Investigation of effect of soluble factors from adipose tissue on periodontitis, and development of prophylactic approach with these regulation

• Project/Area Number: 20592457
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: AKIFUSA Sumio Kyushu University, 歯学研究院, 准教授 (40295861)
• Co-Investigator(Kenkyū-buntansha): YAMASHITA Yoshihisa 九州大学, 大学院・歯学研究院, 教授 (20192403)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: メタボリックシンドローム / 歯周疾患 / 肥満

Research Abstract

We examined the effect of adiponectin, one of adipokine derived from mature adipocyte, on activity of macrophage, and found that administration of adiponectin reduced the lipopolysaccharide-induced inflammatory response. On the other hand, stimulation with adiponectin alone induced cytokine secretion, generation of reactive oxygen species and nitric oxide, followed by apoptotic cell death, in macrophage.

Research Products

• [Journal Article] Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species. (2011)
  • Author(s): Kamio N, Akifusa S, Yamashita Y
  • Journal Title: Free Radic Res. vol.45 Pages: 336-341
• [Journal Article] Diacylglycerol kinase alpha regulates globular adiponectin-induced reactive oxygen species. (2011)
  • Author(s): Kamio N, Akifusa S, Yamashita Y.
  • Journal Title: Free Radical Research Volume: 45 Pages: 336-341
  • Peer Reviewed
• [Journal Article] Involvement of the JAK-STAT pathway and SOCS3 in the regulation of adiponectin-generated reactive oxygen species in murine macrophage RAW 264 cells. (2010)
  • Author(s): Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, Nonaka K, Yamashita Y
  • Journal Title: J Cell Biochem. vol.111 Pages: 597-606
  • Peer Reviewed
• [Journal Article] Expression levels of adiponectin receptors and periodontitis. (2010)
  • Author(s): Yamaguchi N, Hamachi T, Kamio N, Akifusa S
  • Journal Title: J Periodont Res. vol.45 Pages: 296-300
  • Peer Reviewed
• [Journal Article] Involvement of mTOR in globular adiponectin-induced generation of reactive oxygen species. (2010)
  • Author(s): Fujimoto A, Akifusa S, Kamio N, Hirofuji T, Nonaka K, Yamashita Y
  • Journal Title: Free Rad Res. vol.44 Pages: 128-134
  • Peer Reviewed
• [Journal Article] Expression levels of adiponectin receptors and periodontitis. (2010)
  • Author(s): Yamaguchi N, Hamachi T, Kamio N, Akifusa S, et al.
  • Journal Title: Journal of Periodontal Research Volume: 45 Pages: 296-300
  • Peer Reviewed
• [Journal Article] Relationship between obesity and physical fitness and periodontitis. (2010)
  • Author(s): Shimazaki Y, Egami Y, Matsubara T, Koike G, Akifusa S, et al.
  • Journal Title: Journal of Periodontology Volume: 81 Pages: 1124-1131
  • Peer Reviewed
• [Journal Article] Involvement of the JAK-STAT pathway and SOCS3 in the regulation of adiponectin-generated reactive oxygen species in murine macrophage RAW 264 cells. (2010)
  • Author(s): Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, et al.
  • Journal Title: Journal of Cell Biochemistry Volume: 111 Pages: 597-606
  • Peer Reviewed
• [Journal Article] Anti-inflammatory activity of a globular adiponectin function on RAW 264 cells stimulated by lipopolysaccharide from Aggregatibacter actinomycetemcomitans. (2009)
  • Author(s): Kamio N, Akifusa S, Yamaguchi N, Nonaka K, Yamashita Y
  • Journal Title: FEMS Immunol Med Microbiol. vol.56 Pages: 241-247
  • Peer Reviewed
• [Journal Article] Globular adiponectin-induced RAW 264 apoptosis is regulated by reactive oxygen species-dependent pathway involving Bcl-2. (2009)
  • Author(s): Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, Nishihara T, Yamashita Y
  • Journal Title: Free Radic Biol Med vol.46 Pages: 1308-1316
  • Peer Reviewed
• [Journal Article] Involvement of Ca^<2+> in globular adiponectin-induced reactive oxygen species. (2009)
  • Author(s): Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, Yamashita Y
