• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulatory mechanism of ES cells by a novel chromatin-related factor and its application to the reprogramming method of somatic cells.

Research Project

Project/Area Number 20610008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Stem cell biology and medical science
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

KURISAKI Akira  National Institute of Advanced Industrial Science and Technology, 幹細胞工学研究センター, 研究チーム長 (60346616)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords幹細胞 / クロマチン / プロテオミクス / ES細胞 / iPS細胞 / 分化 / 転写因子 / リン酸化 / TIF1β / 多能性 / ヒストン / 脱分化
Research Abstract

Although hyperdynamic chromatin is a hallmark of pluripotent stem cells, the mechanisms that regulate these conditions of chromatin were poorly understood. In this study, we found that phosphorylated form of TIF1β is a critical molecule for the maintenance of pluripotency of ES cells. Moreover, phosphorylated form of TIF1β is effective to establish better quality of iPS cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2009 2008

All Journal Article (10 results) (of which Peer Reviewed: 6 results) Presentation (4 results) Book (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] クロマチン制御因子を介した幹細胞未分化制御2011

    • Author(s)
      栗崎晃、関泰宏
    • Journal Title

      実験医学 29(1)

      Pages: 15-20

    • Related Report
      2010 Final Research Report
  • [Journal Article] クロマチン制御因子を介した幹細胞未分化制御2011

    • Author(s)
      栗崎晃
    • Journal Title

      実験医学

      Volume: 29 Pages: 15-20

    • Related Report
      2010 Annual Research Report
  • [Journal Article] In vitro organogenesis using multipotent cells2010

    • Author(s)
      Kurisaki A., Ito Y., Onuma Y., Intoh A., Asashima M.
    • Journal Title

      Human Cell 23(1)

      Pages: 1-14

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TIF1-beta cooperates with Oct3/4 and regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner.2010

    • Author(s)
      Seki Y., Kurisaki A., Watanabe-Susaki K., Nakajima Y., Nakanishi M., Arai Y., Shiota K., Sugino H., Asashima M.
    • Journal Title

      Proc.Natl.Acad.Sci.USA 107(24)

      Pages: 10926-10931

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TIF1beta regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner2010

    • Author(s)
      関泰宏
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 107 Pages: 10926-10931

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      栗崎晃, 浅島誠
    • Journal Title

      幹細胞の標準化へのアプローチ(iPS細胞の産業的応用技術)(CMC出版)

      Pages: 26-33

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium2009

    • Author(s)
      Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M.
    • Journal Title

      FASEB J. 23

      Pages: 114-122

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic analysis of membrane proteins expressed specifically in pluripotent murine embryonic stem cells2009

    • Author(s)
      Intoh A, Kurisaki A, Yamana ka Y, Hirano H, Fukuda H, Sugino H, Asashima M.
    • Journal Title

      Proteomics 9

      Pages: 126-137

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Separation with zwitterionic hydrophilic interaction liquid chromatography improves protein identification by matrix-assisted laser desorption/ionization-based proteomic analysis2009

    • Author(s)
      Intoh A, Kurisaki A, Fukuda H, Asashima M.
    • Journal Title

      Biomed. Chromatogr (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2008

    • Author(s)
      Asashima M. Kurisaki K. Michiue T.
    • Journal Title

      Stem Cells : from Hydra to Man (共著) In vitro control of organogenesis by ActivinA treatment of amphibian and mouse stem cells(Springer)

      Pages: 113-124

    • Related Report
      2008 Annual Research Report
  • [Presentation] ES細胞の未分化性を制御する新規クロマチン関連因子2011

    • Author(s)
      栗崎晃
    • Organizer
      CREST/さきがけ「iPS細胞」領域合同シンポジウム
    • Place of Presentation
      日本未来館ポスター発表
    • Year and Date
      2011-01-14
    • Related Report
      2010 Final Research Report
  • [Presentation] ES細胞の未分化性を制御する新規クロマチン関連因子2011

    • Author(s)
      栗崎晃
    • Organizer
      CREST/さきがけ[iPS細胞]研究領域合同シンポジウム
    • Place of Presentation
      日本未来館
    • Year and Date
      2011-01-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] A novel chromatin-related factor that regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner2010

    • Author(s)
      Akira Kurisaki
    • Organizer
      第9回日本再生医療学会
    • Place of Presentation
      広島国際会議場フェニックスホール
    • Year and Date
      2010-03-19
    • Related Report
      2009 Annual Research Report
  • [Presentation] A novel chromatin-related factor that regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner.2010

    • Author(s)
      栗崎晃
    • Organizer
      第9回日本再生医療学会総会シンポジウム招待講演
    • Place of Presentation
      広島国際会議場
    • Related Report
      2010 Final Research Report
  • [Book] Proteomic analysis of mouse ES cells2011

    • Author(s)
      Kurisaki A., Seki Y, Intoh A
    • Publisher
      Embryonic Stem Cells : The Hormonal Regulation of Pluripotency and Embryogenesis.(Edited by : Craig Atwood)(Intech.)
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 未分化細胞の選別方法2008

    • Inventor(s)
      栗崎晃, 浅島誠
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Number
      2008-258302
    • Filing Date
      2008-08-05
    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi