Research for exploring of novel target enzymes of organophosphorous compounds

• Project/Area Number: 20599022
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: National Research Institute of Police Science
• Principal Investigator: TSUGE Kouichiro 科学警察研究所, 法科学第三部, 主任研究官 (90356204)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: 神経ガス / サリン / メタミドホス / 質量分析法 / プロテオーム / 中毒 / ショットガンプロテオミクス

Research Abstract

To explore the novel target enzymes for organophosphorous compound, we established the analytical method for specific detection of OP-combined peptide, using the shotgun proteomics procedure. To apply this method for the complicated sample such as serum, we adopted the purification procedure using TiO2, ZrO2 pre treatment column.

Research Products

• [Journal Article] Mass Spectrometric Identification of Chemical Warfare Agent Adducts with Biological Macromolecule for Verification of Their Exposure (2009)
  • Author(s): Kouichiro Tsuge, Yasuo Seto
  • Journal Title: J. Health Sci Volume: 55 Pages: 879-886
  • NAID: 130000127983
• [Journal Article] Mass Spectrometric Identification of Chemical Warfare Agent Adducts with Biological Macromolecule for Verification of Their Exposure. (2009)
  • Author(s): Kouichiro Tsuge, Yasuo Seto
  • Journal Title: J.Health Sci. 55 Pages: 879-886
  • NAID: 130000127983
• [Journal Article] Mass Spectrometric Identification of Chemical Warfare Agent Adducts with Biological Macromolecule for Verification of Their Exposure (2009)
  • Author(s): Kouichiro Tsuge, Yasuo Seto
  • Journal Title: Journal of Health Science 55 Pages: 879-886
  • NAID: 130000127983
  • Peer Reviewed
• [Presentation] 生体内高分子を標的とした化学兵器曝露の証明法の開発(第1報) (2011)
  • Author(s): 柘浩一郎、瀬戸康雄, ほか
  • Organizer: 日本法中毒学会
  • Place of Presentation: 長崎市
  • Year and Date: 2011-06-11
• [Presentation] earch for novel chemical warfare agents exposure biomarkers using LC-MS/M (2011)
  • Author(s): Koichiro Tsuge, Yasuo Seto, et.al.
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics at Denver, 2011
  • Place of Presentation: 米国デンバー
  • Year and Date: 2011-06-08
• [Presentation] Comprehensive On-site Decontamination Technologies for Chemical and Biological Warfare Agents based on Molecular Recognition Adsorption and Photocatalytic Degradation. (2010)
  • Author(s): Y. Seto, K. Tsuge, et. al.
  • Organizer: PACIFICHEM 2010 at Honolulu
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-15
• [Presentation] Development of mass spectrometric method for identifying novel enzymes targeted by anti-cholinesterase organophosphorus compounds using shotgun proteome strategies (2009)
  • Author(s): K.Tsuge, et al.
  • Organizer: The International Association of Forensic Toxicologists(TIAFT)47th meeting 2009, Geneva
  • Place of Presentation: スイス, ジュネーブ
  • Year and Date: 2009-08-24
• [Presentation] Development of mass spectrometric method for identifying novel enzymes targeted by anti-cholinesterase organophosphorus compounds using shotgun proteome strategies (2009)
  • Author(s): Kouichiro Tsuge, Mieko Kanamori-Kataoka, Isaac Ohsawa, Shintaro Kishi, Takeshi Ohmori, Yasuo Seto, Hirofumi Fukushima
  • Organizer: 46th International Congress on Forensic Toxicology(TIAFT)
  • Place of Presentation: Geneva
• [Presentation] Hirofumi Fukushima Development of mass spectrometric method for identifying novel enzymes targeted by anti-cholinesterase organophosphorus compounds using shotgun proteome strategies. (2009)
  • Author(s): Kouichiro Tsuge, Mieko Kanamori-Kataoka, Isaac Ohsawa, Shintaro Kishi, Takeshi Ohmori, Yasuo Seto
  • Organizer: 46th International Congress on Forensic Toxicology (TIAFT) at Geneva. Annales de Tocxicologie Analytique, 21 (Suppl 1), S1-17