  • Journal Title: Biochem. Biophys. Res.Commun. vol.381 Pages: 649-653
  • Peer Reviewed
• [Journal Article] Anti-inflammatory activity of a globular adiponectin function on RAW 264 cells stimulated by lipopolysaccharide from Aggregatibacter actinomy cetemcomitans (2009)
  • Author(s): Kamio N., et al.
  • Journal Title: FEMS Immunology and Medical Microbiology 56 Pages: 241-241
  • Peer Reviewed
• [Journal Article] Globular adiponectin-induced RAW 264 apoptosis is regulated by a reactive oxygen species-dependent pathway involving Bcl-2 (2009)
  • Author(s): Akifusa S., et al.
  • Journal Title: Free Radical Biology and Medicine 46 Pages: 1308-1308
  • Peer Reviewed
• [Journal Article] Involvement of Ca^<2+> in globular adiponectin-induced reactive oxygen species (2009)
  • Author(s): Akifusa S., et al.
  • Journal Title: Biochemical and Biophysical Research Communications 381 Pages: 649-649
  • Peer Reviewed
• [Journal Article] Regulation of Globular Adiponectin-Induced Apoptosis by Reactive Oxygen/Nitrogen Species in RAW264 Macrophages. (2008)
  • Author(s): Akifusa S, Kamio N, Shimazaki Y, Yamaguchi N, Yamashita Y
  • Journal Title: Free Radic Biol Med vol.45 Pages: 1326-1339
  • Peer Reviewed
• [Journal Article] Induction of granulocyte colony-stimulating factor by globular adiponectin via the MEK-ERK pathway. (2008)
  • Author(s): Kamio N, Akifusa S, Yamaguchi N, Yamashita Y
  • Journal Title: Mol Cell Endocrinol. vol.292 Pages: 20-25
  • Peer Reviewed
• [Journal Article] Regulation of globular adiponectin-induced apoptosis by reactive oxygen/nitrogen species in RAW264 macrophages (2008)
  • Author(s): Akifusa, S., et al.
  • Journal Title: Free radical biology & medicine 45 Pages: 1326-1339
  • Peer Reviewed
• [Journal Article] Induction of granulocyte colony-stimulating factor by globular adiponectin via the MEK-ERK pathway (2008)
  • Author(s): Kamio, N., et al.
  • Journal Title: Molecular and Cellular Endocrinology 292 Pages: 20-25
  • Peer Reviewed
• [Journal Article] Relationship between chewing ability and high-level functional capacity in an 80-year-old population in Japan (2008)
  • Author(s): Takata, Y., et al.
  • Journal Title: Gerodontology 25 Pages: 147-154
  • Peer Reviewed
• [Journal Article] Oral Health and risk of mortality from pneumonia in the elderly (2008)
  • Author(s): Awano, S., et al.
  • Journal Title: Journal of Dental Eesearch 87 Pages: 334-339
  • Peer Reviewed
• [Presentation] Low-density lipoprotein induces mitochondrial reactive oxygen species generation in murine macrophage, RAW 264 cells. (2010)
  • Author(s): Akifusa, S.Yamashita, Y.
  • Organizer: Society of Free Radical Biology and Medicine
  • Place of Presentation: Caribe Royale Hotel & Conference Center, Orlando, Florida, USA
  • Year and Date: 2010-11-19
• [Presentation] SOCS1は球状アディポネクチンによって誘導するG-CSFの発現を抑制する (2010)
  • Author(s): 藤本暁江、秋房住郎、廣藤卓雄、山下喜久
  • Organizer: 日本歯科基礎医学会
• [Presentation] 球状アディポネクチンが誘導するマクロファージのアポトーシスにおける活性酸素種の関与について (2009)
  • Author(s): 秋房住郎, 他
  • Organizer: 日本口腔衛生学会
  • Place of Presentation: 長良川国際会議場
  • Year and Date: 2009-10-10
• [Presentation] 球状アディポネクチンが誘導するマクロファージのアポトーシスにおける活性酸素種の関与について (2009)
  • Author(s): 秋房住郎、神尾宜昌、嶋崎義浩、山口登、山下喜久
  • Organizer: 日本口腔衛生学会
• [Presentation] アディポネクチンはジアシルグリセロールキナーゼを介してアポトーシスを誘導する (2009)
  • Author(s): 神尾宜昌、秋房住郎、山口登、野中和明、山下喜久
  • Organizer: 日本歯科基礎医学会
• [Presentation] Adiponectin induces the production of granulocyte colony-stimulating factor in macrophage (2008)
  • Author(s): Kamio, N., et al.
  • Organizer: International Academy of Dental Research
  • Place of Presentation: カナダ国トロントMetro Toronto Convention Centre
  • Year and Date: 2008-07-04
• [Presentation] アディポネクチンは活性酸素種を介してマウスマクロファージにアポトーシスを誘導する (2008)
  • Author(s): 秋房住郎、神尾宜昌、山口登、山下喜久
  • Organizer: 日本歯科基礎医学
• [Presentation] アディポネクチンによるLPS誘導シグナル伝達への影響解析 (2008)
  • Author(s): 神尾宜昌、秋房住郎、山口登、野中和明、山下喜久
  • Organizer: 日本歯科基礎医学会