Simultaneous recording of conformational changes and ionic currents of single-molecular ion channels reveals the relationship between membrane potentials and motions of the channels.

• Project/Area Number: 20679002
• Research Category: Grant-in-Aid for Young Scientists (S)
• Allocation Type: Single-year Grants
• Research Field: General physiology
• Research Institution: University of Fukui
• Principal Investigator: SHIMIZU Hirofumi 福井大学, 医学部, 講師 (50324158)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥79,950,000 (Direct Cost: ¥61,500,000、Indirect Cost: ¥18,450,000); Fiscal Year 2011: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2010: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000); Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000); Fiscal Year 2008: ¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000)
• Keywords: 生体膜 / チャネル / トランスポーター / 能動輸送 / 生理学 / 生体分子 / 生物物理学 / 放射線 / 蛋白質 / 1分子計測(SMD) / 1分子計測 / イオンチャネル / 構造変化 / 放射光 / 機能 / 同時計測 / ダイナミクス

Research Abstract

We developed the Diffracted X-ray Tracking method for targeting the simultaneous recordings of conformational changes and ionic currents in a single ion channel molecule. As a result of this research project we established some essential technologies for the recordings: a high-speed data recording, a fluid exchangeable recording chamber, optimized x-ray spectrum, and an image data analyzing program.

Assessment Rating

Verification Result (Rating)

