| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
The present study examined whether amelogenin activates the cell differentiation in mouse osteoblasts and human periodontal ligament (PDL) cells and used the T7 phage-display osteoblast library to identify a target protein with which amelogenin can interact. The marker genes of osteoblast-phenotype were enhanced by amelogenin in the osteoblasts. However, these genes were suppressed in the PDL cells. The expression of RANKL, a critical regulator of osteoclastogenesis was increased in the osteoblasts and PDL cells. The DNA sequence that demonstrated one of the highest affinities for amelogenin was tissue inhibitor of metalloproteinase-2 (TIMP-2). These findings suggest that amelogenin can induce cell differentiation of osteoblasts and may facilitate the mineralization procedure during matrix formation by increasing the MMP activities.
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