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Development of specific probes for auxin receptors, TIR1 and ABP1, and Study for auxin signaling.

Research Project

Project/Area Number 20611019
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemical biology
Research InstitutionOkayama University of Science

Principal Investigator

HAYASHI Ken-Ichiro  Okayama University of Science, 理学部, 准教授 (30289136)

Co-Investigator(Kenkyū-buntansha) 野崎 浩  岡山理科大学, 理学部, 教授 (60159085)
Co-Investigator(Renkei-kenkyūsha) NOZAKI Hiroshi  岡山理科大学, 理学部, 教授 (60159085)
STEFAN Kepinski  University of Leeds, Center for Plant Sciences, Ph.D.
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsオーキシン / 分子プローブ / TIR1受容体 / 阻害剤 / 植物ホルモン / ABP1 / F-box / オーキシン受容体 / F-boxタンパク
Research Abstract

Auxin palys a crucial role in plant development. Auxin is perceived by two types of receptors, TIR1 nuclear receptor and ABP1 membrane receptor. These two auxin receptors regulate various auxin responses in plant. Small molecule probes specific for TIR1 and ABP1 were developed to investigate the physiological function of TIR1 and ABP1 receptors in auxin signaling. As the results, small molecule probes were designed to show specific effects for each auxin receptor.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (29 results)

All 2011 2010 2009 2008 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (10 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Alkoxy-auxins are selective inhibitors of auxin transport mediated by PIN, ABCB, and AUX1 transporters.2011

    • Author(s)
      E.Tsuda, H.Yang, T.Nishimura, Y.Uehara,T.Sakai, M.Furutani, T.Koshiba, M.Hirose, H.Nozaki, AS.Murphy, K.Hayashi
    • Journal Title

      J Biol Chem. 286

      Pages: 2354-64

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Alkoxy-auxins are selective inhibitors of auxin transport mediated by PIN, ABCB, and AUX1 transporters.2011

    • Author(s)
      E.Tsuda, et al.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Pages: 2354-2364

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABP1 Mediates auxin inhibition of clathrin-dependent Endocytosis in Arabidopsis.2010

    • Author(s)
      S.Robert, J.Kleine-Vehn, E.Barbez, M.Sauer, T.Paciorek, P.Baster,S.Vanneste, J.Zhang, S.Simon, M.Covanova, K.Hayashi, P.Dhonukshe, Z.Yang, S.Y.Bednarek, A.M.Jones, C.Luschnig, F.Aniento, E.Zazimalova, J.Friml
    • Journal Title

      Cell 143

      Pages: 111-121

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endogenous diterpenes derived from ent-kaurene, a common gibberellin precursor, regulate protonema differentiation of the moss Physcomitrella patens.2010

    • Author(s)
      K.Hayashi, K.Horie, Y.Hiwatashi, H.Kawaide, S.Yamaguchi, A.Hanada, T.Nakashima, M.Nakajima, L.N.Mander, H.Yamane, M.Hasebe, H.Nozaki
    • Journal Title

      Plant Physiol. 153

      Pages: 1085-97

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endogenous diterpenes derived from ent-kaurene, a common gibberellin precursor, regulate protonema differentiation of the moss Physcomitrella patens.2010

    • Author(s)
      K.Hayashi, et al.
    • Journal Title

      Plant Physiololgy

      Volume: 153 Pages: 1085-1097

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis.2010

    • Author(s)
      S.Robert, et al
    • Journal Title

      Cell

      Volume: 143 Pages: 111-121

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] オーキシン その代謝・輸送・情報伝達を制御するケミカルツール2010

    • Author(s)
      林謙一郎
    • Journal Title

      化学と生物(日本農芸化学会機関誌)

      Volume: 4 Pages: 485-492

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Toyocamycin specifically inhibits auxin signaling mediated by SCFTIR1 pathway.2009

    • Author(s)
      K.Hayashi, S.Kamio, Y.Oono,L.B.Townsend, H.Nozaki
    • Journal Title

      Phytochemistry 70

      Pages: 190-97

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential Downward Stream of Auxin Synthesized at the Tip Has a Key Role in Gravitropic Curvature via TIR1/AFBs-Mediated Auxin Signaling Pathways.2009

    • Author(s)
      T.Nishimura, H.Nakano, K.Hayashi, C.Niwa, T.Koshiba
    • Journal Title

      Plant Cell Phyiol 50

      Pages: 1874-1885

    • NAID

      10027344105

    • Related Report
      2010 Final Research Report
  • [Journal Article] Manipulation of intracellular auxin in a single cell by light with esterase-resistant caged auxins.2009

    • Author(s)
      N.Kusaka, J.Maisch, P.Nick, K.Hayashi, H.Nozaki
    • Journal Title

      Chembiochem 10

      Pages: 2195-2202

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential Downward Stream of Auxin Synthesized at the Tip Has a Key Role in Gravitropic Curvature via TIR1/AFBs-Mediated Auxin Signaling Pathways2009

    • Author(s)
      Nishimura T, Nakano H, Hayashi K, Niwa C, Koshiba T
    • Journal Title

