Cell-patterning technology for epithelial rest of Malassez network and its application for periodontal regeneration

• Project/Area Number: 20592423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: KOMAKI Motohiro Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 准教授 (30401368)
• Co-Investigator(Renkei-kenkyūsha): MORITA Ikuo 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (60100129) ; ABE Mayumi 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (80271980) ; IZUMI Yuichi 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (60159803)
• Research Collaborator: IWASAKI Kengo 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40401351)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周組織再生 / 細胞パターニング / セメント芽細胞 / 羊膜への細胞転写技術 / CEMP1 / 骨髄由来細胞 / ヒト歯根膜細胞 / 細胞転写技術

Research Abstract

A periodontal disease is an inflammatory disease due to bacterial infection and is a main cause of tooth loss. Therefore, development of the safe and effective regenerative therapy is an extremely important issue. However, conventional treatment such as the GTR method or the enamel protein does not lead to complete periodontal regeneration. We performed basic evaluation for the purpose of establishment of the periodontal regeneration with the new cementum formation to the root surface and confirmed the effectiveness of the bone regeneration with the cell equipped-carrier and clarified the function of the molecules that participated in cementoblast differentiation.

Research Products

• [Journal Article] Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells. (2011)
  • Author(s): Komaki M, Iwasaki K, Arzate H, Sampath Narayanan A, Izumi Y, Morita I
  • Journal Title: J Cell Physiol. in press
• [Journal Article] Cell-printing and transfer technology applications for bone defects in mice. (2011)
  • Author(s): Tsugawa J, Komaki M, Yoshida T, Nakahama KI, Amagasa T, Morita I
  • Journal Title: J Tissue Eng Regen Med. in press
• [Journal Article] Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods. (2011)
  • Author(s): Tanaka K, Iwasaki K, Feghali KE, Komaki M, Ishikawa I, Izumi Y
  • Journal Title: Arch Oral Biol. 56(4)
• [Journal Article] Cementum protein 1 (CEMP1) induces a cementoblastic phenotype and reduces osteoblastic differentiation in periodontal ligament cells. (2011)
  • Author(s): Komaki M, Iwasaki K, et al.
  • Journal Title: J Cell Physiol. Volume: in press
  • Peer Reviewed
• [Journal Article] Cell-printing and transfer technology applications for bone defects in mice. (2011)
  • Author(s): Tsugawa J, Komaki M, et al.
  • Journal Title: J Tissue Eng Regen Med. Volume: in press
  • Peer Reviewed
• [Journal Article] Comparison of characteristics of periodontal ligament cells obtained from outgrowth and enzyme-digested culture methods. (2011)
  • Author(s): Y Tanaka K, Iwasaki K, Feghali KE, Komaki M, et al.
  • Journal Title: Arch Oral Biol. Volume: 56(4) Pages: 380-388
• [Journal Article] Therapeutic angiogenesis by implantation of a capillary structure constituted of human adipose tissue microvascular endothelial cells. (2010)
  • Author(s): Yoshida T, Komaki M, Hattori H, Negishi J, Kishida A, Morita I, Abe M
  • Journal Title: Arterioscler Thromb Vasc Biol. 30(7)
• [Journal Article] Implantation of capillary structure engineered by optical lithography improves hind limb ischemia in mice. (2010)
  • Author(s): Akahori T, Kobayashi A, Komaki M, Hattori H, Nakahama K, Ichinose S, Abe M, Takeda S, Morita I
  • Journal Title: Tissue Eng Part A. 16(3)
• [Journal Article] Therapeutic angiogenesis by implantation of a capillary structure constituted of human adipose tissue microvascular endothelial cells. (2010)
  • Author(s): Yoshida T, Komaki M, et al.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 30(7) Pages: 1300-1307
  • Peer Reviewed
• [Journal Article] Implantation of capillary structure engineered by optical lithography improves hind limb ischemia in mice. (2010)
  • Author(s): Akahori T, et al.
  • Journal Title: Tissue Eng Part A 16(3) Pages: 953-9
  • Peer Reviewed
• [Journal Article] Human gingival fibroblasts release high-mobility group box-1 protein through active and passive pathways. (2009)
  • Author(s): Feghali K, et al.
  • Journal Title: Oral Microbiol Immunol 24(4) Pages: 292-8
  • Peer Reviewed
• [Journal Article] A comparison of the tube forming potentials of early and late endothelial progenitor cells. (2008)
  • Author(s): Mukai N, Akahori T, Komaki M, Li Q, Kanayasu-Toyoda T, Ishii-Watabe A, Kobayashi A, Yamaguchi T, Abe M, Amagasa T, Morita I
  • Journal Title: Exp Cell Res. 314(3)(Epub 2007 Nov 29)
• [Journal Article] IL-6 Induces Osteoblastic Differentiation of Periodontal Ligament Cells. (2008)
  • Author(s): Iwasaki K
  • Journal Title: J Dent Res. 87(10) Pages: 937-942
  • Peer Reviewed
• [Journal Article] A comparendothelson of the tube forming potentials of early and lateal progeni tor eel Is. (2008)
  • Author(s): Mukai N
  • Journal Title: Exp Cell Res. 42 Pages: 527-535
  • Peer Reviewed
• [Presentation] 歯根膜細胞分化におけるcementum protein(CEMP)1発現と機能解析 (2010)
  • Author(s): 小牧基浩、岩崎剣吾, 森田育男
  • Organizer: 第31回日本炎症再生医学会
  • Place of Presentation: 京王プラザホテル、東京
• [Presentation] 歯根膜幹細胞のpericyteとしての機能解析 (2010)
  • Author(s): 岩崎剣吾, 木村康之, 小牧基浩
  • Organizer: 第53回秋期日本歯周病学会
  • Place of Presentation: サンポートホール高松、香川県
• [Presentation] 歯根膜幹細胞のpericyteとしての機能解析 (2010)
  • Author(s): 岩崎剣吾, 木村康之, 小牧基浩
  • Organizer: 第53回秋期日本歯周病学会
  • Place of Presentation: 香川県、サンポートホール高松
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるCP-23発現の検討 (2009)
  • Author(s): 小牧基浩、森田育男、和泉雄一、安部まゆみ
  • Organizer: 第51回春期日本歯周病学会
  • Place of Presentation: 岡山コンベンションセンター、岡山
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるCP-23発現の検討 (2009)
  • Author(s): 小牧基浩, 他
  • Organizer: 日本歯周病学会
  • Place of Presentation: 岡山コンベンションセンター
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるCP-23発現の検討 (2008)
  • Author(s): 小牧基浩、津川順一、中浜健一、森田育男
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場、大阪
• [Presentation] 印刷技術を用いて羊膜に転写した骨芽細胞による骨再生療法 (2008)
  • Author(s): 津川順一、小牧基浩、天笠光雄、森田育男
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場、大阪
• [Presentation] Cementum protein-23 expression in osteoblastic differentiation of periodontal ligament cells (2008)
  • Author(s): Motohiro Komaki, Kengo Iwasaki, A. Sampath Narayanan, Higinio Arzate, Yuichi Izumi, Mayumi Abe
  • Organizer: 94th Annual Meeting of the American Academy of Periodontology's September 7-9^<th>
  • Place of Presentation: The Washington State Convention & Trade Center, Seattle, WA, USA
• [Presentation] 歯根膜細胞の骨芽細胞分化におけるCP-23発現の検討 (2008)
  • Author(s): Komaki, M
  • Organizer: 第26回目本骨代謝学会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] Cementum protein-23 expression in OSteoblast ic differentiation of periodontal ligament cells (2008)
  • Author(s): Komaki, M.
  • Organizer: 94th Amer ican Academy of Per iodontology
  • Place of Presentation: Washington state convent ion andtrade center (Seattle, USA)