Elucidation of phytochrome-type photoreception mechanism based on the comprehensive analysis of cyanobacteriochromes

• Project/Area Number: 20608001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 光生命科学
• Research Institution: The University of Tokyo
• Principal Investigator: NARIKAWA Rei The University of Tokyo, 大学院・総合文化研究科, 助教 (30456181)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 光受容体 / フィトクロム / AnPixJ / 結晶化 / 分光解析 / シアノバクテリア / 結晶構造 / シアノバクテリオクロム / 走光性

Research Abstract

In this study, I performed comprehensive analysis of novel photoreceptor superfamily, cyanobacteriochrome that is distinctive relative of the plant red/far-red photoreceptor, phytochrome. Cyanobacteriochromes have diverse spectral properties. Among these, structural and various spectral analyses such as time-resolved and FTIR measurements of AnPixJ were performed. Further, other various cyanobacteriochromes are comprehensively analyzed. As a result, I could provide valuable insights into the photoperception mechanism of linear tetrapyrrole-binding photoreceptors including phytochrome.

Research Products

• [Journal Article] Cellulose accumulation and a cellulose synthase gene are responsible for cell aggregation in the cyanobacterium Thermosynechococcus vulcanus RKN. (2011)
  • Author(s): Kawano Y., Saotome T., Ochiai Y., Katayama M., Narikawa R., Ikeuchi M.
  • Journal Title: Plant Cell Physiol. (accepted.)
  • NAID: 10029711659
  • Peer Reviewed
• [Journal Article] The cyanobacteriochrome, TePixJ, isomerizes its own chromophore by converting phycocyanobilin to phycoviolobilin. (2011)
  • Author(s): Ishizuka T., Kamiya A., Suzuki H., Narikawa R., Noguchi T., Kohchi T., Inomata K., Ikeuchi M.
  • Journal Title: Biochemistry. 50(6) Pages: 953-961
  • Peer Reviewed
• [Journal Article] Novel supercomplex organization of photosystem I in Anabaena and Cyanophora paradoxa. (2011)
  • Author(s): Watanabe M., Kubota H., Wada H., Narikawa R., Ikeuchi M.
  • Journal Title: Plant Cell Physiol. 52(1) Pages: 162-168
  • NAID: 10029708156
  • Peer Reviewed
• [Journal Article] The cyanobacteriochrome, TePixJ, isomerizes its own chromophore by converting phycocyanobilin to phycoviolobilin (2011)
  • Author(s): Takami Ishizuka
  • Journal Title: Biochemistry Volume: 50 Pages: 953-961
  • Peer Reviewed
• [Journal Article] Novel supercomplex organization of photosystem I in Anabaena and Cyanophora paradoxa (2011)
  • Author(s): Mai Watanabe
  • Journal Title: Plant Cell Physiol. Volume: 52 Pages: 162-168
  • Peer Reviewed
• [Journal Article] Cyanobacteriochrome CcaS regulates phycoerythrin accumulation in Nostoc punctiforme, a group II chromatic adapter. (2010)
  • Author(s): Hirose Y., Narikawa R., Katayama M., Ikeuchi M.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 107(19) Pages: 8854-8859
  • Peer Reviewed
• [Journal Article] Genomic structure of an economically important cyanobacterium Arthrospira (Spirulina) platensis NIES-39. (2010)
  • Author(s): Fujisawa T., Narikawa R., Okamoto S., Ehira S., Yoshimura H., Suzuki I., Masuda T., Mochimaru M., Takaichi S., Awai K., Sekine M., Horikawa H., Yashiro I., Omata S., Takarada H., Katano Y., Kosugi H., Tanikawa S., Ohmori K., Sato N., Ikeuchi M., Fujita N., Ohmori M.
  • Journal Title: DNA Res. 17(2) Pages: 85-103
  • NAID: 130006992893
  • Peer Reviewed
• [Journal Article] Genomic structure of an economically important cyanobacterium, Arthrospira(Spirulina)platensis NIES-39 (2010)
  • Author(s): Takatomo Fujisawa
