| Project/Area Number |
20592408
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
FUKUNAGA Tomohiro Kagoshima University, 病院, 講師 (70362994)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Nobuo 東北大学, 大学院・歯学研究科, 助教 (50431515)
前田 綾 鹿児島大学, 大学院・医歯学総合研究科, 助教 (10457666)
山本 芳丈 鹿児島大学, 医学部・歯学部附属病院, 助教 (50380465)
宮脇 正一 鹿児島大学, 大学院・医歯学総合研究科, 教授 (80295807)
飯野 祥一朗 鹿児島大学, 医学部・歯学部附属病院, 助教 (10295256)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | マウス下顎頭軟骨 / Ten-m / Odz3 / 細胞分化 / 遺伝子発現 / Ten-m/Odz3 / odz3 |
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| Research Abstract |
The mandibular condyle has been speculated to develop via the differentiation of the fibroblast-like cells covering the condyle into chondrocytes ; however, the developmental mechanisms behind this process have not been revealed. We describe the temporal and spatial expression of Ten-m/Odz3 mRNA as determined by in situ hybridization in mouse mandibular condylar cartilage of 1 day, 1 week, and 3 week of age during the early stages of development. Ten-m/Odz3 was expressed in the fibrous layer and the proliferating and mature chondrocyte layers, but was not detected in the hypertrophic chondrocyte layer. Furthermore, we evaluated the in vitro expression of Ten-m/Odz3 using ATDC5 cells, a mouse chondrogenic cell line. Ten-m/Odz3 was expressed during the early stage of the differentiation of mesenchymal cells into chondrocytes. These findings suggest that Ten-m/Odz3 is involved in the differentiation of chondrocytes and that it acts as a regulatory factor in the early stages of the development of mandibular condylar cartilage.
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