Development of the photochemical panning adapted to low affinity ligands and analysis of neural carbohydrates' functions
| Project/Area Number |
20611021
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Kyushu University of Health and Welfare |
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Principal Investigator |
SADAKANE Yutaka Kyushu University of Health and Welfare, 薬学部, 准教授 (60293304)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAHARA Masahiro 九州保健福祉大学, 薬学部, 教授 (40224828)
KONOHA Keiko 九州保健福祉大学, 薬学部, 助手 (00369175)
OHTSUKA Isao 九州保健福祉大学, 薬学部, 講師 (20389589)
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| Co-Investigator(Renkei-kenkyūsha) |
OHTSUKA Isao 九州保健福祉大学, 薬学部, 講師 (20389589)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 光反応 / アミロイド / プロテオーム / 神経科学 / 糖鎖 / 薬学 / 分析化学 / バイオテクノロジー |
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| Research Abstract |
We established the synthetic methods for photo-reactive carbohydrates, in which carbohydrate reducing end was cyclic form, i.e. natural form. Experiments using the new photo-reactive lactose ligand revealed that cyclic form of lactose showed high affinity and high specificity comparing acyclic form of lactose. We also analyzed the interaction between carbohydrates and Alzheimer's beta-amyloid peptides on the lipid membrane by atomic force microscope, and revealed that the interaction of ganglioside and amyloid peptide generated the specific aggregation on lipid layer.
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Report
(4 results)
Research Products
(82 results)
