Molecular mechanisms for neuronal function via vesicular trafficking

• Project/Area Number: 20670003
• Research Category: Grant-in-Aid for Young Scientists (S)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Kyushu University
• Principal Investigator: SHIRANE Michiko 九州大学, 生体防御医学研究所, 准教授 (90398082)
• Project Period (FY): 2008 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥100,100,000 (Direct Cost: ¥77,000,000、Indirect Cost: ¥23,100,000); Fiscal Year 2012: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2011: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2010: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2009: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000); Fiscal Year 2008: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
• Keywords: 神経細胞 / 脳神経疾患 / 細胞内輸送 / プロトルーディン / FKBP38 / 神経疾患 / Protrudin / シナプス / 小胞輸送 / Rab11 / 分子 / 細胞神経科学 / 神経細胞分化 / 神経管形成 / 小胞膜輸送 / タンパク質複合体

Research Abstract

The purpose of our study was to clarify the regulatory mechanism of intracellular transport in nervous system. Particularly, we aimed at the elucidation of the function of the molecular chaperon protein FKBP38 and the trafficking regulatory protein protrudin. As a result, we revealed the part of the causal mechanisms of neurological disorders such as Parkinson’s disease or hereditary spastic paraplegia.

Assessment Rating

Verification Result (Rating)

