Culture of human dental stem cells from human mandibular third molars and its application of the recombinant tooth germ
Project/Area Number |
20592411
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthodontic/Pediatric dentistry
|
Research Institution | Iwate Medical University |
Principal Investigator |
MAYAMA Hisayo Iwate Medical University, 歯学部, 助教 (90382639)
|
Co-Investigator(Renkei-kenkyūsha) |
HARADA Hidemitu 岩手医科大学, 歯学部, 教授 (70271210)
FUJIWARA Naoki 岩手医科大学, 歯学部, 講師 (20190100)
MIURA Hiroyuki 岩手医科大学, 歯学部, 教授 (00048563)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 歯学 / 再生医学 / 歯の再生 / 細胞・組織 / biotooth / ヒト下顎第三大臼歯 / 歯胚 / 間葉系細胞 |
Research Abstract |
We attempted to isolate and culture of human dental epithelial stem cells and mesenchymal stem cells, and we examined the possibility of human tooth regeneration using these dental epithelial and mesenchymal stem cells. In order to investigate the possibility of tooth regeneration using these cells, we transplanted the recombinant dental lamina epithelial cells and dental papilla into the abdominal cavities of SCID mice. The epithelial cells had developed into dental epithelium resembling the bud stage. The present results suggest that human dental lamina cells are a useful source of dental epithelial stem cells for human tooth regeneration.
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Report
(4 results)
Research Products
(6 results)