• [Presentation] Functional domain of glucan-binding protein C of Streptococcus mutans (2011)
  • Author(s): Matsumoto-Nakano M, Takashima Y, Fujita K, Inagaki S, Nagayama K, Nomura R, Ooshima T
  • Organizer: 89th IADR meeting
  • Place of Presentation: San Diego, USA.
  • Year and Date: 2011-03-18
• [Presentation] Functional analysis of ABC transporter proteins of Streptococcus mutans (2011)
  • Author(s): 永山佳代子, 松本道代, 稲垣暁子, 藤田一世, 高島由紀子, 大嶋隆
  • Organizer: 89th IADR meeting
  • Place of Presentation: San Diego, USA.
  • Year and Date: 2011-03-18
• [Presentation] Functional domain of glucan-binding Protein C of Streptococcus mutans. (2011)
  • Author(s): Natsumoto-Nakano M, et al.
  • Organizer: 89th IADR meeting
  • Place of Presentation: SanDiego, USA
  • Year and Date: 2011-03-18
• [Presentation] Streptococus mutans RecAタンパクのグルカン合成酵素の活性に及ぼす影響 (2010)
  • Author(s): 稲垣暁子, 松本道代, 藤田一世, 永山佳代子, 高島由紀子, 大嶋隆
  • Organizer: 平成22年度日本小児歯科学会秋季大会
  • Place of Presentation: 郡山
  • Year and Date: 2010-12-02
• [Presentation] Characterization of glucan-binding protein B of Streptococcus mutans clinical isolates. (2010)
  • Author(s): 藤田一世, 松本道代, 高島由紀子, 稲垣暁子, 永山佳代子, 大嶋隆
  • Organizer: 88th IADR meeting
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 2010-07-17
• [Presentation] Contribution of RecA protein to GTF activity of Streptococcus mutans (2010)
  • Author(s): 稲垣暁子, 松本道代, 藤田一世, 永山佳代子, 高島由紀子, 大嶋隆
  • Organizer: 88th IADR meeting
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 2010-07-17
• [Presentation] Characterization or glucan-binding protein B of Streptococcus mutansclinical isolates. (2010)
  • Author(s): Fujita K, Matsumoto-Nakano M, et al.
  • Organizer: 88th IADR meeting
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-07-14
• [Presentation] Anticariogenic effects of Oenothera biennis seed extract on Streptococcus mutans activity (2010)
  • Author(s): Matsumoto-Nakano M, Nagayama K, Fujita K, Inagaki S, Takashima Y, Kawabata S, Ooshima T
  • Organizer: 57th ORCA congress
  • Place of Presentation: Montpellier, France.
  • Year and Date: 2010-07-07
• [Presentation] Anticariogenic effects of Oenothera biennis seed extracts on Sreptocaccus mutans (2010)
  • Author(s): Matsumoto-Nakano M, et al.
  • Organizer: 57th ORCA congress
  • Place of Presentation: Montpellier, France
  • Year and Date: 2010-07-07
• [Presentation] Streptococcus mutansにおけるATP結合タンパクの役割 (2010)
  • Author(s): 永山佳代子、松本道代、稲垣暁子、大嶋隆
  • Organizer: 第48回日本小児歯科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-05-19
• [Presentation] Streptococcus mutansのGTF活性に対するRecAタンパクの発現の影響 (2009)
  • Author(s): 稲垣暁子, 松本道代, 藤田一世, 永山佳代子, 高島由紀子, 大嶋隆
  • Organizer: 第28回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-18
• [Presentation] Functional analysis of ATP transporter proteins associated with antibiotic resistance in Streptococcus mutans (2009)
  • Author(s): Matsumoto-Nakano M, Nagayama K, Inagaki S, Fujita K, Takashima Y, Ooshima T.
  • Organizer: 22nd Meeting of International Association of Pediatric Dentistry
  • Place of Presentation: Munich, Germany
  • Year and Date: 2009-06-17
• [Presentation] Contribution of recA gene for gtf expression of Streptococcus mutans (2009)
  • Author(s): 稲垣暁子, 松本道代, 藤田一世, 永山佳代子, 高島由紀子, 大嶋隆
  • Organizer: 22nd IAPD meeting
  • Place of Presentation: Munich, Germany.
  • Year and Date: 2009-06-16
• [Presentation] Functional analysis of ATP transporter proteins associated with antibiotic resistance in Streptococcus mutans. (2009)
  • Author(s): 松本道代, 永山佳代子, 稲垣暁子, 藤田一世, 高島由紀子, 大嶋隆
  • Organizer: 22nd IAPD meeting
  • Place of Presentation: Munich, Germany.
  • Year and Date: 2009-06-16
• [Presentation] Correlation of biological properties and expression profile of glucan-binding protein B in Streptococcus mutans clinical isolates (2009)
  • Author(s): 藤田一世, 松本道代, 高島由紀子, 稲垣暁子, 大嶋隆
  • Organizer: 22nd IAPD meeting
  • Place of Presentation: Munich, Germany.
  • Year and Date: 2009-06-16
• [Presentation] Streptococcus mutans RecAタンパクのgtf遺伝子発現制御における分子生物学的検討 (2009)
  • Author(s): 稲垣暁子, 松本道代, 大嶋隆
  • Organizer: 第47回日本小児歯科学会大会
  • Place of Presentation: 吹田
  • Year and Date: 2009-05-14
• [Presentation] Streptococcus mutansの表層タンパク欠失におけるバイオフィルム構造の比較 (2009)
  • Author(s): 藤田一世, 稲垣暁子, 松本道代, 大嶋隆
  • Organizer: 第47回日本小児歯科学会大会
  • Place of Presentation: 吹田
  • Year and Date: 2009-05-14
• [Presentation] Streptococcus mutansにおけるstress response regulatorの分子生物学的解明 (2009)
  • Author(s): 永山佳代子, 松本道代, 稲垣暁子, 藤田一世, 大嶋隆
  • Organizer: 第47回日本小児歯科学会大会
  • Place of Presentation: 吹田
  • Year and Date: 2009-05-14
• [Presentation] バイオフィルム形成におけるStreptococcus mutans recA遺伝子の機能解析 (2008)
  • Author(s): 稲垣暁子, 松本道代, 大嶋隆
  • Organizer: 第27回日本小児歯科学会近畿地方会大会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-19
• [Presentation] Deficiency of glucan-binding proteins alters gene expression ofStreptococcus mutans. (2008)
  • Author(s): Matsumoto-Nakano M, Fujita K, Ooshima T.
  • Organizer: 86th IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-05
• [Presentation] Streptococcus mutans recA遺伝子によるグルカン合成酵素の発現調節機能 (2008)
  • Author(s): 稲垣暁子, 松本道代, 藤田一世, 大嶋隆
  • Organizer: 第46回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12
• [Presentation] Streptococcus mutansのバイオフィルム形成における遺伝子修復機能について (2008)
  • Author(s): 藤田一世, 松本道代, 大嶋隆
  • Organizer: 第46回日本小児歯科学会大会
  • Place of Presentation: さいたま
  • Year and Date: 2008-06-12