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Molecular mechanism of sensory placode differentiation from ES cells

Research Project

Project/Area Number 20610007
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Stem cell biology and medical science
Research InstitutionUniversity of Tsukuba

Principal Investigator

TAKASAKI Mami  University of Tsukuba, 大学院・人間総合科学研究科, 助教 (80392009)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords胚性幹細胞 / 外胚葉 / 再生医学 / 発生 / 分化 / プラコード / 発生・分化
Research Abstract

Compared to the research progress made in the subject of neural ectoderm differentiation using the ES cell approach, progress made in non-neural ectoderm differentiation has been slight. To achieve selective differentiation of non-neural ectoderm such as cranial placode and epidermis, We have applied information gained from Xenopus research to SFEB culture (Matsuo-Takasaki et al., 2005 ; Watanabe et al., 2005) using mouse ES cells. We found that BMP4 and Dkk1 are critical factors to induce placode differentiation as reported in Xenopus embryo. This suggests that molecular mechanism of placode differentiation is conserved during evolution. We have also found that one of the extra cellular matrix (ECMs), fibronectin supports efficient cell adhesion and induces epidermal cell differentiation from mouse ES cells. These results suggested that BMP signaling and the interaction with ECMs are both necessary for the development of epidermis in mammalian systems.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (12 results)

All 2010 2009 2008 Other

All Journal Article (3 results) (of which Peer Reviewed: 1 results) Presentation (4 results) Remarks (5 results)

  • [Journal Article] iPS細胞とES細胞の特性と可能性2009

    • Author(s)
      松尾-高崎真美、大根田修
    • Journal Title

      腎と透析 66刊

      Pages: 291-296

    • Related Report
      2010 Final Research Report
  • [Journal Article] iPS細胞とES細胞の特性と可能性2009

    • Author(s)
      松尾-高崎真美,大根田修
    • Journal Title

      腎と透析(東京医学社) 66

      Pages: 291-296

    • Related Report
      2008 Annual Research Report
  • [Journal Article] Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals2008

    • Author(s)
      Mototsugu Eiraku, Kiichi Watanabe, Mami Matsuo-Takasaki, Masako Kawada, Shigenobu Yonemura, Michiru Matsumura, Takafumi Wataya, Ayaka Nishiyama, Keiko Muguruma and Yoshiki Sasai
    • Journal Title

      Cell Stem Cell 3

      Pages: 519-532

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effects of hypoxia on neural differentiation of mouse embryonic stem cells2010

    • Author(s)
      高崎(松尾)真美
    • Organizer
      第16回国際分化学会国際会議
    • Place of Presentation
      奈良県新公会堂(奈良県)
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Analysis of molecular mechanism of non-neural ectoderm differentiation using mouse embryonic stem cells2009

    • Author(s)
      高崎(松尾)真美
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-09
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Molecular mechanisms of early ectodermal development in vertebrate2009

    • Author(s)
      高崎(松尾)真美
    • Organizer
      若手フェスティバルin下田
    • Place of Presentation
      下田臨海実験センター(静岡県)
    • Year and Date
      2009-07-03
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 選択的ROCK阻害剤(Y-27632)を用いたヒトES細胞培養法2008

    • Author(s)
      高崎(松尾)真美
    • Organizer
      日本組織培養学会第81回大会
    • Place of Presentation
      文部科学省研究交流センター(茨城県つくば市)
    • Year and Date
      2008-05-19
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://wakate.biol.tsukuba.ac.jp/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.md.tsukuba.ac.jp/younginit/takasaki/ESteam/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/younginit/takasaki/ESteam/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://wakate.biol.tsukuba.ac.jp/index.html

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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