| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Research Abstract |
Compared to the research progress made in the subject of neural ectoderm differentiation using the ES cell approach, progress made in non-neural ectoderm differentiation has been slight. To achieve selective differentiation of non-neural ectoderm such as cranial placode and epidermis, We have applied information gained from Xenopus research to SFEB culture (Matsuo-Takasaki et al., 2005 ; Watanabe et al., 2005) using mouse ES cells. We found that BMP4 and Dkk1 are critical factors to induce placode differentiation as reported in Xenopus embryo. This suggests that molecular mechanism of placode differentiation is conserved during evolution. We have also found that one of the extra cellular matrix (ECMs), fibronectin supports efficient cell adhesion and induces epidermal cell differentiation from mouse ES cells. These results suggested that BMP signaling and the interaction with ECMs are both necessary for the development of epidermis in mammalian systems.
|
