Analysis of germ stem cells and small RNAs
Project/Area Number |
20610002
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Stem cell biology and medical science
|
Research Institution | Osaka University |
Principal Investigator |
MIYAGAWA Satomi Osaka University, 大学院・生命機能研究科, 招へい教員 (90291153)
|
Co-Investigator(Kenkyū-buntansha) |
KIMURA Tohru 大阪大学, 大学院・生命機能研究科, 准教授 (50280962)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | 精子形成 / 小分子RNA / piRNA / 生殖幹細胞 / レトロトランスポゾン / IAP / DNAメチル化 / GS細胞 / 非コードRNA / 幹細胞 |
Research Abstract |
MILI which is essential molecule for spermatogenesis, is concerned with gene suppression through small RNAs (piRNAs). We established GS (Germline Stem) cell lines from the MILI deficient testes. We found that piRNA expression was reduced, DNA methylation of the IAP retrotransposon was impaired, and IAP transcripts were accumulated in the MILI deficient GS cells. We introduced MILI expression in the MILI deficient GS cells and rescued the MILI function in the cells.
|
Report
(4 results)
Research Products
(33 results)
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[Journal Article] MVH in piRNA Processing and Gene Silencing of Retrotransposons2010
Author(s)
Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, Shiromoto Y, Asada N, Toyoda A, Fujiyama Totoki Y, Shibata T, Kimura T, Nakatsuji N, Noce T, Sasaki H, Nakano T.
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Journal Title
Genes Dev. 23
Pages: 887-892
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