A study on the effects of products from non-hemocyte cells activated by crosslinking HLA-II molecules on Th-cell differentiation in periodontal diseases

• Project/Area Number: 20592443
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Hyogo College of Medicine
• Principal Investigator: OHYAMA Hideki Hyogo College of Medicine, 医学部, 准教授 (90280685)
• Co-Investigator(Kenkyū-buntansha): TERADA Nobuyuki 兵庫医科大学, 医学部, 教授 (50150339) ; NAKASHO Keiji 兵庫医科大学, 医学部, 教授 (00217712) ; YAMADA Naoko 兵庫医科大学, 医学部, 講師 (10319858) ; YAMANEGI Koji 兵庫医科大学, 医学部, 講師 (00434944) ; URADE Masahiro 兵庫医科大学, 医学部, 教授 (70104883)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 歯肉線維芽細胞 / 樹状細胞 / ヘルパーT細胞 / クラスII HLA分子 / サイトカイン / クテスII HLA分子 / クラスIIHLA分子

Research Abstract

In the study to examine the effects of products from human gingival fibroblasts (HGF) activated by cross-linking HLA-II molecules on Th-cell response and differentiation, we found that Th cells exhibited a Th2-shifted phenotype after mixed lymphocyte reaction culture between naive T cells and allogeneic DCs in the presence of culture supernatant from HGF stimulated via HLA-DQ molecules. The regulation of Th2 polarization in Th-cells could be partially attributed to the activation of DCs being affected by PGE_2 produced from HGF stimulated via HLA-DQ.

Research Products

• [Journal Article] Adipocyte-macrophage interaction may mediate LPS-induced low-grade inflammation : potential link with metabolic complications. (2011)
  • Author(s): Nakarai H., Yamashita A., Nagayasu S., Iwashita M., Kumamoto, S., Ohyama H., Hata M., Soga Y., Kushiyama A., Asano T., Abiko Y., Nishimura F.
  • Journal Title: Innate Immunity (in press)
  • Peer Reviewed
• [Journal Article] Adipocyte-macrophage interaction may mediate LPS-induced low-grade inflammation : potential link with metabolic complications. (2011)
  • Author(s): Hideo Nakarai
  • Journal Title: Innate Immunity Volume: (in press)
  • Peer Reviewed
• [Journal Article] Fibroblasts stimulated via HLA-II molecules produce prostaglandin E2 and regulate cytokine production from helper T cells. (2010)
  • Author(s): Kato-Kogoe N., Ohyama H., Nishimura F., Meguro M., Yoshizawa S., Okada Y., Nakasho K., Yamanegi K., Yamada N., Hata M., Higashi T., Terada N., Matsushita S.
  • Journal Title: Lab.Invest. 90 Pages: 1747-1756
  • Peer Reviewed
• [Journal Article] T細胞応答性から捉えた歯周病に対する疾患感受性の個体差に関する免疫遺伝学的研究 (2010)
  • Author(s): 大山秀樹
  • Journal Title: 日本歯周病学会会(日本歯周病学会学術賞受賞記念 総説)誌 52 Pages: 14-23
  • NAID: 10027096058
• [Journal Article] Clinical and Immunological Assessment of Periodontal Disease in Japanese Leprosy Patients. (2010)
  • Author(s): Ohyama H., Hongyo H., Shimizu N., Shimizu Y., Nishimura F., Nakagawa M., Arai H., Kato-Kogoe N., Terada N., Nagai A., Takashiba S., Kurihara H., Nomura Y., Murayama Y.
  • Journal Title: Jpn.J.Infect.Dis. 63 Pages: 427-432
  • NAID: 40017386094
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Yamanegi K., Yamane J., Kobayashi K., Kato-Kogoe N., Ohyama H., Nakasho K., Yamada N., Hata M., Nishioka T., Fukunaga S., Futani H., Okamura H., Terada N.
