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The study on the role of extrinsic proteins in assembly of photosystem 2 complex

Research Project

Project/Area Number 20608002
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 光生命科学
Research InstitutionThe University of Tokyo

Principal Investigator

MIZUSAWA Naoki  The University of Tokyo, 大学院・総合文化研究科, 助教 (80342856)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords植物生理 / シアノバクテリア / 光合成 / 光化学系II / 表在性蛋白質 / 表在性タンパク / 超分子複合体 / 表在性タンパク質
Research Abstract

Mechanism of assembly of photosystem II complex (PSII) has been investigated focusing on the role of extrinsic proteins in the assembly process of PSII. Psb28 is a newly identified extrinsic protein bound to PSII but its function was unknown. In this study, we analyzed properties of PSII in a disruption mutant of psb28 gene of cyanobacterium. It was found that, in the psb28 mutant, PSII assembly intermediates were accumulated and that a repair process of PSII was significantly inhibited. These results suggest that Psb28 plays an important role in the repair process of PSII.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (39 results)

All 2011 2010 2009 2008

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (27 results) Book (1 results)

  • [Journal Article] Synthesis of fatty acids de novo is required for photosynthetic acclimation of Synechocystis sp.PCC 6803 to high temperature.2010

    • Author(s)
      Nanjo Y., Mizusawa N., Wada H., Slabas A.R., Hayashi H., Nishiyama Y.
    • Journal Title

      Biochim.Biophys.Acta 1797

      Pages: 1483-1490

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of photosystem I complex from Synechocystis sp.PCC 6803 by expressing histidine-tagged subunits.2010

    • Author(s)
      Kubota H., Sakurai I., Katayama K., Mizusawa N., Ohashi S., Kobayashi M., Zhang P., Aro E.M., Wada H.
    • Journal Title

      Biochim.Biophys.Acta 1797

      Pages: 98-105

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of fatty acids de novo is required for photosynthetic acclimation of Synechocystis sp. PCC 6803 to high temperature2010

    • Author(s)
      Nanjo Yohei
    • Journal Title

      Biochimica et Biophyica Acta

      Volume: 1797 Pages: 1483-1490

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification and characterization of photosystem I complex from Synechocystis sp. PCC 6803 by expressing histidine-tagged subunits2010

    • Author(s)
      久保田寿子
    • Journal Title

      Biochimica et Biophyica Acta 1797

      Pages: 98-105

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of digalactosyldiacylglycerol in cellular thermotolerance of Synechocystis sp.PCC 6803.2009

    • Author(s)
      Mizusawa N., Sakata S., Sakurai I., Sato N., Wada H.
    • Journal Title

      Arch.Microbiol. 191

      Pages: 595-601

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Lack of digalactosyldiacylglycerol increases the sensitivity of Synechocystis sp.PCC 6803 to high light stress.2009

    • Author(s)
      Mizusawa N., Sakurai I., Sato N., Wada H.
    • Journal Title

      FEBS Lett. 783

      Pages: 718-722

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of digalactosyldiacylglycerol in cellular thermotolerance of Synechocystis sp.PCC 68032009

    • Author(s)
      水澤直樹
    • Journal Title

      Archives of Microbiology 191

      Pages: 595-601

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      和田元
    • Journal Title

      The role of phosphatidylglycerol in photosynthesis. In Lipids in Photosynthesis : Essential and Regulatory Function (Wada, H., Murata, N.eds.)(Springer)

      Pages: 243-263

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Lack of digalactosyldiacylglycerol increases the sensitivity of Synechocystis sp. PCC 6803 to high light stress2009

    • Author(s)
      Mizusawa Naoki
    • Journal Title

      FEBS Letters 783

      Pages: 718-722

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of phosphatidylglycerol in oxygen-evolving complex of photosystem II.2008

    • Author(s)
      Mizusawa N., Sakurai I., Kubota H., Wada H.
    • Journal Title

      In Energy from the Sun(Allen, J.F., Gantt, E., Golbeck, J.H., Osmond, B., eds.)(Springer.)

