Proteomics of Porphyromonas gingivalis within a model oral microbial community

• Project/Area Number: 20592453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Osaka University
• Principal Investigator: KUBONIWA Masae Osaka University, 大学院・歯学研究科, 助教 (00303983)
• Co-Investigator(Kenkyū-buntansha): MAEDA Kazuhiko 大阪大学, 大学院・歯学研究科, 助教 (00346165) ; SEKINE Shinichi 大阪大学, 大学院・歯学研究科, 助教 (70506344)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: バイオフイルム / プロテオミクス / 歯周病原性細菌 / 代謝経路 / ビタミンB / 歯周病 / プロテオーム / porphyromonas gingivalis / Fusobacterium nucleatum / Streptococcus gordonii / Porphyromonas gingivalis

Research Abstract

P.gingivalis can assemble into a heterotypic community with a middle colonizer F.nucleatum and an early colonizer S.gordonii, and that a community lifestyle provides physiologic support for P.gingivalis. 1156 P.gingivalis proteins, including 403 down-regulated and 89 up-regulated proteins, were detected qualitatively during comparison of the three species model community with P.gingivalis incubated alone under the same conditions. Significant decreases were seen in proteins involved in thiamine, cobalamin, and pyrimidine synthesis. These results indicate that a community lifestyle provides physiologic support for P.gingivalis.