A

Research Products

• [Journal Article] Structural changes of the KcsA potassium channel upon application of the electrode potential studied by surface-enhanced IR absorption spectroscopy (2013)
  • Author(s): A. Yamakata, H. Shimizu, M. Osawa, S. Oiki
  • Journal Title: Chemical Physics Volume: 02 Pages: 35-35
  • URL: http://dx.doi.org/10.1016/j.chemphys.2013
  • Peer Reviewed
• [Journal Article] ATR-FTIR spectroscopy revealing the different vibrational modes of the selectivity filter interacting with K+ and Na+ in the open and collapsed conformations of the KcsA potassium channel (2012)
  • Author(s): Y. Furutani, H. Shimizu, Y. Asai, T. Fukuda, S.Oiki, H. Kandori
  • Journal Title: Journal of Physical Chemistry Letter Volume: 3 Pages: 3806-3810
  • Peer Reviewed
• [Journal Article] Laser-Triggered Single Molecular Gating Motions of the KcsA Potassium Channels Recorded in a Sub-Millisecond Time Resolution (2012)
  • Author(s): H. Shimizu, M. Iwamoto, T. Konno, A. Royant, D. Setten, L. Guerin, M. Wulff, S. Oiki
  • Journal Title: Biophys. J Volume: 102
• [Journal Article] Single Molecular Gating Dynamics for the KcsA Potassium Channel (2011)
  • Author(s): Shigetoshi Oiki, Hirofumi Shimizu,Masayuki Iwamoto and Takashi Konno
  • Journal Title: Advances in Chemical Physics Volume: Volume 146 Pages: 147-194
  • Peer Reviewed
• [Journal Article] Single Molecular Gating Dynamics for the KcsA Potassium Channel (2011)
  • Author(s): Shigetoshi Oiki, Hirofumi Shimizu, Masayuki Iwamoto, Takashi Konno
  • Journal Title: Advances in Chemical Physics Volume: 146 Pages: 147-194
• [Journal Article] A cytotoxic peptide from amarine sponge exhibits ion channel activity through vectorial-insertion into the membrane (2010)
  • Author(s): Iwamoto, M., Shimizu, H., Muramatsu, I., Oiki, S
  • Journal Title: FEBS Lett
  • Peer Reviewed
• [Journal Article] X線1分子計測法に適合するマイクロ流路測定装置の開発 (2010)
  • Author(s): 清水啓史
  • Journal Title: 福井大学重点研究成果集・明日への挑戦 Pages: 30-31
• [Journal Article] A cytotoxic peptide from a marine sponge exhibits ion channel activity through vectorial-insertion into the membrane (2010)
  • Author(s): Iwamoto M, Shimizu H, Muramatsu I, Oiki S.
  • Journal Title: FEBS Letter Volume: 584(18) Pages: 3995-3999
  • Peer Reviewed
• [Journal Article] Global Twisting Motions during Gating in Kcsa Potassium Channels (2009)
  • Author(s): Shimizu, H., Iwamoto, M., Konno, T., Nihei, A., Sasaki, Y. C., Oiki, S
  • Journal Title: Journal of Physiological Sciences Volume: 59 Pages: 256-256
• [Journal Article] Topological mapping of the asymmetric drug binding to the human ether-a-go-go-related gene product (HERG) potassium channel by use of tandem dimers (2008)
  • Author(s): Myokai, T., Ryu, S., Shimizu, H., Oiki, S.
  • Volume: 73(6)
  • Pages: 1643-51
  • Peer Reviewed
• [Journal Article] Kチャネルのイオン透過機構:新しい流動電位測定法により明らかになったイオン-水流束比 (2008)
  • Author(s): 老木成稔,安藤博之,久野みゆき,清水啓史,岩本真幸
  • Journal Title: 生物物理 Volume: 48 Pages: 246-252
  • NAID: 110006839208
• [Journal Article] Topological mapping of the asymmetric drug binding to the human ether-a-go-go-related gene product (HERG) potassium channel by use of tandem dimers (2008)
  • Author(s): Toshihiko Myokai, Sunghi Ryu, Hirofumi Shimizu, Shigetoshi Oiki
  • Journal Title: Molecular Pharmacology 73 Pages: 1645-41
  • Peer Reviewed
• [Journal Article] Kチャネルのイオン透過機構 : 新しい流動電位測定法により明らかになったイオン-水流束比 (2008)
  • Author(s): 老木成稔, 安藤博之, 久野みゆき, 清水啓史, 岩本真幸
  • Journal Title: 生物物理 48 Pages: 246-252
  • NAID: 110006839208
• [Journal Article] Twisting Conformational Changes of Single KcsA Potassium Channel upon Gating using Diffracted X-ray Tracking (2008)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Yuji C. Sasaki, Shigetoshi Oiki
  • Journal Title: Research Frontiers 2007 Pages: 42-43
• [Journal Article] Twisting Conformational Changes of Single KcsA Potassium Channel upon Gating using Diffracted X-ray Tracking (2007)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Yuji C. Sasaki, Shigetoshi Oiki