B

Research Products

• [Journal Article] Selective escape of proteins from mitochondria during mitophagy (2013)
  • Author(s): Saita, et al
  • Journal Title: Nature Communications Volume: 29(web) Issue: 1 Pages: 1410-1423
  • DOI: 10.1038/ncomms2400
  • Peer Reviewed
• [Journal Article] マイトファジーにおける選択的タンパク質脱出の発見と機構解析 (2013)
  • Author(s): 細田將太郎、白根道子、中山敬一
  • Journal Title: 細胞工学 Volume: (in press)
  • URL: http://gakken-mesh.jp/journal/saibo/
• [Journal Article] Selective escape of proteins from mitochondria during mitophagy (2013)
  • Author(s): Saita, S., *Shirane, M., Nakayama K. I.
  • Journal Title: Nature Comiun. Volume: 4 Pages: 1410-1423
  • Peer Reviewed
• [Journal Article] Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation (2011)
  • Author(s): Matsuzaki, F., Shirane, M., Matsumoto, M., & Nakayama, K.I
  • Journal Title: Mol. Biol. Cell Volume: 22 Issue: 23 Pages: 4602-4620
  • DOI: 10.1091/mbc.e11-01-0068
  • Peer Reviewed
• [Journal Article] Promotion of Neurite Extension by Protrudin Requires Its Interaction with Vesicle-associated Membrane Protein-associated Protein. (2009)
  • Author(s): Saita S., Shirane M., Natsume T., Iemura S., Nakayama, K.I
  • Journal Title: J. Biol. Chem Volume: 284 Issue: 20 Pages: 13766-13777
  • DOI: 10.1074/jbc.m807938200
  • Peer Reviewed
• [Journal Article] Promotion of Neurite Extension by Protrudin Requires Its Interaction with Vesicle-associated Membrane Protein-associated Protein. (2009)
  • Author(s): Saita S., Shirane M., Natsume T., Iemura S., Nakayama KI.
  • Journal Title: J.Biol.Chem. 284 Pages: 13766-13777
  • Peer Reviewed
• [Journal Article] Promotion of neurite extension by protrudin requires its interaction with VAMP-associated protein(VAP) (2009)
  • Author(s): Saita, S., Shirane, M., Nakayama, K.I.
  • Journal Title: J.Biol.Chem. 284 Pages: 13766-13777
  • Peer Reviewed
• [Journal Article] Promotion of neurite extension by protrudin requires its interaction with VAMP-associated protein (VAP) (2009)
  • Author(s): Saita, S., Shirane, M., Nakayama, K. I.
  • Journal Title: J. Biol. Chem. in press
  • Peer Reviewed
• [Journal Article] Regulation of apoptosis and neurite extension by FKBP38 is required for neural tube formation in the mouse. (2008)
  • Author(s): Shirane, M., Ogawa, M., Motoyama, J.,Nakayama, K.I.
  • Journal Title: Genes Cells Volume: 13 Issue: 6 Pages: 635-651
  • DOI: 10.1111/j.1365-2443.2008.01194.x
  • Peer Reviewed
• [Journal Article] リサイクリング小胞輸送による神経突起形成の機構 (2008)
  • Author(s): 白根道子
  • Journal Title: 蛋白質核酸酵素 増刊号 メンブレントラフィックの奔流 Volume: 53 Pages: 2202-2206
  • URL: http://www.kyoritsu-pub.co.jp/
• [Journal Article] FKBP38と相互作用するタンパク (2008)
  • Author(s): 白根道子
  • Journal Title: 生体の科学 Volume: 59 Pages: 548-552
  • URL: http://www.igaku-shoin.co.jp/journalPortal.do?journalPortalId=425
• [Journal Article] Regulation of apoptosis and neurite extension by FKBP38 is required for neural tube formation in the mouse. (2008)
  • Author(s): Shirane, M., Ogawa, M., Motoyama, J., Nakayama, K.I.
  • Journal Title: Genes Cells. 13 Pages: 635-651
  • Peer Reviewed
• [Journal Article] リサイクリング小胞輸送による神経突起形成の機構 (2008)
  • Author(s): 白根道子
  • Journal Title: 蛋白質核酸酵素 メンブレントラフィックの奔流 増刊号 53 Pages: 2202-2206
• [Journal Article] FKBP38と相互作用するタンパク (2008)
  • Author(s): 白根道子
  • Journal Title: 生体の科学 59 Pages: 548-552
• [Journal Article] Regulation of apoptosis and neurite extension by FKBP38 is required for neural tube formation in the mouse. (2008)
  • Author(s): Shirane M., Ogawa M., Motoyama J., Nakayama KI.
  • Journal Title: Genes Cells 13 Pages: 635-651
  • Peer Reviewed
• [Presentation] The mechanism of PtdIns5P-dependent vesicular trafficking in neuron (2012)
  • Author(s): Michiko Shirane
  • Organizer: Neuro2012
  • Place of Presentation: Nagoya
  • Year and Date: 2012-09-18
• [Presentation] 小胞輸送による樹状突起スパイン制御の分子機構 (2011)
  • Author(s): 白根道子
  • Organizer: 第84回生化学会シンポジウム「メンブレントラフィック最近のトレンド」
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] 小胞輸送による樹状突起スパイン制御の分子機構 (2011)
  • Author(s): 白根道子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市(招待講演)
  • Year and Date: 2011-09-24
• [Presentation] プロトルーディンによるスフィンゴ脂質を介したRab11依存的シナプス制御 (2010)
  • Author(s): 白根道子
  • Organizer: 包括脳ネットワーク研究会「神経科学と構造生物学の融合」
  • Place of Presentation: 大阪
  • Year and Date: 2010-10-29
• [Presentation] 神経疾患における細胞内輸送の関与 (2010)
  • Author(s): 白根道子
  • Organizer: 日本農芸化学会西日本支部若手シンポジウム「農芸化学の新展開」
  • Place of Presentation: 福岡
  • Year and Date: 2010-10-23
• [Presentation] Protrudin-binding sphigolipid regulates Rab11-dependent synaptic function via interaction with sphingolipid (2010)
  • Author(s): Shirane, M. and Nakayama, I.
  • Organizer: Hot Spring Harbor Symposium
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-08-20
• [Presentation] Protrudin regulates Rabll-dependent synaptic function via interaction with sphingolipid (2010)
  • Author(s): Shirane, M., Nakayama, I.
  • Organizer: Hot Spring Harbor Symposium
  • Place of Presentation: 福岡
  • Year and Date: 2010-08-20
• [Presentation] Protrudin regulates Rab11-dependent synaptic function via interaction with sphingolipid (2010)
  • Author(s): Shirane, M., Nakayama, I.
  • Organizer: Hot Spring Harbor Symposium
  • Place of Presentation: Fukuoka
  • Year and Date: 2010-08-20
• [Presentation] Protrudinの脂質結合を介した神経機能制御への関与 : Protrudin ノックアウトマウスからの知見 (2009)
  • Author(s): 白根道子
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 2009-12-12
• [Presentation] Mice deficient in protrudin display spastic paraplegia : implications for neuron-glia interaction via sphingolipid binding (2009)
  • Author(s): Shirane, M., Nakayama, I.
  • Organizer: Neuro 2009
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-16
• [Presentation] Protrudin 依存的小胞輸送によるシナプス制御と神経障害 (2009)
  • Author(s): 白根道子, 中山敬一
  • Organizer: MBSJ09
  • Place of Presentation: 横浜
  • Year and Date: 2009-08-17
• [Presentation] プロトルーディン変異マウスにおける神経疾患と小胞輸送との関連 (2009)
  • Author(s): 白根道子
  • Organizer: G蛋白質シグナル公開シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-14
• [Presentation] 神経機能制御におけるプロトルーディン依存的小胞輸送の関与 (2009)
  • Author(s): 白根道子
  • Organizer: Gタンパク質特定領域・膜輸送複合体特定領域合同若手WSシンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2009-01-30
• [Presentation] 脂質のトラフィックによる神経機能制御 (2009)
  • Author(s): 白根道子
  • Organizer: メンブレントラフィック・細胞内ロジスティクス合同シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2009-01-29
• [Presentation] Protrudinの脂質結合を介した神経機能制御への関与:Protrudinノックアウトマウスからの知見 (2008)
  • Author(s): 白根道子
  • Organizer: BMB2008シンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] Protrudinノックアウトマウスにおける神経疾患と膜輸送との関連 (2008)
  • Author(s): 白根道子
  • Organizer: 2008年度シナプス研究会「シナプス成熟と可塑性のダイナミクス」
  • Place of Presentation: 岡崎
  • Year and Date: 2008-12-04
• [Presentation] Protrudin interacts with Rab11-GDP and induces neurite formation by directional membrane trafficking (2008)
  • Author(s): 白根道子
  • Organizer: 日本細胞生物学会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-29
• [Presentation] Protrudin Interacts with Rab11-GDP and Induces Neurite Formation by Directional Membrane Trafficking (2008)
  • Author(s): Shirane, M
  • Organizer: Symposium on Molecular Soft Interactions at Biomembrane Interface
  • Place of Presentation: Fukuoka
  • Year and Date: 2008-01-26
• [Presentation] Protrudin Interacts with Rabll-GDP and Induces Neurite Formation by Directional Membrane Trafficking (2008)
  • Author(s): Shirane, M.
  • Organizer: NAIST-GCOE International Symposium
  • Place of Presentation: 奈良
  • Year and Date: 2008-01-16
• [Presentation] Protrudin Interacts with Rab11-GDP and Induces Neurite Formation by Directional Membrane Trafficking (2008)
  • Author(s): Shirane, M.
  • Organizer: NAIST-GCOE International Symposium
  • Place of Presentation: Nara
  • Year and Date: 2008-01-15
• [Book] メンブレントラフィックの奔流(蛋白質核酸酵素Vol. 53 No. 16)リサイクリング小胞輸送による神経突起形成の機構 (2008)
  • Author(s): 白根道子
  • Total Pages: 5
  • Publisher: 共立出版
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