Effects of cadmium in tobacco on the differentiation of periodontal tissues ; Analysis on the destruction mechanism of periodontal tissues

• Project/Area Number: 20592473
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: SATO Tsutomu The Nippon Dental University, 歯科衛生学科, 教授 (60130671)
• Co-Investigator(Kenkyū-buntansha): TANAKA Tomoko 日本歯科大学, 生命歯学部, 講師 (70307958)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: タバコ / カドミウム / 歯周組織 / 細胞分化 / シグナル伝達 / アポトーシス

Research Abstract

To clarify the mechanism of Cd to destroy periodontal tissues、 several kind of cells including the stem cells derived from dental pulp were studied. The sensitivity of these cells、i.e. stem cells from human dental pulp、mouse embryonic stem cells、human gingival keratinocytes and fibroblasts、 to Cd were different among cells by DNA synthesis and MTT assay. Cd at concentration of 10-3mM induced apoptosis in DPS cells. The rate of apoptosis cells were 40-50 %. It was also suggested that Cd triggered the mitochondorial pathway and the cell membrane receptors causing apoptosis in DPS cells.

Research Products

• [Journal Article] Oral malodorous compound triggers mitochondrial-dependent apoptpsis and causes genomic DNA damage in human gingival epithelial cells. (2010)
  • Author(s): Bogdon Calenic、Ken Yaegaki、Takatoshi Murata、Toshio Imai、Izumi Aoyama、Tsutomu Sato
  • Journal Title: J Periodont Res 45 Pages: 31-37
  • Peer Reviewed
• [Journal Article] Oral malodorous compound activates mitochondrial pathway inducing apoptosis in human gingival fibroblasts (2009)
  • Author(s): Maiko Fujimura、B0gdan Calenic、Ken Yaegaki、Takatoshi Murata、Hisataka Ii、Toshio Imai、Tsutomu Sato、Aoyama Izumi
  • Journal Title: Clinical Oral Investigations (Epub)
  • URL: http://www.springerlink.com/content/h64085462u418279/
  • Peer Reviewed
• [Journal Article] Oral malodorous compound triggers mitochondrial-dependent apoptosis and causes genomic DNA damage in human gingival epithelial cells. (2009)
  • Author(s): Bogdan Calenic、Ken Yaegaki、Murata Takatoshi、Toshio Imai、Izumi Aoyama、Tsutomu Sato、Hisataka Ii
  • Journal Title: J Periodont Res. (Epub)
  • URL: http://www3.interscience.wiley.com/journal/122505741/abstract
  • Peer Reviewed
• [Journal Article] Oral malodorous compound inhibits osteoblast proliferation (2009)
  • Author(s): Toshio Imai、Hisataka Ii、Ken Yaegaki、Takatoshi Murata T、Tsutomu Sato、Takeshi Kamoda
  • Journal Title: J.Periodontol 80 Pages: 2028-2034
  • Peer Reviewed
• [Presentation] タバコ中カドミウムによる歯肉細胞障害機構-サイトカインとアポトーシスの誘導- (2009)
  • Author(s): 佐藤勉, 他4名
  • Organizer: 第58回日本口腔衛生学会総会・大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2009-10-10
• [Presentation] タバコ中カドミウムによる歯肉細胞のサイトカインとアポトーシスの誘導 (2009)
  • Author(s): 佐藤勉, 他3名
  • Organizer: 第52回春季日本歯周病学会学術大会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2009-05-15
• [Presentation] タバコ中カドミウムによる歯肉細胞のサイトカインとアポトーシスの誘導 (2009)
  • Author(s): 佐藤勉、イシュキティエフニコライ、カレニックボクダン、八重垣健
  • Organizer: 第52回春季日本歯周病学会学術大会
• [Presentation] タバコ中カドミウムによる歯肉細胞障害機構-サイトカインとアポトーシスの誘導- (2009)
  • Author(s): 佐藤勉、イシュキティエフニコライ、カレニックボクダン、田中とも子、村田貴俊、八重垣健
  • Organizer: 第58回日本口腔衛生学会総会・大会
• [Presentation] Effect of hydrogen sulfide on differentiation of oeoblaste cell. (2009)
  • Author(s): Toshio IMAI、Hisataka Ii、Takeshi KAMODA、Takatoshi MURATA、Tomoko TANAKA、Tsutomu SATO、Ken YAEGAKI
  • Organizer: IADR