| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Spliceostatin A (SSA), a methylated derivative of a potent antitumor microbial metabolite FR901464, was found to cause pre-mRNA accumulation and translation in mammalian cells. SSA inhibits splicing and nuclear retention of pre-mRNA binding to the splicing complex. In this study, I performed chemical localizome analysis using fission yeast for identifying proteins whose subcellular localizations were changed upon treatment with SSA. I found that significant number of proteins altered their localization to the nucleolus and that those proteins were involved in ribosomal biogenesis, suggesting that SSA impinges on ribosomal processing.
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