      Plant Cell Physiolgy 50

      Pages: 1874-1885

    • NAID

      10027344105

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Manipulation of Intracellular Auxin in a Single Cell by Light with Esterase-Resistant Caged Auxins2009

    • Author(s)
      Kusaka N, Maisch J, Nick P, Hayashi K, Nozaki H
    • Journal Title

      ChemBioChem 10

      Pages: 2195-2202

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toyocamycin specifically inhibits auxin signaling mediated by SCF(TIR1) pathway.2009

    • Author(s)
      Hayashi K, Kamio S, Oono Y, Tow nsend LB, Nozaki H.
    • Journal Title

      Phytochemistry 70

      Pages: 190-197

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Small-molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling.2008

    • Author(s)
      K.Hayashi, X.Tan, N.Zheng, T.Hatate, Y.Kimura, S.Kepinski, H.Nozaki
    • Journal Title

      Proc.Natl.Acad.Sci.U S A 105

      Pages: 5632-37

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Small molecule agonists and antagonists of F-box protein-substrate interactions in auxin perception and signaling2008

    • Author(s)
      K. Hayashi, X. Tan, N. Zheng, T. Hatate, Y. Kimura, S. Kepinski, H. Nozaki
    • Journal Title

      Proc Natl Acad Sci U S A 105

      Pages: 5632-5637

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] TIR1/AFBオーキシン受容体特異的拮抗剤の分子設計2010

    • Author(s)
      廣瀬正和,Stefan Kepinski,嶋田幸久,野崎浩,林謙一郎
    • Organizer
      第45回植物化学調節学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-11-01
    • Related Report
      2010 Final Research Report
  • [Presentation] オーキシンのケミカルバ イオロジー:オーキシン プローブの応用-コケからシロイヌナズナまで-2010

    • Author(s)
      林謙一郎
    • Organizer
      第74回日本植物学会大会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] オーキシンのケミカルバイオロジー:オーキシン プローブの応用-コケからシロイヌナズナまで-2010

    • Author(s)
      林謙一郎
    • Organizer
      第74回日本植物学会大会
    • Place of Presentation
      春日井市
    • Related Report
      2010 Annual Research Report
  • [Presentation] TIR1/AFBオーキシン受容体特異的拮抗剤の分子設計2010

    • Author(s)
      廣瀬正和, Stefan Kepinski, 嶋田幸久, 野崎浩, 林謙一郎
    • Organizer
      第45回植物化学調節学会
    • Place of Presentation
      神戸市
    • Related Report
      2010 Annual Research Report
  • [Presentation] ABP1(Auxin binding Protein 1)特異的プローブの設計と合成2009

    • Author(s)
      中尾幸生, 林謙一郎, 野崎浩
    • Organizer
      第44回植物化学調節学会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] ABP1(Auxin binding Protein 1)特異的プローブの設計と合成2009

    • Author(s)
      中尾幸生,林謙一郎,野崎浩
    • Organizer
      第44回植物化学調節学会
    • Place of Presentation
      仙台
    • Year and Date
      2009-10-29
    • Related Report
      2010 Final Research Report
  • [Presentation] オーキシン分子プローブの開発2009

    • Author(s)
      林謙一郎
    • Organizer
      日本農芸化学会中部支部 第155回例会・シンポジウム・植物のケミカルバイオロジー
    • Place of Presentation
      静岡
    • Year and Date
      2009-07-18
    • Related Report
      2010 Final Research Report
  • [Presentation] オーキシン分子プローブの開発2009

    • Author(s)
      林謙一郎
    • Organizer
      日本農芸化学会中部支部 第155回例会シンポジウム 植物のケミカルバイオロジー
    • Place of Presentation
      静岡市産学交流センター(静岡)
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規オーキシン受容体阻害剤の分子設計と合成2008

    • Author(s)
      林謙一郎,幡手達也,Stefan Kepinski,野崎浩
    • Organizer
      第43植物化学調節学会
    • Place of Presentation
      筑波
    • Year and Date
      2008-10-29
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Chemical Biology for Auxin Signaling and Transport2008

    • Author(s)
      林謙一郎
    • Organizer
      AUXIN2004国際会議
    • Place of Presentation
      マラケッシュ・モロッコ
    • Year and Date
      2008-10-05
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Book] 植物のシグナル伝達-分子と応答-(柿本辰男他編)(オーキシン受容体の拮抗阻害剤の開発)2010

    • Author(s)
      林謙一郎(分担)
    • Publisher
      共立出版
    • Related Report
      2010 Final Research Report
  • [Book] 植物のシグナル伝達-分子と応答-(柿本辰男他編)(共立出版)(Pp216-221)2010

    • Author(s)
      林謙一郎(分担)
    • Total Pages
      237
    • Publisher
      オーキシン受容体の拮抗阻害剤の開発
    • Related Report
      2010 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.dbc.ous.ac.jp/labs/KHayashi/Khayashi.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.dbc.ous.ac.jp/labs/KHayashi/Khayashi.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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