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IL-17刺激ヒト歯根膜線維芽細胞におけるサイトカインの動態

Research Project

Project/Area Number 20592400
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionOsaka University

Principal Investigator

上松 節子  大阪大学, 歯学部・附属病院, 講師 (80271378)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsIL-17 / 歯根膜細胞 / MMP-1 / IL-6 / PDL
Research Abstract

1. ヒト歯根膜線維芽細胞の培養
細胞は、10%牛胎児血清(FBS)を添加したダルベッコ変法イーグル培地(DMEM)を用いて、5%CO_2、37℃で培養した。
2. RT-PCR法によるRANKLならびにMMP-1 mRNAの発現の検索
IL-6ならびにsIL-6の添加時間について段階的に変更して検討し添加時間の範囲を決定した。

Report

(3 results)
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (1 results)

All 2009

All Journal Article (1 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] The outcome of a two-phase treatment in a patient with Angle Class II division 1 malocclusion and an excessive overjet2009

    • Author(s)
      Takigawa Y, Sanma Y, Uematsu S, Takada K
    • Journal Title

      Orthodontic Waves 68

      Pages: 88-94

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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