Development of new tumor vaccines using antigen presentation by recycling MHC class II molecules

• Project/Area Number: 20599023
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Department of Clinical Research National Hospital Organization, National Sanyo Hospital
• Principal Investigator: YUSUKE Mimura Department of Clinical Research National Hospital Organization, National Sanyo Hospital, 臨床研究部, 生理生化学研究室長 (00219718)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,360,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2008: ¥1,800,000 (Direct Cost: ¥1,800,000)
• Keywords: 抗原認識 / 抗原提示 / MHCクラスII分子 / CD4+T細胞 / 癌ワクチン / Protein folding / MHC class II molecules / CD4+ T cells / MHC classII molecule

Research Abstract

Efficient antigen presentation to CD4+ T cells is essential for activation of anti-tumor immunity. In order to elicit robust T cell responses with smaller doses of antigen, we have designed a new vaccine in which a CD4+ T cell epitope of ovalbumin is inserted in the complementary determining region of an immunoglobulin molecule. This inserted epitope is supposed to be presented to CD4+ T cells after direct binding to a recycling MHC class II molecule on the surface of an antigen presenting cell and processing in the endosome, in contrast to the known processing pathway involving the late endosomes and lysosomes.

Research Products

• [Journal Article] Prognostic potential of FOXP3 expression in non-small cell lung cancer cells combined with tumor-infiltrating regulatory T cells. (2011)
  • Author(s): Tao, H, Mimura, Y, Aoe, K, Kobayashi, S, Yamamoto, H, Matsuda, E, Okabe, K, Matsumoto, T, Sugi, K, Ueoka, H
  • Journal Title: Lung Cancer in press
• [Journal Article] Identification of cancer stem cell markers in human malignant mesothelioma cells (2011)
  • Author(s): Ghani FI, Yamazaki H, Iwata S, Okamoto T, Aoe K, Okabe K, Mimura Y, Fujimoto N, Kishimoto T, Yamada T, Xu CW, Morimoto C
  • Journal Title: Biochem Biophys Res Commun 407 Pages: 735-42
• [Journal Article] Identification of cancer stem cell markers in human malignant mesothelioma cells (2011)
  • Author(s): Ghani, F.I., 他
  • Journal Title: Biochem Biophys Res Commun Volume: 404 Pages: 735-742
  • Peer Reviewed
• [Journal Article] High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immorbilized beads. (2008)
  • Author(s): Kim, Y-G, Shin, D-S, Yang, Y-H, Gil, G-C, Park, C-G, Mimura, Y, et al.
  • Journal Title: Chem.Biol. 15 Pages: 215-23
• [Journal Article] High-throughput screening of glycan-binding proteins using miniature pig kidney N-glycan-immorbilized beads (2008)
  • Author(s): Kim, Y.-G, Mimura, Y. 他
  • Journal Title: Chemistry & Biology 15 Pages: 215-223
  • Peer Reviewed
• [Presentation] 糖鎖の癌性変化とサイトカインの関係 (2010)
  • Author(s): 三村由香、Davide Zocco、三村雄輔, 他
  • Organizer: 第20回日本サイトメトリー学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-26
• [Presentation] 糖鎖の癌性変化と炎症性サイトカインの関係 (2010)
  • Author(s): 三村由香, 他
  • Organizer: 第20回日本サイトメトリー学会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-26
• [Presentation] Impact of α1-3-linked galactose-containing oligosaccharides of mouse/human chimeric IgG on its Fc effector functions (2010)
  • Author(s): 三村由香, et al.
  • Organizer: 第14回国際免疫学会発表
  • Place of Presentation: 神戸
• [Presentation] Impact of α1-3-linked galactose-containing oligosaccharides of a mouse/human chimeric IgG mutant on its effector functions (2010)
  • Author(s): 三村雄輔, 他
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Induction of anti-tumor immunity via recycling MHC class II-guided processing (2008)
  • Author(s): 三村雄輔, 他
  • Organizer: 第38回日本免疫学会総会・学術集会発表
  • Place of Presentation: 京都
• [Presentation] Induction of antitumor immunity via recycling MHC class II-guided processing (2008)
  • Author(s): 三村雄輔
  • Organizer: 第38回日本免疫学会総会
• [Book] John Wiley & Sons (2009)
  • Author(s): Mimura, Y, Jefferis, R, Mimura-Kimura, Y, Abrahams, J, Rudd, PM
  • Publisher: Glycosylation of therapeutic IgGs, In Therapeutic monoclonal antibodies : From Bench to Clinic ((Ed) An, Z.)
• [Book] Therapeutic Monoclonal Antibodies_From Bench to Clinic (2009)
  • Author(s): Yusuke Mimura (分担)
  • Total Pages: 23
  • Publisher: John Wiley & Sons Inc
• [Remarks] ホームページ等
  • URL: http://www.yamaguchi-hosp.jp/english/researchprograms/Researchprograms.html