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構造蛋白異常としての表皮水疱症における協調的ヘテロ接合

Research Project

Project/Area Number 20659172
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionHirosaki University

Principal Investigator

澤村 大輔  Hirosaki University, 大学院・医学研究科, 教授 (60196334)

Co-Investigator(Kenkyū-buntansha) 中野 創  弘前大学, 大学院・医学研究科, 准教授 (90281922)
松崎 康司  弘前大学, 医学部・附属病院, 講師 (50322946)
Project Period (FY) 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2008: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywords表皮水庖症 / P180 / ラミニン5γ2鎖 / VII型コラーゲン / 基底膜蛋白 / ノックアウトマウス / 協調的ヘテロ接合 / 構造タンパク
Research Abstract

表皮水庖症に関連する基底膜の構造蛋白は, 互いに密に結合し, 基底膜の安定に寄与している. 代謝経路で連携する酵素群と空間的に連携する基底膜蛋白群の構成は非常に類似する. しかし, 構造蛋白の遺伝子変異によって生ずる遺伝性患に協調的ヘテロ接合の概念持ち込んだ萌芽的研究は内外にはない.
今回の研究の目的は, 同一の代謝経路に連携する酵素の遺伝子におこる協調的ヘテロ接合状態が, 空間的に連携する基底膜蛋白群の遺伝子異常で発症する表皮水庖症に生じているか否かを検討することにあった.
BP180, ラミニン5γ2鎖, VII型コラーゲン, 各遺伝子のノックアウトマウスのヘテロ接合体を交配することにより, それぞれの2つの遺伝子がヘテロの状態(BP180/LAM5, BP180/COL7, LAM5/COL7), になるものを作成した. その組み合わせにより, 180/LAM5, 180/COL7, LAM5/COL7の3種の, ヘテロ接合が重なった状態が存在する. さらに交配を重ねることにより, 3つ全ての遺伝子がヘテロの状態のヘテロ接合蓄積状態のマウスを作成した.
その結果、肉眼的には、ヘテロ状態の組み合わせでは、水庖などが生じる表皮水庖症の状態は作成できないことが明らかになった。しかしながら、組織学的に観察すると、基底膜にわずかに小さな水庖がみられるマウスも観察され、構造蛋白の遺伝子変異によって生ずる協調的ヘテロ接合が存在する可能性も示唆され、研究の継続が必要と感じられた。

Report

(1 results)
  • 2008 Annual Research Report
  • Research Products

    (13 results)

All 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Remarks (1 results)

  • [Journal Article] Novel p.M1T and recurrent p.G301S mutations in cathepsin C in a Japanese patient with Papillon-Lefevre syndrome : implications for understanding the genotype/phenotype relationship2009

    • Author(s)
      Ochiai T
    • Journal Title

      J Dermatol Sci 53

      Pages: 73-75

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vitamin D3 inhibits expression of bullous pemphigoid antigen 1 through post-transcriptional mechanism without new protein synthesis2008

    • Author(s)
      Yamamoto C
    • Journal Title

      J Dermatol Sci 50

      Pages: 155-158

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Skn-1n, a splice variant that is induced by high calcium concentration and specifically expressed in normal human keratinocytes2008

    • Author(s)
      Nakajima K
    • Journal Title

      J Invest Dermatol 128

      Pages: 1336-1339

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Papillon-Lefevre syndrome and malignant melanoma : a high incidence of melanoma development in Japanese palmoplanter keratoderma patients2008

    • Author(s)
      Nakajima K
    • Journal Title

      Dermatology 217

      Pages: 58-62

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcineurin/NFAT-dependent regulation of 230-kDa bullous pemphigoid antigen (BPAG1) gene expression in normal human epiderma 1 keratinocytes2008

    • Author(s)
      Aizu T
    • Journal Title

      J Dermatol Sci 51

      Pages: 45-51

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transformatipn-specific matrix metalloproteinases (MMP)-7 and MMP-13 are expressed by tumour cells in epidermolysis bullosa-associated squamous cell carcinomas2008

    • Author(s)
      Kivisaari AK
    • Journal Title

      Br J Dermatol 51

      Pages: 45-51

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotype-phenotype correlation in non-Hallopeau-Siemens recessive dystrophic epidermolysis bullosa : The splice site mutation c.6216+5G>T in the COL7A1 gene results in aberrant and normal splicings2008

    • Author(s)
      Hamada T
    • Journal Title

      J Dermatol Sci 50

      Pages: 147-150

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical and Genetic analysis of Fabry disease : report of 6 cases including 3 heterozygous females2008

    • Author(s)
      Nagasaki A
    • Journal Title

      J Dermatol Sci 52

      Pages: 61-64

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A de novo missense mutation in the keratin 13 gene in oral white sponge nevus2008

    • Author(s)
      Nishizawa A
    • Journal Title

      Br J Dermatol 159

      Pages: 974-975

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel homozygous missense mutation in the fatty aldehyde dehydrogenase gene causes Sjogren-Larsson syndrome2008

    • Author(s)
      Nakano H
    • Journal Title

      J Dermatol Sci 52

      Pages: 136-138

    • NAID

      10024457607

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cutaneous pemphigus vulgaris with skin features similar to the classic mucocutaneous type : a case report and review of the literature2008

    • Author(s)
      Shinkuma S
    • Journal Title

      Clin Exp Dermatol 33

      Pages: 724-728

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Usefulness of thermography techniques for evaluating the disease activity in Kimura's disease2008

    • Author(s)
      Shinkuma S
    • Journal Title

      Clin Exp Dermatol 33

      Pages: 768-771

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Remarks]

    • URL

      http://www.med.hirosaki-u.ac.jp/~derma/index.html

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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