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Generation, differentiation and integration of neural stem cells into neural circuits in the adult olfactory system

Research Project

Project/Area Number 20610004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Stem cell biology and medical science
Research InstitutionNara Medical University

Principal Investigator

TSUBOI Akio  Nara Medical University, 医学部, 教授 (20163868)

Co-Investigator(Kenkyū-buntansha) YOSHIHARA Seiichi  奈良県立医科大学, 医学部, 助教 (90360669)
TAKAHASHI Hiroo  奈良県立医科大学, 医学部, 助教 (20390685)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords嗅覚神経系 / 神経幹細胞 / 嗅細胞 / ニューロスフェア / 嗅球介在ニューロン / 成体神経新生 / 樹状突起 / レンチウイルス / 5T4 / 嗅覚系 / in vitro系 / 移植 / GFP / ノックインマウス / 再生医療
Research Abstract

Olfactory sensory neurons are generated from neural stem cells (NSCs) throughout life. Neurosphere assays are standard studying these NSCs in normal tissues. To isolate NSCs) from the mouse olfactory epithelium, we made neurospheres from the olfactory mucosa by a serum-free culture system. Then, we succeeded to differentiate those to olfactory neurons and astrocytes in media, not supplemented with mitogens such as EGF and FGF. Further, olfactory bulb (OB) interneurons are also generated from NSCs throughout the life. Next, by using the lentiviral system, we have analyzed the sensory input-dependent development of OB interneurons. We injected the lentivirrus (CMV promoter-gapEYFP gene) into the SVZ at neonatal stage, followed by naris occlusion.We found that the odorant-induced activity is necessary for the dendrite elongation and dendrite arborization of OB interneurons.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (54 results)

All 2011 2010 2009 2008 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (37 results) Book (2 results) Remarks (4 results)

  • [Journal Article] Two highly homologous mouse odorant receptors encoded by tandemly linked MOR29A and MOR29B genes differently respond to phenyl ethers.2011

    • Author(s)
      Tsuboi A, Imai T, Kato H, Matsumoto H, Igarashi K, Suzuki M, Mori K, Sakano H
    • Journal Title

      European Journal of Neuroscience 33

      Pages: 205-213

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 脳における匂い感覚地図の形成メカニズム2011

    • Author(s)
      高橋弘雄、坪井昭夫
    • Journal Title

      Foods & Food Ingredients Journal of Japan 第216巻第2号

      Pages: 100-106

    • NAID

      40017284090

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two highly homologous mouse odorant receptors encoded by tandemly-linked MOR29A and MOR29B genes differently respond to phenyl ethers.2011

    • Author(s)
      Tsuboi, A., et al.
    • Journal Title

      European Journal of Neuroscience

      Volume: 33 Pages: 205-213

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sequential arrival and graded secretion of Sema3F by olfactory neuron axons specify map topography at the bulb.2010

    • Author(s)
      Takeuchi H, Inokuchi K, Aoki M, Suto F, Tsuboi A, Matsuda I, Suzuki M, Aiba A, Serizawa S, 吉原誠一, Fujisawa H, Sakano H
    • Journal Title

      Cell 141

      Pages: 1056-1067

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Neuropilin-2 is required for the proper targeting of ventral glomeruli in the mouse olfactory bulb.2010

    • Author(s)
      Takahashi H, Yoshihara S, Nishizumi H, Tsuboi A
    • Journal Title

      Molecular and Cellular Neuroscience 44

      Pages: 233-245

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chondroitin sulfate demarcates astrocytic territories in the mammalian cerebral cortex.2010

    • Author(s)
      Horii-Hayashi N, Tatsumi K, Matsusue Y, Okuda H, Okuda A, Hayashi M, Yano H, Tsuboi A, Nishi M, Yoshikawa M, Wanaka A.
    • Journal Title

      Neuroscience Letters 483

      Pages: 67-72

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 脳における匂い感覚地図の形成メカニズム2010

    • Author(s)
      高橋弘雄、吉原誠一、坪井昭夫
    • Journal Title

      AROMA RESEARCH 第11巻第3号

      Pages: 224-229

    • NAID

      40017284090

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal regulation of neural map formation along the dorsal-ventral axis of the mouse olfactory bulb.2010

    • Author(s)
      Takeuchi, H., et al.
    • Journal Title

      Cell

      Volume: 141 Pages: 1056-1067

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuropilin-2 is required for the proper targeting of ventral glomeruli in the mouse olfactory bulb.2010

    • Author(s)
      Takahashi, H., et al.
    • Journal Title

      Molecular and Cellular Neuroscience

      Volume: 44 Pages: 233-245

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuropilin-2 is required for the proper targeting of ventral glomeruli in the mouse olfactory bulb2010

    • Author(s)
      Takahashi, H., et.al.
    • Journal Title

      Molecular and Cellular Neuroscience (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatiotemporal regulation of neural map formation along the dorsal-ventral axis of the mouse olfactory bulb2010

    • Author(s)
      Takeuchi, H., et.al.
    • Journal Title

      Cell (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The dendrite arborization of olfactory bulb interneurons in an odor-evoked activity-dependent process.2011

