Analysis of vacuole formation by small organic molecules

• Project/Area Number: 20611007
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemical biology
• Research Institution: Kyoto University
• Principal Investigator: KAWAZOE Yoshinori Kyoto University, 化学研究所, 助教 (20402927)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: オルガネラ形成 / 液胞 / 小分子有機化合物 / ケミカルバイオロジー / オルガネラ / オートファジー / オートファジー関連遺伝子 / 膜融合 / エンドソーム

Research Abstract

I discovered a small organic molecule that induces a huge vacuoles in mammalian cells. The aim of this study is elucidating a molecular mechanism of vacuolation by means of this compound as a tool. To examine the vacuole origin, I conducted a immuno-fluorescense microscopic study and found that localizations of AAE1 and M6PR, early and late endosome marker protein, respectively, was different from those under normal state. And I found that endosomes are enlarged by fusion each other. Furthermore, the compound was appeared to induce autophagy.

Research Products

• [Journal Article] Small-molecule fluorescent probes for specific RNA targets. (2011)
  • Author(s): Murata, A., Sato, S., Kawazoe, Y., Uesugi, M.
  • Journal Title: Chem.Commun. 47 Pages: 4712-4714
  • Peer Reviewed
• [Journal Article] Deactivation of STAT6 through serine 707 phosphorilation by JNK. (2011)
  • Author(s): Shirakawa, T., Kawazoe, Y., Tsujikawa, T., Jung, D., Sato, S., Uesugi, M.
  • Journal Title: J.Biol.Chem. 286 Pages: 4003-4010
  • Peer Reviewed
• [Journal Article] A novel screen using the Reck tumor suppressor gene promoter detects both conventional and metastasis-suppressing anticancer drugs. (2010)
  • Author(s): Murai, R., Yoshida, Y., Muraguchi, T., Nishimoto, E., Morioka, Y., Kitayama, H., Kondoh, S., Kawazoe, Y., Uesugi, M., Noda, M.
  • Journal Title: Onco Target 1 Pages: 252-264
  • Peer Reviewed
• [Journal Article] A novel screen using the Reck tumor suppressor gene promoter detects both conventional and metastasis-suppressing anticancer drugs. (2010)
  • Author(s): Murai R, Yoshida Y, Muraguchi T, Nishimoto E, Morioka Y, Kitayama H, Kondoh S, Kawazoe Y, Hiraoka M, Uesugi M, Noda M
  • Journal Title: Oncotarget Volume: 1 Pages: 252-264
  • Peer Reviewed
• [Journal Article] Deactivation of STAT6 through serine 707 phosphorylation by JNK (2010)
  • Author(s): Shirakawa T, Kawazoe Y, Tsujikawa T, Jung D, Sato S, Uesugi M.
  • Journal Title: J.Biol.Chem. Volume: 286 Pages: 4003-4010
  • Peer Reviewed
• [Journal Article] A Small Molecule that Blocks Fat Synthesis by Inhibiting the Activation of SREBP. (2009)
  • Author(s): Kamisuki, S., Mao, Q., Abu-Eliheiga, L., Gu, Z., Kugimiya, A., Kwon, Y., Shinohara, T., Kawazoe, Y., Sato, S. Asakura, K., Choo, H., Sakai, J., Wakil, SJ., Uesugi, M.
  • Journal Title: Chem.Biol. 16 Pages: 882-892
  • NAID: 120002117480
  • Peer Reviewed
• [Journal Article] A small molecule that blocks fat synthesis by inhibiting the activation of SREBP. (2009)
  • Author(s): Kamisuki S, Mao Q, Abu-Elheiga L, Gu Z, Kugimiya A, Kwon Y, Shinohara T, Kawazoe Y, Sato S, Asakura K, Choo HY, Sakai J, Wakil SJ, Uesugi M.
  • Journal Title: Chemistry and Biology 16 Pages: 882-892
  • NAID: 120002117480
  • Peer Reviewed