  • Journal Title: Oncology Reports 24 Pages: 1621-1627
  • Peer Reviewed
• [Journal Article] Fibroblasts stimulated via HLA-II molecules produce prostaglandin E_2 and regulate cytokine production from helper T cells. (2010)
  • Author(s): Nahoko Kato-Kogoe
  • Journal Title: Laboratory Investigation Volume: 90 Pages: 1747-1756
  • Peer Reviewed
• [Journal Article] Clinical and immunological assessment of periodontal disease in Japanese leprosy patients. (2010)
  • Author(s): Hideki Ohyama
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 63 Pages: 427-432
  • NAID: 40017386094
  • Peer Reviewed
• [Journal Article] Sodium valproate, a histone deacetylase inhibitor, augments the expression of cell-surface NKG2D ligand, MICA and B, without increasing their soluble forms to enhance susceptibility of human osteosarcoma cells to NK cell-mediated cytotoxicity. (2010)
  • Author(s): Koji Yamanegi
  • Journal Title: Oncology Reports Volume: 24 Pages: 1621-1627
  • Peer Reviewed
• [Journal Article] T細胞応答性から捉えた歯周病に対する疾患感受性の個体差に関する免疫遺伝学的研究. (2010)
  • Author(s): 大山秀樹
  • Journal Title: 日本歯科周病学会会誌 52 Pages: 14-23
  • NAID: 10027096058
• [Journal Article] HLA-II-induced cytokines from human gingical fibroblasts promotes proliferation of human umbilical vein endothelial (HUVEC) cells : potential association with enhanced angiogenesis in chronic periodontal inflammation. (2009)
  • Author(s): Okada Y., Meguro M., Ohyama H., Yoshizawa S., Takeuchi-Hatanaka K., Kato N., Matsushita S., Takashiba S., Nishimura F.
  • Journal Title: J.Periodont.Res. 44 Pages: 103-109
  • Peer Reviewed
• [Journal Article] The involvement of IL-23 and Th17 pathway in periodontitis. (2009)
  • Author(s): Ohyama H., Kato-Kogoe N., Kuhara A., Nishimura F., Nakasho K., Yamanegi K., Yamada N., Hata M., Yamane J., Terada N.
  • Journal Title: J.Dent.Res. 88 Pages: 633-638
  • NAID: 130004570957
  • Peer Reviewed
• [Journal Article] Genetic Risk Factor for Periodontitis in a Japanese Population. (2009)
  • Author(s): Kobayashi T., Nagata T., Murakami S., Takashiba S., Kurihara H., Izumi Y., Numabe Y., Watanabe H., Kataoka M., Nagai A., Hayashi J., Ohyama H., Okamatsu Y., Inagaki Y., Tai H., Yoshie H.
  • Journal Title: J.Dent.Res. 88 Pages: 1137-1141
  • Peer Reviewed
• [Journal Article] An unusual autopsy case of pyogenic liver abscess caused by periodontal bacteria. (2009)
  • Author(s): Ohyama H., Nakasho K., Yamanegi K., Noiri Y., Kuhara A., Kato-Kogoe K., Yamada N., Hata M., Nishimura F., Ebisu S., Terada N.
  • Journal Title: Jpn.J.Infect.Dis. 62 Pages: 381-383
  • NAID: 40016750210
  • Peer Reviewed
• [Journal Article] HLA-II-induced cytokines from human gingival fibroblasts promotes proliferation of human umbilical vein endothelial(HUVEC)cells : potential association with enhanced angiogenesis in chronic periodontal inflammation. (2009)
  • Author(s): Yuka Okada
  • Journal Title: Journal of Periodontal Research 44 Pages: 103-109
  • Peer Reviewed
• [Journal Article] The involvement of IL-23and Th17 pathway in periodontitis. (2009)
  • Author(s): Hideki Ohyama
  • Journal Title: Journal of Dental Research 88 Pages: 633-638
  • Peer Reviewed
• [Journal Article] The involvement of IL-23 and Th17 pathway in periodontitis. (2009)
  • Author(s): Hideki Ohyama
  • Journal Title: Journal of Dental Research (in press)
  • NAID: 130004570957
  • Peer Reviewed
• [Journal Article] HLA-II-induced cytokines from human gingical fibroblasts promotes proliferation of human umbilical vein endothelial (HUVEC) cells : potential association with enhanced angiogenesis in chronic periodontal inflammation. (2009)
  • Author(s): Yuka Okada
  • Journal Title: Journal of Periodontal Research (in press)
  • Peer Reviewed
• [Journal Article] Enhanced suppression of pulmonary metastasis of malignant melanoma cells by combined administration of alpha-galactosylceramide and interleukin-18. (2008)
  • Author(s): Nishio S., Yamada N., Ohyama H., Yamanegi K., Nakasho K., Hata M., Nakamura Y., Fukunaga S., Futani H., Yoshiya S., Ueda H., Taniguchi M., Okamura H., Terada N.
  • Journal Title: Cancer Sci. 99 Pages: 113-120
  • Peer Reviewed
• [Journal Article] Polymorphisms in the 5' flanking region of IL12RB2 are associated with susceptibility to periodontal diseases in the Japanese population. (2008)
  • Author(s): Takeuchi-Hatanaka K., Ohyama H., Nishimura F., Kato-Kogoe N., Soga Y., Matsushita S., Nakasho K., Yamanegi K., Yamada N., Terada N., Takashiba S.
