Application for the fluorescent detection of single nucleotide difference using cytosine bulge hairpin primers
| Project/Area Number |
20611010
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Chemical biology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
TAKEI Fumie Osaka University, 産業科学研究所, 助教 (30252711)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAKATANI Kazuhiko 大阪大学, 産業科学研究所, 教授 (70237303)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | PCR / ヘアピンプライマー / 蛍光 / SNPタイピング / DNA / 蛍光分子 / シトシンバルジ / ヘアピン構造 / シトシンバルジ構造 / 一塩基多型 |
|---|
| Research Abstract |
PCR primer having a cytosine-bulge site at the end of 5' was designed, and using fluorescence molecule, we investigated a novel method of SNP typing. This fluorescence molecule did not inhibit on the PCR, and the hairpin unit was extended in the PCR. The fluorescence molecule binding specifically to C-bulge unit could bind to the PCR-hairpin- primer, however, after PCR, it could bind to the hairpin primer because of disappearance of C-bulge unit. The measurement of fluorescence intensity showed the PCR products, which showed this novel method could be used for the SNP typing.
|
Report
(4 results)
Research Products
(23 results)
-
-
-
[Journal Article] Secondary Structure-Inducible Ligand Fluorescence Coupled with PCR2009
Author(s)
Takei, F., Igarashi, M., Hagihara, M., Oka, Y., Soya, Y., Nakatani, K.
-
Journal Title
Angew.Chem.Int.Ed. 48
Pages: 7822-7824
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