  • Journal Title: DNA Res. Volume: 17 Pages: 85-103
  • Peer Reviewed
• [Journal Article] Cyanobacteriochrome CcaS regulates phycoerythrin accumulation in Nostocpunctiforme, a group II chromatic adapter (2010)
  • Author(s): Yuu Hirose
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. Volume: 107 Pages: 8854-8859
  • Peer Reviewed
• [Journal Article] An Rrf2-type transcriptional regulator is required for expression of psaAB genes in the cyanobacterium Synechocystis sp.PCC 6803. (2009)
  • Author(s): Midorikawa T., Matsumoto K., Narikawa R., Ikeuchi M.
  • Journal Title: Plant Physiol. 151(2) Pages: 882-892
  • Peer Reviewed
• [Journal Article] The Photosystem II Complex is a Monomer or a Dimer? (2009)
  • Author(s): Watanabe M., Iwai M., Narikawa R., Ikeuchi M.
  • Journal Title: Plant Cell Physiol. 50(9) Pages: 1674-1680
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixJ from Anabaena sp.PCC 7120. (2009)
  • Author(s): Narikawa R., Muraki N., Shiba T., Kurisu G., Ikeuchi M.
  • Journal Title: Acta Crystallogr.Sect.F Struct.Biol.Cryst.Commun. 65(2) Pages: 159-162
  • Peer Reviewed
• [Journal Article] Is the photosystem II complex a monomer or a dimer? (2009)
  • Author(s): Mai Watanabe
  • Journal Title: Pant and Cell Physiology 50 Pages: 1674-1680
  • Peer Reviewed
• [Journal Article] An Rrf2-type transcriptional regulator is required for expression of psaAB genes in the cyanobacterium Synechocystis sp.PCC 6803 (2009)
  • Author(s): Takafumi Midorikawa
  • Journal Title: Plant Physiology 151 Pages: 882-892
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray studies of the chromophore-binding domain of cyanobacteriochrome AnPixj from Anabaena sp. PCC 7120 (2009)
  • Author(s): Rei Narikawa
  • Journal Title: Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 Pages: 159-162
  • Peer Reviewed
• [Journal Article] Characterization of the photoactive GAF domain of the CikA homolog (SyCikA, Slr1969) of the cyanobacterium Synechocystis sp.PCC 6803. (2008)
  • Author(s): Narikawa R., Kohchi T., Ikeuchi M.
  • Journal Title: Photochem.Photobiol.Sci. 7(10) Pages: 1253-9
  • Peer Reviewed
• [Journal Article] Novel cyanobacteriochrome CcaS is green/red light receptor and up-regulates the expression of phycobilisome linker protein by green light. (2008)
  • Author(s): Hirose Y., Shimada T., Narikawa R., Katayama M., Ikeuchi M.
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. 105(28) Pages: 9528-9533
  • Peer Reviewed
• [Journal Article] A novel photoactive GAF domain of cyanobacteriochrome AnPixJ that shows reversible green/red photoconversion. (2008)
  • Author(s): Narikawa R., Fukushima Y., Ishizuka T., Itoh S., Ikeuchi M.
  • Journal Title: J.Mol.Biol. 380(5) Pages: 844-855
  • Peer Reviewed
• [Journal Article] Characterization of the photoactive GAF domain of the CikA homolog (SyCikA, S1r1969) of the cyanobacterium Synechocystis sp. PCC 6803 (2008)
  • Author(s): Rei Narikawa
  • Journal Title: Photochemical and Photobiological Sciences 7 Pages: 1253-1259
  • Peer Reviewed
• [Journal Article] Cyanobacteriochrome CcaS is the green light receptor that induces the expression of phycobilisome linker protein (2008)
  • Author(s): Yuu Hirose
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 105 Pages: 9528-9533
  • Peer Reviewed
• [Journal Article] A novel photoactive GAF domain of cyanobacteriochrome AnPixJ that shows reversible green/red photoconversion (2008)
  • Author(s): Rei Narikawa