      Pages: 463-466

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of phosphatidylglycerol in oxygen-evolving complex of photosystem II2008

    • Author(s)
      Mizusawa Naoki
    • Journal Title

      Energy from the Sun (Allen, J. F., Gantt, E., Golbeck, J.H. , Osmond, B., eds.)

      Pages: 463-466

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Roles of anionic phospholipids, phosphatidylglycerol and cardiolipin, in photosynthetic organisms.2011

    • Author(s)
      Wada H., Katayama K., Tanoue R., Mizusawa N.
    • Organizer
      日本-フィンランド交換セミナー2010
    • Place of Presentation
      岡山ロイヤルホテル、岡山
    • Year and Date
      2011-03-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Roles of anionic phospholipids, phosphatidylglycerol and cardiolipin, in photosynthetic organisms2011

    • Author(s)
      Wada Hajime
    • Organizer
      日本-フィンランド交換セミナー2010
    • Place of Presentation
      岡山ロイヤルホテル(岡山県)
    • Year and Date
      2011-03-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高温ストレス下でのSynechocystis sp.PCC 6803におけるPsb28の機能に関する研究2011

    • Author(s)
      水澤直樹、酒田慎也、久保田寿子、桜井勇、和田元
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      東北大学川内キャンパス、仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp.PCC 6803 under high-light and high-temperature stresses.2011

    • Author(s)
      Mizusawa N., Sakata S., Sakurai I., Kubota H., Sato N., Wada H.
    • Organizer
      日本-フィンランド交換セミナー2010
    • Place of Presentation
      岡山ロイヤルホテル、岡山
    • Related Report
      2010 Final Research Report
  • [Presentation] Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp. PCC 6803 under high-light and high-temperature conditions2011

    • Author(s)
      Mizusawa Naoki
    • Organizer
      日本-フィンランド交換セミナー2010
    • Place of Presentation
      岡山ロイヤルホテル(岡山県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高温ストレス下でのSynechocystis sp. PCC 6803におけるPsb28の機能に関する研究2011

    • Author(s)
      水澤直樹
    • Organizer
      日本植物生理学会第52回大会
    • Place of Presentation
      東北大学川内キャンパス(宮城県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp.PCC 6803 under high-temperature stress.2010

    • Author(s)
      Mizusawa N., Sakata S., Sakurai I., Kubota H., Sato N., Wada H.
    • Organizer
      15th International congress on photosynthesis.
    • Place of Presentation
      BeiJing friendship hotel, 北京
    • Related Report
      2010 Final Research Report
  • [Presentation] Synechocystis sp.PCC 6803の光化学系IにおけるDGDGの機能2010

    • Author(s)
      久保田寿子、水澤直樹、和田元
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本大学黒髪北キャンパス、熊本
    • Related Report
      2010 Final Research Report
  • [Presentation] Synechocystis sp.PCC6803の光化学系IにおけるDGDGの機能2010

    • Author(s)
      久保田寿子
    • Organizer
      日本植物生理学会第51回年会
    • Place of Presentation
      熊本
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光化学系I複合体の簡易精製と新規成分の同定2009

    • Author(s)
      和田元、久保田寿子、片山健太、桜井勇、水澤直樹
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      かずさアカデミアホール、木更津
    • Year and Date
      2009-12-05
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系IIの高温適応における脂肪酸合成の役割2009

    • Author(s)
      西山佳孝、南條洋平、水澤直樹、和田元、林秀則
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      かずさアカデミアホール、木更津
    • Year and Date
      2009-12-05
    • Related Report
      2010 Final Research Report
  • [Presentation] 光合成を支える脂質の機能2009

    • Author(s)
      水澤直樹、桜井勇、酒田慎也、久保田寿子、佐藤直樹、和田元
    • Organizer
      ラン藻ゲノム研究交流会
    • Place of Presentation
      東京大学教養学部、東京
    • Year and Date
      2009-07-04
    • Related Report
      2010 Final Research Report
  • [Presentation] 高温ストレス下でのシアノバクテリアの光合成におけるジガラクトシルジアシルグリセロールの役割2009