Research Products

• [Journal Article] Subgingival biofilm formation (2010)
  • Author(s): Kuboniwa, M., R.J. Lamont.
  • Journal Title: Periodontol 2000 52(1) Pages: 38-52
  • Peer Reviewed
• [Journal Article] Subgingival biofilm formation (2010)
  • Author(s): Kuboniwa, M.
  • Journal Title: Periodontology 2000 52 Pages: 38-52
  • Peer Reviewed
• [Journal Article] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): Kuboniwa, M., E.L. Hendrickson, Q. Xia, T. Wang, H. Xie, M. Hackett, R.J. Lamont
  • Journal Title: BMC Microbiol 19(9) Pages: 98-98
  • Peer Reviewed
• [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations (2009)
  • Author(s): Kuboniwa, M., A. Amano, H. Inaba, E. Hashino, S. Shizukuishi.
  • Journal Title: Oral Microbiol Immunol 24(3) Pages: 260-3
  • Peer Reviewed
• [Journal Article] Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis (2009)
  • Author(s): Kuboniwa, M., A. Amano, E. Hashino, Y. Yamamoto, H. Inaba, N. Hamada, K. Nakayama, G.D. Tribble, R.J. Lamont, S. Shizukuishi.
  • Journal Title: BMC Microbiol 26(9) Pages: 105-105
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis invades human trophoblasts and inhibits proliferation by inducing G1 arrest and apoptosis (2009)
  • Author(s): Inaba, H., M. Kuboniwa, B. Bainbridge, O. Yilmaz, J. Katz, K.T. Shiverick, A. Amano, R.J. Lamont.
  • Journal Title: Cell Microbiol 11(10) Pages: 1517-32
  • Peer Reviewed
• [Journal Article] Identification of binding domain of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase for Porphyromonas gingivalis major fimbriae (2009)
  • Author(s): Nagata, H., M. Iwasaki, K. Maeda, M. Kuboniwa, E. Hashino, M. Toe, N. Minamino, H. Kuwahara, S. Shizukuishi.
  • Journal Title: Infect Immun 77(11) Pages: 5130-8
  • Peer Reviewed
• [Journal Article] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): Kuboniwa, M.
  • Journal Title: BMC Microbiology 9 Pages: 98-98
  • Peer Reviewed
• [Journal Article] Distinct roles of long/short fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis (2009)
  • Author(s): Kuboniwa, M.
  • Journal Title: BMC Microbiology 9 Pages: 105-105
  • Peer Reviewed
• [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations (2009)
  • Author(s): Kuboniwa, M.
  • Journal Title: Oral Microbiology and Immunology 24 Pages: 260-263
  • Peer Reviewed
• [Journal Article] Porphyromonas gingivalis invades human trophoblasts and inhibits proliferation by inducing G1 arrest and apoptosis (2009)
  • Author(s): Inaba, H.
  • Journal Title: Cellular Microbiology 11 Pages: 1517-1532
  • Peer Reviewed
• [Journal Article] Identification of binding domain of Streptococcus oralis glyceraldehyde-3-phosphate dehydrogenase for Porphyromonas gingivalis major fimbriae (2009)
  • Author(s): Nagata, H.
  • Journal Title: Infection and Immunity 77 Pages: 5130-5138
  • Peer Reviewed
• [Journal Article] Homotypic biofilm structure of Porphyromonas gingivalis is affected by FimA type variations (2009)
  • Author(s): Kuboniwa, M.
  • Journal Title: Oral Microbiology and Immunology 24(in press)
  • Peer Reviewed
• [Journal Article] gingivalis accelerates gingival epithelial cell progression through the cell cycle (2008)
  • Author(s): Kuboniwa, M., Y. Hasegawa, S. Mao, S. Shizukuishi, A. Amano, R.J. Lamont, O. Yilmaz. P.
  • Journal Title: Microbes Infect 10(2) Pages: 122-8
  • Peer Reviewed
• [Journal Article] Heterogenic virulence and related factors among clinical isolates of Porphyromonas gingivalis with type II fimbriae (2008)
  • Author(s): Inaba, H., K. Nakano, T. Kato, R. Nomura, S. Kawai, M. Kuboniwa, K. Ishihara, T. Ooshima, A. Amano.
  • Journal Title: Oral Microbiol Immunol 23(1) Pages: 29-35
  • Peer Reviewed
• [Journal Article] Effect of eucalyptus extract chewing gum on periodontal health: a double-masked, randomized trial (2008)
  • Author(s): Nagata, H., Y. Inagaki, M. Tanaka, M. Ojima, K. Kataoka, M. Kuboniwa, N. Nishida, K. Shimizu, K. Osawa, S. Shizukuishi.
  • Journal Title: J Periodontol 79(8) Pages: 1378-85
  • Peer Reviewed
• [Journal Article] A Porphyromonas gingivalis tyrosine phosphatase is a multifunctional regulator of virulence attributes (2008)
  • Author(s): Maeda, K., G.D. Tribble, C.M. Tucker, C. Anaya, S. Shizukuishi, J.P. Lewis, D.R. Demuth, R.J. Lamont.
  • Journal Title: Mol Microbiol 69(5) Pages: 1153-64
  • Peer Reviewed
• [Journal Article] A novel adenovirus expressing Flt3 ligand enhances mucosal immunity by inducing mature nasopharyngeal-associated lymphoreticular tissue dendritic cell migration (2008)
  • Author(s): Sekine, S., K. Kataoka, Y. Fukuyama, Y. Adachi, J. Davydova, M. Yamamoto, R. Kobayashi, K. Fujihashi, H. Suzuki, D.T. Curiel, S. Shizukuishi, J.R. McGhee, K. Fujihashi.
  • Journal Title: J Immunol 180(12) Pages: 8126-34
  • Peer Reviewed
• [Journal Article] Association between involuntary smoking and salivary markers relatedto periodontitis : a 2-year longitudinal study (2008)
  • Author(s): Nishida, N.