  • Journal Title: Journal of Molecular Biology 380 Pages: 844-855
  • Peer Reviewed
• [Presentation] 緑/赤色光変換型シアノバクテリオクロムAnPixJのin vitro再構成解析 (2011)
  • Author(s): 成川礼
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2011-03-21
• [Presentation] 緑/赤色光変換型シアノバクテリオクロムAnPixJのin vitro再構成解析 (2011)
  • Author(s): 成川礼
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学川内北キャンパ
  • Year and Date: 2011-03-21
• [Presentation] 真核藻類における光受容体の分布 (2011)
  • Author(s): 成川礼
  • Organizer: 第5回日本ゲノム微生物学会年会
  • Place of Presentation: 東北学院大学土樋キャンパス
  • Year and Date: 2011-03-15
• [Presentation] 有用シアノバクテリアArthrospira(Spirulina)platensisのゲノム解析 (2010)
  • Author(s): 成川礼
  • Organizer: 第1回日本光合成学会大会
  • Place of Presentation: 東京大学駒場キャンパヌ
  • Year and Date: 2010-06-04
• [Presentation] 有用シアノバクテリアArthrospira(Spirulina)platensisのゲノム解析 (2010)
  • Author(s): 成川礼
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2010-03-18
• [Presentation] 有用シアノバクテリアArthrospira(Spirulina)platensisのゲノム解析 (2010)
  • Author(s): 成川礼
  • Organizer: 第4回日本ゲノム微生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2010-03-07
• [Presentation] シアノバクテリアSynechocystis sp.PCC 6803における走光性を制御する新規光受容体の解析 (2009)
  • Author(s): 成川礼
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形大学小白川キャンパス
  • Year and Date: 2009-09-18
• [Presentation] シアノバクテリアSynechocystis sp.PCC 6803における走光性を制御する新規光受容体の解析 (2009)
  • Author(s): 成川礼
  • Organizer: 日本植物学会第73回大会
  • Place of Presentation: 山形
  • Year and Date: 2009-09-18
• [Presentation] Spectral and structural characterization of a novel cyanobacteriochrome-type photoreceptor AnPixJ (2009)
  • Author(s): 成川礼
  • Organizer: 第13回国際原核光合成生物会議(13^<th> ISPP)
  • Place of Presentation: カナダ
  • Year and Date: 2009-08-14
• [Presentation] Spectral and structural characterization of a novel cyanobacteriochrome-type photoreceptor AnPixJ (2009)
  • Author(s): 成川礼
  • Organizer: 第8回国際テトラピロール会議(8th ICTPPO)
  • Place of Presentation: Asilomar Conference Center(アメリカ,カリフォルニア)
  • Year and Date: 2009-07-31
• [Presentation] Spectral and structural characterization of a novel cyanobacteriochrome-type photoreceptor AnPixJ (2009)
  • Author(s): 成川礼
  • Organizer: 第8回国際テトラピロール会議(8^<th> ICTPPO)
  • Place of Presentation: アメリカ
  • Year and Date: 2009-07-31
• [Presentation] 新規光受容体群シアノバクテリオクロムの構造-機能相関 (2009)
  • Author(s): 成川礼
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋大学東山キャンパス
  • Year and Date: 2009-03-21
• [Presentation] 新規光受容体群シアノバクテリオクロムの構造-機能相関 (2009)
  • Author(s): 成川礼
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] 新規光受容体群シアノバクテリオクロムの構造-機能相関 (2009)
  • Author(s): 成川礼
  • Organizer: 第3回日本ゲノム微生物学会年会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-07
• [Presentation] シアノバクテリアの概日リズム入力系レドックスセンサーCikAのホモログSyCikAの光センサーとしての役割 (2008)
  • Author(s): 成川礼
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知大学朝倉キャンパス
  • Year and Date: 2008-09-26
• [Presentation] シアノバクテリアの概日リズム入力系レドックスセンサーCikAのホモログSyCikAの光センサーとしての役割 (2008)
  • Author(s): 成川礼
  • Organizer: 日本植物学会第72回大会
  • Place of Presentation: 高知
  • Year and Date: 2008-09-26
• [Presentation] Novel crystal structure of red-absorbing form of cyanobacteriochrome AnPixJ-GAF2 (2008)
  • Author(s): 成川礼
  • Organizer: 第21回国際結晶学連合会議(IUCr2008)
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2008-08-26
• [Presentation] Novel crystal structure of red-absorbing form of cyanobacteriochrome AnPixJ-GAF2 (2008)
  • Author(s): Rei Narikawa
  • Organizer: 第21回国際結晶学連合会議(IUCr2008)
  • Place of Presentation: 大阪
  • Year and Date: 2008-08-26