    • Author(s)
      水澤直樹、酒田慎也、桜井勇、久保田寿子、佐藤直樹、和田元
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      かずさアカデミアホール、木更津
    • Related Report
      2010 Final Research Report
  • [Presentation] シアノバクテリアにおける光化学系II表在性タンパク質の脂質修飾に関する研究2009

    • Author(s)
      久保田寿子、栗原梓、水澤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] ジガラクトシルジアシルグリセロールの欠乏はSynechocystis sp.PCC 6803の熱感受性を増大させる2009

    • Author(s)
      水澤直樹、酒田慎也、桜井勇、佐藤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 光化学系II複合体アセンブリーに関与するPsb27タンパク質の機能解析2009

    • Author(s)
      内海真希、久保田寿子、水澤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] シアノバクテリアにおけるPsb28タンパク質の機能解析2009

    • Author(s)
      酒田慎也、久保田寿子、桜井勇、水澤直樹、和田元
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋大学東山キャンパス、名古屋
    • Related Report
      2010 Final Research Report
  • [Presentation] 光合成を支える脂質の機能2009

    • Author(s)
      水澤直樹
    • Organizer
      ラン藻ゲノム研究交流会
    • Place of Presentation
      東京
    • Related Report
      2009 Annual Research Report
  • [Presentation] 高温ストレス下でのシアノバクテリアの光合成におけるジガラクトシルジアシルグリセロールの役割2009

    • Author(s)
      水澤直樹
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      木更津
    • Related Report
      2009 Annual Research Report
  • [Presentation] 光化学系I複合体の簡易精製と新規成分の同定2009

    • Author(s)
      和田元
    • Organizer
      かずさDNA研究所研究会「ラン藻の分子生物学2009」
    • Place of Presentation
      木更津
    • Related Report
      2009 Annual Research Report
  • [Presentation] シアノバクテリアにおける光化学系II表在性タンパク質の脂質修飾に関する研究2009

    • Author(s)
      久保田寿子
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] ジガラクトシルジアシルグリセロールの欠乏はSynechocystis sp. PCC6803の熱感受性を増大させる2009

    • Author(s)
      水澤直樹
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 光化学系II複合体アセンブリーに関与するPsb27タンパク質の機能解析2009

    • Author(s)
      内海真希
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] シアノバクテリアにおけるPsb28タンパク質の機能解析2009

    • Author(s)
      酒田慎也
    • Organizer
      日本植物生理学会第50回年会
    • Place of Presentation
      名古屋
    • Related Report
      2008 Annual Research Report
  • [Presentation] 光合成におけるジガラクトシルジアシルグリセロールの機能2008

    • Author(s)
      桜井勇
    • Organizer
      第21回植物脂質シンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2008-11-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] Simple purification and characterization of PSI complex from the Synechocystis sp.PCC 6803 strains expressing His-tagged subunit.2008

    • Author(s)
      Kubota H., Sakurai I., Mizusawa N., Zhang P., Aro E.M., Wada H.
    • Organizer
      7th European workshop on molecular biology of cyanobacteria
    • Place of Presentation
      University of South Bohemia, Ceske Budejovice, Czech Republic
    • Related Report
      2010 Final Research Report
  • [Presentation] Simple purification and characterization of PSI complex from the Synechocystis sp. PCC6803 strains expressing His-tagged subunits2008

    • Author(s)
      Kubota Hisako
    • Organizer
      7th European Workshop on Molecular
    • Place of Presentation
      チェスケー・ブジェヨヴィツェ(チェコ)
    • Related Report
      2008 Annual Research Report
  • [Book] Lipids in Photosynthesis : Essential and Regulatory Function(Wada, H., Murata, N. eds.)(The role of phosphatidylglycerol in photosynthesis.)2009

    • Author(s)
      Wada H., Mizusawa N.
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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