| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
MILI which is essential molecule for spermatogenesis, is concerned with gene suppression through small RNAs (piRNAs). We established GS (Germline Stem) cell lines from the MILI deficient testes. We found that piRNA expression was reduced, DNA methylation of the IAP retrotransposon was impaired, and IAP transcripts were accumulated in the MILI deficient GS cells. We introduced MILI expression in the MILI deficient GS cells and rescued the MILI function in the cells.
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