  • Journal Title: J.Clin.Periodontol. 35 Pages: 317-323
  • Peer Reviewed
• [Journal Article] Transdifferentiation into biliary ductular cells of hepatocytes transplanted into the spleen. (2008)
  • Author(s): Watanabe H., Hata M., Terada N., Ueda H., Yamada N., Yamanegi K., Ohyama H., Kakihana M., Okamura H., Nakasho K.
  • Journal Title: Pathology 40 Pages: 272-276
  • Peer Reviewed
• [Journal Article] Differential effect of polymorphisms in the 5' flanking region of IL12RB2 on NK- and T-cll activity. (2008)
  • Author(s): Ohyama H., Kato-Kogoe N., Nishimura F., Takeuchi-Hatanaka K., Matsushita S., Yamanegi K., Yamada N., Hata M., Nakasho K., Terada N.
  • Journal Title: J.Interferon Cytokine Res. 28 Pages: 563-569
  • Peer Reviewed
• [Journal Article] Histochemical demonstration of aluminum and iron deposition in pulmonary bony tissues in three cases of diffuse pulmonary ossification. (2008)
  • Author(s): Ohtsuki Y., Yamanaka A., Ohyama H., Yamada E., Terada N., Fujita J., Lee G.H., Furihata M.
  • Journal Title: Histol.Histopathol. 23 Pages: 137-141
  • Peer Reviewed
• [Journal Article] Role of human non-invariant NKT lymphocytes in the maintenance of type 2 T helper environment during pregnancy. (2008)
  • Author(s): Uemura Y., Suzuki M., Liu T.Y., Narita Y., Hirata S., Ohyama H., Ishihara O., Matsushita S.
  • Journal Title: Int.Immunol. 20 Pages: 405-412
  • NAID: 10021239996
  • Peer Reviewed
• [Journal Article] Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. (2008)
  • Author(s): Liu T.Y., Uemura Y., Suzuki M., Narita Y., Hirata S., Ohyama H., Ishihara O., Matsushita S.
  • Journal Title: Eur.J.Immunol. 38 Pages: 1012-1023
  • Peer Reviewed
• [Journal Article] Kaposi's sarcoma-associated herpesvirus ORF57 functions as a viral splicing factor and promotes the expression of intron-containing viral lytic genes in spliceosome-mediated RNA spicing. (2008)
  • Author(s): Majerciak V., Yamanegi K., Allemand E., Kruhlak M., Krainer A.R., Zheng Z.M.
  • Journal Title: J.Virol. 82 Pages: 792-2801
  • Peer Reviewed
• [Journal Article] Differential effects of polymorphisms in the 5' flanking region of IL12RB2 on NK and T cell activity. (2008)
  • Author(s): Hideki Ohyama
  • Journal Title: Journal of Interferon & Cytokine Research 28 Pages: 563-569
  • Peer Reviewed
• [Presentation] 短期集中的な口腔衛生管理により重度白血病性歯肉炎の軽快を見た急性骨髄性白血病患者の1症例 (2010)
  • Author(s): 蔵下舞,大山秀樹,木崎久美子,橋谷進
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-15
• [Presentation] T細胞応答性から捉えた歯周病に対する疾患感受性の個体差に関する免疫遺伝学的研究(学会学術賞受賞記念講演) (2010)
  • Author(s): 大山秀樹
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-14
• [Presentation] 造血幹細胞移植の予後に関連する歯周疾患因子について (2010)
  • Author(s): 橋谷進,木崎久美子,蔵下舞,大山秀樹
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-14
• [Presentation] 学会学術賞受賞記念講演:T細胞応答性から捉えた歯周病に対する疾患感受性の個体差に関する免疫遺伝学的研究 (2010)
  • Author(s): 大山秀樹
  • Organizer: 第53回春季日本歯周病学会学術大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-14
• [Presentation] IL-22がヒト歯根膜細胞の石灰化ノジュール形成に及ぼす影響 (2010)
