| Budget Amount *help |
¥104,000,000 (Direct Cost: ¥80,000,000、Indirect Cost: ¥24,000,000)
Fiscal Year 2012: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2009: ¥31,200,000 (Direct Cost: ¥24,000,000、Indirect Cost: ¥7,200,000)
Fiscal Year 2008: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
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| Research Abstract |
In this project, at first, I tried to isolate mutated genes related to self-incompatibility (SI) signaling downstream by using combination between self-compatible mutants and QTL analysis. I identified QTL regulating SI downstream on several chromosomes. Secondly, from surveying Arabidopsis lines, I identified inversion on SP11 gene functioning male S determinant. When repaired SP11 on inversion was introduced into Arabidopsis, I succeeded to take SI Arabidopsis line, and published the data on International Journal “Nature". As other topics, I set up gene catalog expressing on papilla cell by using laser microdissection and next generation sequencer. For characterizing pollen-stigma interaction dynamics, I established time-lapse imaging system for observation by microscope.
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