  • Journal Title: Journal of Periodontology 79 Pages: 2233-2240
  • Peer Reviewed
• [Journal Article] Effect of eucalyptus extract chewing gum on periodontal health : a double-masked, randomized trial (2008)
  • Author(s): Nagata, H.
  • Journal Title: Journal of Periodontology 79 Pages: 1378-1385
  • Peer Reviewed
• [Presentation] デンタルバイオフィルム形成に及ぼすStreptococcus oralis GAPDHペプチドの影響 (2009)
  • Author(s): 岩崎未央, 橋野恵衣, 東江正裕, 永田英樹, 久保庭雅恵, 雫石聰
  • Organizer: 第20回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 広島市
  • Year and Date: 2009-06-21
• [Presentation] エリスリトールは歯周病原性細菌の揮発性硫化物産生を抑制する (2009)
  • Author(s): 橋野恵衣, 久保庭雅恵, 東江正裕, 関根伸一, 田中宗雄, 雫石聰
  • Organizer: 第20回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 広島市
  • Year and Date: 2009-06-21
• [Presentation] エリスリトールは歯周病原性細菌の揮発性硫化物産生を抑制する (2009)
  • Author(s): 橋野恵衣
  • Organizer: 第20回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 広島国際会議場大会議室「ダリア」, 広島
  • Year and Date: 2009-06-21
• [Presentation] デンタルバイオフィルム形成に及ぼす Streptococcus oralis GAPDHペプチドの影響 (2009)
  • Author(s): 岩崎未央
  • Organizer: 第20回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 広島国際会議場大会議室「ダリア」, 広島
  • Year and Date: 2009-06-21
• [Presentation] Proteomics of Porphyromonas gingivalis within a model oral microbial community (2009)
  • Author(s): Kuboniwa, M., Hendrickson, E.L., Xie, H., Hashino, E., Shizukuishi, S., Hackett, M., Lamont, R.J.
  • Organizer: The 2009 Gordon Research Conference on Periodontal Disease
  • Place of Presentation: Colby-Sawyer College, NH, USA
• [Presentation] エリスリトールによるPorphyromonas gingivalis発現タンパク質の変化に関する網羅的解析 (2009)
  • Author(s): 橋野恵衣, 久保庭雅恵, 山本裕美子, 末松和博, 長洋, 雫石聰
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 岡山市
• [Presentation] Streptococcus oralis GAPDH peptide inhibits Porphyromonas gingivalis biofilm formation (2009)
  • Author(s): Nagata, H., Iwasaki, M., Kuboniwa, M., Maeda, K., Sekine, S., Shizukuishi, S.
  • Organizer: The 87th General Session & Exhibition of the IADR
  • Place of Presentation: Maiami, FL, USA
• [Presentation] エリスリトールによる Porphyromonas gingivalis 発現タンパク質の変化に関する網羅的解析 (2009)
  • Author(s): 橋野恵衣
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 岡山コンベンションセンター (ママカリフォーラム), 岡山
• [Presentation] 気管切開チュフブ装着患者の下気道より検出される歯周病細菌の基礎的解析 (2008)
  • Author(s): 江間弘子
  • Organizer: 第4回ホロニクスグループ学術集会
  • Place of Presentation: 大阪市
  • Year and Date: 2008-12-14
• [Presentation] Porphyromonas gingivalis線毛への口腔レンサ球菌菌体表層glyceraldehyde-3-phosphate dehydrogenaseの結合部位の解析 (2008)
  • Author(s): 岩崎未央, 永田英樹, 前田和彦, 久保庭雅恵, 橋野恵衣, 雫石聰
  • Organizer: 大阪大学歯学会第106回例会
  • Place of Presentation: 吹田市
  • Year and Date: 2008-07-17
• [Presentation] 人工呼吸器装着患者の下気道より検出される歯周病細菌の基礎的解析 (2008)
  • Author(s): 上月弘子
  • Organizer: 第19回日本口腔衛生学会近畿・中国・四国地方会総会
  • Place of Presentation: 徳島市
  • Year and Date: 2008-06-22
• [Presentation] Distinct roles of major/minor fimbriae and gingipains in homotypic biofilm development by Porphyromonas gingivalis. Biofilms III (2008)
  • Author(s): Kuboniwa, M., Amano, A., Hashino, E., Shizukuishi, S.
  • Organizer: 3rd International Conference
  • Place of Presentation: Munich, Germany
• [Presentation] Porphyromonas gingivalisのバイオフィルム形成に及ぼすGAPDHペプチドの阻害効果 (2008)
  • Author(s): 岩崎未央, 永田英樹, 久保庭雅恵, 前田和彦, 橋野恵衣, 雫石聰
  • Organizer: 第57回日本口腔衛生学会・総会
  • Place of Presentation: さいたま市
• [Presentation] Role of P. gingivalis PtpA tyrosine phosphatase in exopolysaccharide production (2008)
  • Author(s): Tucker, C.M., Maeda, K., Tribble, G.D., Hagerty D., Bainbridge B., Lamont R.J.
  • Organizer: 86th General session & exhibition of the IADR
  • Place of Presentation: Toronto, Canada
• [Presentation] Role of Streptococcus gordonii Cbe protein in periodontopathic biofilm development (2008)
  • Author(s): Kuboniwa, M., Hashino, E., Yamamoto, Y., Lamont, R.J., Shizukuishi, S.
  • Organizer: 86th General session & exhibition of the IADR
  • Place of Presentation: Toronto, Canada
• [Presentation] Role of Streptococcus gordonii Cbe protein in periodontopathicbiofilm development. (2008)
  • Author(s): Kuboniwa, M.
  • Organizer: 86th General session & exhibition of the IADR
  • Place of Presentation: Toronto, Canada
• [Presentation] Distinct roles of major/minor fimbriae and gingipains in homotypicbiofilm development by Porphyromonas gingivalis (2008)
  • Author(s): Kuboniwa, M.
  • Organizer: Biofilms III : 3^<rd> International Conference
  • Place of Presentation: Munich, Germany
• [Book] Heterotypic Streptococcus gordonii-Porphyromonas gingivalis communities: formation, gene regulation and development (2010)
  • Author(s): Kuboniwa, M., R. J. Lamont Chapter
  • Publisher: Genomic inquiries into oral bacterial communities(in press)
• [Book] Chapter "Heterotypic Streptococcus gordonii-Porphyromonas gingivalis communities : formation, gene regulation and development", in Genomic Inquiries Into Oral Bacterial Communities (ed. Paul E. Kolenbrander)
  • Author(s): Kuboniwa, M.
  • Publisher: ASM press(in press)