    • Author(s)
      吉原誠一, 西村信城, 高橋弘雄, 森憲作, 坪井昭夫
    • Organizer
      Keystone Symposia : Adult Neurogenesis
    • Place of Presentation
      Taos, New Mexico, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] The dendrite arborization of olfactory bulb interneurons in an odor-evoked activity-dependent process.2011

    • Author(s)
      Yoshihara, S., et al.
    • Organizer
      Keystone Symposia : Adult Neurogenesis
    • Place of Presentation
      Taos, NM, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] The dendrite arborization of olfactory bulb interneurons in an odor-evoked activity-dependent process.2010

    • Author(s)
      吉原誠一, 西村信城, 高橋弘雄, 森憲作, 坪井昭夫
    • Organizer
      The 8th International Symposium on "Molecular and Neural Mechanisms of Taste and Olfactory Perception" Kyushu University Station-I and II Collaborative Research
    • Place of Presentation
      Fukuoka, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      坪井昭夫, 高橋弘雄, 森憲作, 吉原誠一
    • Organizer
      EMBO/EMBL Symposium : Structure and Function of Neural Circuits, EMBL
    • Place of Presentation
      Heidelberg, Germany
    • Related Report
      2010 Final Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      坪井昭夫, 高橋弘雄, 森憲作, 吉原誠一
    • Organizer
      Janelia Conference : Form and function of the olfactory system, HHMI
    • Place of Presentation
      Ashburn, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      吉原誠一, 高橋弘雄, 森憲作, 坪井昭夫
    • Organizer
      Cold Spring Harbor Asia Conference : 1st Francis Crick Neuroscience Symposium
    • Place of Presentation
      Suzhou Dushu Lake Conference Center, China
    • Related Report
      2010 Final Research Report
  • [Presentation] The dendrite arborization of olfactory bulb interneurons in an odor-evoked activity-dependent process.2010

    • Author(s)
      吉原誠一、西村信城、高橋弘雄、森憲作、坪井昭夫
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2010 Final Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      吉原誠一、西村信城、高橋弘雄、森憲作、坪井昭夫
    • Organizer
      第33回日本神経科学会大会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2010 Final Research Report
  • [Presentation] 神経活動依存的な嗅球介在ニューロンの発達機構の解明2010

    • Author(s)
      吉原誠一、西村信城、高橋弘雄、森憲作、坪井昭夫
    • Organizer
      第57回日本生化学会近畿支部例会
    • Place of Presentation
      奈良先端科学技術大学院大学ミレニアムホール
    • Related Report
      2010 Final Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      Tsuboi, A., et al.
    • Organizer
      EMBO/EMBL Symposium : Structure and Function of Neural Circuits
    • Place of Presentation
      Heidelberg, Germany
    • Related Report
      2010 Annual Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      Tsuboi, A., et al.
    • Organizer
      Janelia Conference : Form and function of the olfactory system
    • Place of Presentation
      Ashburn, VA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      Yoshihara, S., et al.
    • Organizer
      Cold Spring Harbor Asia Conference : 1st Francis Crick Neuroscience Symposium
    • Place of Presentation
      Suzhou, China
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular basis of CO_2 sensing in the mouse olfactory system.2010

    • Author(s)
      高橋弘雄, ら
    • Organizer
      第33回日本分子生物学会年会 第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2010 Annual Research Report
  • [Presentation] The dendrite arborization of olfactory bulb interneurons in an odor-evoked activity-dependent process.2010

    • Author(s)
      吉原誠一, ら
    • Organizer
      第33回日本分子生物学会年会 第83回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular basis of CO_2 sensing in the mouse olfactory system.2010

    • Author(s)
      高橋弘雄, ら
    • Organizer
      第33回日本神経科学会大会
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2010 Annual Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation.2010

    • Author(s)
      吉原誠一, ら
    • Organizer
      第33回日本神経科学会大会
    • Place of Presentation
      神戸コンベンションセンター
    • Related Report
      2010 Annual Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation2010

    • Author(s)
      吉原誠一, et al.
    • Organizer
      Cold Spring Harbor Asia Conference : 1st Francis Crick Neuroscience Symposium
    • Place of Presentation
      Suzhou, China
    • Related Report
      2009 Annual Research Report
  • [Presentation] Molecular mechanism underlying the odor map formation in the mouse olfactory bulb.2009

    • Author(s)
      坪井昭夫, 高橋弘雄, 澤村正典, 吉原誠一
    • Organizer
      Keystone Symposia : Chemical Senses
    • Place of Presentation
      Lake Tahoe, USA
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Developmental analysis of olfactory bulb interneurons upon neural circuit formation with the lentiviral system2009

    • Author(s)
      吉原誠一, 高橋弘雄, 澤村正典, 坪井昭夫
    • Organizer
      Keystone Symposia : Chemical Senses
    • Place of Presentation
      Lake Tahoe, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular mechanisms underlying formation of the odor map in the mouse brain.2009

