Identification for TGF-beta signaling during palatal development
| Project/Area Number |
20592415
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
NAKAJIMA Akira Nihon University, 歯学部, 助教 (50297842)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | TGF-beta / 口蓋裂 / 二次口蓋 / 分子生物学 / 成長・発育 |
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| Research Abstract |
The molecular mechanisms regulating palatogenesis remain incompletely characterized but are most likely to be involved in the molecular etiology for cleft palate. The objective was to investigate TGF-β3 signaling when both TGF-β type II and III receptors (TβR-II/III) were knocked down an NIH3T3 cell line and palatal organ culture in vitro. Treatment with siTβR-II/III reduced the expression of the target genes. The treated palates were partially fused at E13+72h when TβR-II/III were knocked down,although all control palate were completely fused. The downstream genes was decreased in the siTβR-II/III treated NIH3T3 cells and palatal shelves. The present study demonstrated that knocking down both TβR-II and III could affect the downstream signaling pathway of TGF-β by reducing the levels of phospho-Smad2. The siTβR-II/III treatment resulted in persistent MEE cell proliferation, which has been shown to be linked to a failure to complete palatal fusion events.
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Report
(4 results)
Research Products
(19 results)
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[Journal Article]2011
Author(s)
Nakajima A, Tsuboi Y, Suzuki I, Honda K, Shinoda M, Kondo M, Matsuura S, Shibuta K, Yasuda M, Shimizu N, Iwata K
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Journal Title
Journal of Dental Research (In Press)
Related Report
Peer Reviewed
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[Journal Article] Protein kinase Cγ expression in the trigeminal subnucleus caudalis and upper cervical spinal cerord is involved in trigeminal neuropathic pain in rats2011
Author(s)
Nakajima A, Tsuboi Y, Suzuki I, Honda K, Shinoda M, Kondo M, Matsuura S, Shibuta K, Yasuda M, Shimizu N, Iwata K
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Journal Title
Journal of Dental Research
Volume: In Press
Related Report
Peer Reviewed
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[Presentation]2009
Author(s)
中嶋昭
Organizer
Current orthodontics
Place of Presentation
徳島大学歯学部,徳島(徳島大学招待講演)
Related Report
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