Regulatory mechanism of ES cells by a novel chromatin-related factor and its application to the reprogramming method of somatic cells.

• Project/Area Number: 20610008
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Stem cell biology and medical science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: KURISAKI Akira National Institute of Advanced Industrial Science and Technology, 幹細胞工学研究センター, 研究チーム長 (60346616)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 幹細胞 / クロマチン / プロテオミクス / ES細胞 / iPS細胞 / 分化 / 転写因子 / リン酸化 / TIF1β / 多能性 / ヒストン / 脱分化

Research Abstract

Although hyperdynamic chromatin is a hallmark of pluripotent stem cells, the mechanisms that regulate these conditions of chromatin were poorly understood. In this study, we found that phosphorylated form of TIF1β is a critical molecule for the maintenance of pluripotency of ES cells. Moreover, phosphorylated form of TIF1β is effective to establish better quality of iPS cells.

Research Products

• [Journal Article] クロマチン制御因子を介した幹細胞未分化制御 (2011)
  • Author(s): 栗崎晃、関泰宏
  • Journal Title: 実験医学 29(1) Pages: 15-20
• [Journal Article] クロマチン制御因子を介した幹細胞未分化制御 (2011)
  • Author(s): 栗崎晃
  • Journal Title: 実験医学 Volume: 29 Pages: 15-20
• [Journal Article] In vitro organogenesis using multipotent cells (2010)
  • Author(s): Kurisaki A., Ito Y., Onuma Y., Intoh A., Asashima M.
  • Journal Title: Human Cell 23(1) Pages: 1-14
  • Peer Reviewed
• [Journal Article] TIF1-beta cooperates with Oct3/4 and regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner. (2010)
  • Author(s): Seki Y., Kurisaki A., Watanabe-Susaki K., Nakajima Y., Nakanishi M., Arai Y., Shiota K., Sugino H., Asashima M.
  • Journal Title: Proc.Natl.Acad.Sci.USA 107(24) Pages: 10926-10931
  • Peer Reviewed
• [Journal Article] TIF1beta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner (2010)
  • Author(s): 関泰宏
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 107 Pages: 10926-10931
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 栗崎晃, 浅島誠
  • Journal Title: 幹細胞の標準化へのアプローチ(iPS細胞の産業的応用技術)(CMC出版) Pages: 26-33
• [Journal Article] Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium (2009)
  • Author(s): Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M.
  • Journal Title: FASEB J. 23 Pages: 114-122
  • Peer Reviewed
• [Journal Article] Proteomic analysis of membrane proteins expressed specifically in pluripotent murine embryonic stem cells (2009)
  • Author(s): Intoh A, Kurisaki A, Yamana ka Y, Hirano H, Fukuda H, Sugino H, Asashima M.
  • Journal Title: Proteomics 9 Pages: 126-137
  • Peer Reviewed
• [Journal Article] Separation with zwitterionic hydrophilic interaction liquid chromatography improves protein identification by matrix-assisted laser desorption/ionization-based proteomic analysis (2009)
  • Author(s): Intoh A, Kurisaki A, Fukuda H, Asashima M.
  • Journal Title: Biomed. Chromatogr (In press)
  • Peer Reviewed
• [Journal Article] (2008)
  • Author(s): Asashima M. Kurisaki K. Michiue T.
  • Journal Title: Stem Cells : from Hydra to Man (共著) In vitro control of organogenesis by ActivinA treatment of amphibian and mouse stem cells(Springer) Pages: 113-124
• [Presentation] ES細胞の未分化性を制御する新規クロマチン関連因子 (2011)
  • Author(s): 栗崎晃
  • Organizer: CREST/さきがけ「iPS細胞」領域合同シンポジウム
  • Place of Presentation: 日本未来館ポスター発表
  • Year and Date: 2011-01-14
• [Presentation] ES細胞の未分化性を制御する新規クロマチン関連因子 (2011)
  • Author(s): 栗崎晃
  • Organizer: CREST/さきがけ[iPS細胞]研究領域合同シンポジウム
  • Place of Presentation: 日本未来館
  • Year and Date: 2011-01-14
• [Presentation] A novel chromatin-related factor that regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner (2010)
  • Author(s): Akira Kurisaki
  • Organizer: 第9回日本再生医療学会
  • Place of Presentation: 広島国際会議場フェニックスホール
  • Year and Date: 2010-03-19
• [Presentation] A novel chromatin-related factor that regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner. (2010)
  • Author(s): 栗崎晃
  • Organizer: 第9回日本再生医療学会総会シンポジウム招待講演
  • Place of Presentation: 広島国際会議場
• [Book] Proteomic analysis of mouse ES cells (2011)
  • Author(s): Kurisaki A., Seki Y, Intoh A
  • Publisher: Embryonic Stem Cells : The Hormonal Regulation of Pluripotency and Embryogenesis.(Edited by : Craig Atwood)(Intech.)
• [Patent(Industrial Property Rights)] 未分化細胞の選別方法 (2008)
  • Inventor(s): 栗崎晃, 浅島誠
  • Industrial Property Rights Holder: 産業技術総合研究所
  • Industrial Property Number: 2008-258302
  • Filing Date: 2008-08-05