    • Author(s)
      高橋弘雄, 吉原誠一, 澤村正典, 坪井昭夫
    • Organizer
      Keystone Symposia : Axonal connections
    • Place of Presentation
      Keystone Resort, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon neural circuit formation.2009

    • Author(s)
      Yoshihara S, Takahashi H, Saito M, Yoshihara Y, Tsuboi A
    • Organizer
      第43回日本味と匂学会大会
    • Place of Presentation
      旭川市民会館
    • Related Report
      2010 Final Research Report
  • [Presentation] 嗅球介在神経細胞の神経活動依存的な発達機構の解析2009

    • Author(s)
      吉原誠一、高橋弘雄、坪井昭夫
    • Organizer
      第32回日本神経科学会大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] マウス嗅覚系におけるCO_2センサー細胞の解析2009

    • Author(s)
      高橋弘雄, ら
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス嗅覚系におけるCO_2センサー細胞の解析2009

    • Author(s)
      高橋弘雄, ら
    • Organizer
      第32回日本神経科学会大会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2009 Annual Research Report
  • [Presentation] Activity-dependent development of olfactory bulb interneurons upon the neural circuit formation2009

    • Author(s)
      吉原誠一, et al.
    • Organizer
      第43回日本味と匂学会大会
    • Place of Presentation
      旭川市民会館
    • Related Report
      2009 Annual Research Report
  • [Presentation] Developmental analysis of olfactory bulb interneurons upon neural circuit formation with the lentiviral system2009

    • Author(s)
      Yoshihara, S., et al.
    • Organizer
      Keystone Symposia : Chemical Sense
    • Place of Presentation
      Lake Tahoe, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular mechanisms underlying formation of the odor map in the mouse brain.2009

    • Author(s)
      Takahashi, H., et al.
    • Organizer
      Keystone Symposia : Axonal connections
    • Place of Presentation
      Keystone, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular mechanisms underlying formation of the odor map in the mouse brain.2008

    • Author(s)
      高橋弘雄, 吉原誠一, 坪井昭夫
    • Organizer
      The 15th International Symposium on Olfaction and Taste
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular mechanisms underlying formation of the odor map in the mouse brain. Symposium "Generation, maintenance and plasticity of neural circuits in the olfactory system".2008

    • Author(s)
      坪井昭夫, 高橋弘雄, 吉原誠一
    • Organizer
      Neuroscience 2008
    • Place of Presentation
      Tokyo International Forum
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス匂い地図形成の分子機構の解析2008

    • Author(s)
      高橋弘雄、吉原誠一、澤村正典、坪井昭夫
    • Organizer
      第31回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2010 Final Research Report
  • [Presentation] エレクトロポレーションおよびレンチウイルスベクターを用いたマウス嗅覚神経回路形成機構の解析2008

    • Author(s)
      吉原誠一、高橋弘雄、坪井昭夫
    • Organizer
      第31回日本神経科学会大会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2010 Final Research Report
  • [Presentation] マウス匂い地図形成の分子機構の解析2008

    • Author(s)
      高橋弘雄、吉原誠一、坪井昭夫
    • Organizer
      第31回日本神経科学会大会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2010 Final Research Report
  • [Presentation] Molecular mechanisms underlying formation of the odor map in the mouse brain.2008

    • Author(s)
      Takahashi, H., et al.
    • Organizer
      The 15th International Symposium on Olfaction and Taste (ISOT)
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2008 Annual Research Report
  • [Presentation] Molecular mechanisms underlying formation of the odor map in the mouse brain. In : Symposium“Generation, maintenance and plasticity of neural circuits in the olfactory system".2008

    • Author(s)
      Tsuboi, A., et al.
    • Organizer
      Neuroscience 2008
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス匂い地図形成の分子機構の解明2008

    • Author(s)
      高橋弘雄ら
    • Organizer
      第30回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report
  • [Presentation] レンチウイルスベクターを用いたマウス嗅覚神経回路形成機構の解明2008

    • Author(s)
      吉原誠一ら
    • Organizer
      第31回日本神経科学会大会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Presentation] マウス匂い地図形成の分子機構の解明2008

    • Author(s)
      高橋弘雄ら
    • Organizer
      第31回日本神経科学会大会
    • Place of Presentation
      東京
    • Related Report
      2008 Annual Research Report
  • [Book] Regulation of expression of odorant receptor genes.THE SENSES : A COMPREHENSIVE REFERENCE (editors, Basbaum, A.I. et al.) Vol.4 (Olfaction & Taste)2008

    • Author(s)
      Tsuboi A, Sakano H
    • Publisher
      Academic Press
    • Related Report
      2010 Final Research Report
  • [Book] 'Regulation of expression of odorant receptor genes' In : The Senses : A COMPREHENIVE REFERENCE, Vol. 4(Olfaction & Taste)2008

    • Author(s)
      Akio Tsuboi & Hitoshi Sakano
    • Publisher
      Academic Press
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.naramed-u.ac.jp/~amrc-lab1/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.naramed-u.ac.jp/~amrc-lab1/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.naramed-u.ac.jp/~amrc-lab1/

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.naramed-u.ac.jp/~amrc-labl/

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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