  • Author(s): 大山秀樹,小越菜保子,西岡稔浩,山根木康嗣,山田直子,秦正樹,中正恵二,寺田信行
  • Organizer: 第99回日本病理学会総会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-29
• [Presentation] 歯周病細菌感染に起因したと考えられる化膿性肝膿瘍の1剖検例 (2009)
  • Author(s): 大山秀樹,野杁由一郎,小越菜保子,西村英紀,恵比須繁之
  • Organizer: 第130回日本歯科保存学会2009年度春季学術大会
  • Place of Presentation: 札幌
  • Year and Date: 2009-06-12
• [Presentation] 歯周病病態形成におけるIL-23/IL-17産生系の関与 (2009)
  • Author(s): 大山秀樹,小越菜保子,西村英紀,山根木康嗣,橋谷進,木崎久美子
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-15
• [Presentation] 当院妊婦母親教室参加者における歯周病に対する意識調査 (2009)
  • Author(s): 橋谷進,木崎久美子,大山秀樹
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2009-05-15
• [Presentation] IL12RB2転写制御領域の多型はT細胞とNK細胞との間で異なった影響を及ぼす (2009)
  • Author(s): 大山秀樹,小越菜保子,西岡稔浩,中正恵二,山根木康嗣,山田直子,秦正樹,山根純子,寺田信行
  • Organizer: 第96回日本病理学会総会
  • Place of Presentation: 京都
  • Year and Date: 2009-05-03
• [Presentation] クラスII HLA分子を介した刺激を受けた歯肉線維芽細胞によるTh細胞応答性の制御 (2008)
  • Author(s): 大山秀樹,小越菜保子,目黒道生,吉澤さゆり,岡田祐佳,西村英紀
  • Organizer: 第129回日本歯科保存学会秋季学術大会
  • Place of Presentation: 富山
  • Year and Date: 2008-11-07
• [Presentation] 全身病理における歯周病医学の位置づけ (2008)
  • Author(s): 大山秀樹
  • Organizer: 東北大学歯学部同窓会サマーセミナー
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-07
• [Presentation] 歯周病-感染性疾患として全身に及ぼす影響- (2008)
  • Author(s): 大山秀樹
  • Organizer: 第42回日本病理学会近畿支部学術集会
  • Place of Presentation: 西宮
  • Year and Date: 2008-09-06
• [Presentation] 脾臓に移植された肝細胞の胆管上皮細胞への分化 (2008)
  • Author(s): 秦正樹,渡邉博文,大山秀樹,山田直子,山根木康嗣,小越菜保子,山根純子,中正恵二,寺田信行
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] 歯周病細菌感染に起因したと考えられる化膿性肝膿瘍の1剖検例 (2008)
  • Author(s): 鷲尾輝明,大山秀樹,中正恵二,山根木康嗣,久原彩子,小越菜保子,山田直子,秦正樹,山根純子,野杁由一郎,恵比須繁之,寺田信行
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] ヒト骨肉腫細胞の免疫療法抵抗性に対するhistone deacetylase inhibitorの効果 (2008)
  • Author(s): 山根木康嗣,山根純子,秦正樹,大山秀樹,山田直子,小越菜保子,中正恵二,寺田信行
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Presentation] IL12RB2転写制御領域の遺伝子多型が侵襲性歯周炎に対する疾患感受性の個体差に及ぼす影響 (2008)
  • Author(s): 大山秀樹,畑中加珠,小越菜保子,西村英紀,曽我賢彦,中正恵二,山根木康嗣,山田直子,秦正樹,山根純子,高柴正悟,寺田信行
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-15
• [Book] Leprosy-Science working towards dignity.(Ed : Makino M, Matsuoka M, Goto M, Hatano K)(Chapter 8. Immunogenetics.) (2011)
  • Author(s): 大山秀樹
  • Publisher: Tokai University Press, Kanagawa
• [Book] Leprosy-Science working towards dignity (2011)
  • Author(s): Hideki Ohyama
  • Publisher: Tokai University Press
• [Book] Leprosy (2010)
  • Author(s): Hideki Ohyama
  • Publisher: Tokai University Press(in press)
• [Book] エンド難症例・メカニズムと臨床応用(恵比須繁之 編)(Chapter5エンド治療に関連するトピック 歯内疾患と全身の健康) (2009)
  • Author(s): 大山秀樹,小越菜保子
  • Publisher: 医歯薬出版
• [Patent(Industrial Property Rights)] IFN-・低産生関連IL-12Rβ2遺伝子プロモーター領域の多型とその検出方法 (2010)
  • Inventor(s): 緒方是嗣,山本卓志,松下祥,大山秀樹
  • Industrial Property Rights Holder: 株式会社島津製作所,松下祥,大山秀樹
  • Acquisition Date: 2010-12-24
• [Patent(Industrial Property Rights)] IFN-γ低産生関連IL-12R β2遺伝子プロモーター領域の多型とその検出方法 (2010)
  • Inventor(s): 緒方是嗣, 山本卓志, 松下祥, 大山秀樹
  • Industrial Property Rights Holder: 緒方是嗣, 山本卓志, 松下祥, 大山秀樹
  • Acquisition Date: 2010-12-24