  • Journal Title: Research Frontiers Pages: 42-43
• [Journal Article] Masayuki Iwamoto and Takashi Konno : Single Molecular Gating Dynamics for the KcsA Potassium Channel.
  • Author(s): Shigetoshi Oiki, Hirofumi Shimizu
  • Journal Title: Advances in Chemical Physics Volume 146
• [Journal Article] Single Molecular Gating Dynamics for the KcsA Potassium Channel.
  • Author(s): Shigetoshi Oiki, Hirofumi Shimizu, Masayuki Iwamoto Taka shi Konno
  • Journal Title: Advances in Chemical Physics Volume: 146(In press)
• [Presentation] イオンチャネル開閉構造変化のX線1分子動画計測 -分子揺らぎと構造変化の相関の解明を目指して- (2013)
  • Author(s): 清水啓史
  • Organizer: 日本物理学会第68回年次大会 シンポジウム「タンパク質の動きを解き明かす一分子計測の最前線」
  • Place of Presentation: 広島
• [Presentation] Development of a method for recording single molecular gating dynamics of KcsA potassium channel in a sub-millisecond time resolution (2013)
  • Author(s): Hirofumi Shimizu
  • Organizer: Nagoya Symposium Frontiers in Structural Physiology
  • Place of Presentation: Nagoya
• [Presentation] KcsA カリウムイオンチャネルの溶液条件変化応答1 分子開閉ダイナミクスの解析 (2012)
  • Author(s): 清水啓史
  • Organizer: 第50回日本生物物理学会年会,シンポジウム「先端顕微計測が照らす生命の輝き」
  • Place of Presentation: 名古屋
• [Presentation] カリウムチャネルの選択透過性とゲーティング (2012)
  • Author(s): 老木成稔,岩本真幸,炭竃享司,清水啓史
  • Organizer: 平成24年度生理学研究所研究会「膜機能分子の機能・構造ゆらぎの時空間スペクトル解析」
• [Presentation] サブミリ秒時間スケールでのKcsA カリウムイオンチャネル開閉構造変化の1 分子動画計測 (2012)
  • Author(s): 清水啓史
  • Organizer: 第12回日本蛋白質科学会年会,シンポジウム「YouTube 時代の構造生物学」
• [Presentation] Laser-Triggered Single Molecular Gating Motions of the KcsA Potassium Channels Recorded in a Sub-Millisecond Time Resolution (2012)
  • Author(s): H. Shimizu, M. Iwamoto, T. Konno, A. Royant, D. Setten, L. Guerin, M. Wulff, S. Oiki
  • Organizer: Biophysical Society 56th Annual Meeting
  • Place of Presentation: San Diego (USA)
• [Presentation] Development of a diffracted X-ray tracking method for measuring gating transitions of the KcsA potassium channels in a sub-millisecond time resolution (2012)
  • Author(s): Shimizu H., Iwamoto M., Konno T., Royant A., Stetten V.D., Guerin L., Wulff M., Oiki S.
  • Organizer: The 89th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Matsumoto, Japan
• [Presentation] Laser-Triggered Single Molecular Gating Motions of the KcsA Potassium Channels Recorded in a Sub-Millisecond Time Resolution (2012)
  • Author(s): Shimizu H., Iwamoto M., Konno T., Royant A., Stetten V.D., Guerin L., Wulff M., Oiki S.
  • Organizer: 56th Annual Meeting of Biophysical Society
  • Place of Presentation: San Diego, USA(oral)
• [Presentation] サブミリ秒時間スケールでのイオンチャネル構造変化の光トリガーX線1分子計測 (2012)
  • Author(s): 清水啓史
  • Organizer: 特定領域「高次系分子科学」第15回ミニ公開シンポジウム
  • Place of Presentation: 大阪大学(招待講演)
• [Presentation] Gating Motions of KcsA Potassium Channel Measured in a Single Mole cule by Diffracted X-ray Tracking Method (2011)
  • Author(s): Hirofumi Shimizu
  • Organizer: Seminaires at Institute of Physics in University of Rennes 1
  • Place of Presentation: Rennes, France
  • Year and Date: 2011-10-14
• [Presentation] X線1分子計測法によるイオンチャネル開閉構造変化の計測 (2011)
  • Author(s): 清水啓史
  • Organizer: 新学術領域研究「過渡的複合体」公開シンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2011-07-21
• [Presentation] X線1分子計測法によるイオンチャネル開閉構造変化の計測 (2011)
  • Author(s): 清水啓史
  • Organizer: 新学術領域研究「過渡的複合体」公開シンポジウム
  • Place of Presentation: 東京大学(招待講演)
  • Year and Date: 2011-07-21
• [Presentation] Recordings of Gating Motions of the KcsA Potassium Channels in a Single Molecule Level' (2011)
  • Author(s): Hirofumi Shimizu
  • Organizer: "New Developments in Time-Resolved Studies with Synchrotron Radiation" Structural Dynamics Workshop of the ESRF Users' Meeting
  • Place of Presentation: Grenoble, France
  • Year and Date: 2011-02-11
• [Presentation] X線1分子計測法によるカリウムイオンチャネルの1分子ダイナミクスの計測 (2011)
  • Author(s): 清水啓史、岩本真幸、今野卓、Antoine Royant, David Von Stetten, Laurent Guerin, Michael Wulff, 老木成稔
  • Organizer: 第49回日本生物物理学会年会「膜蛋白質の構造変化を研究するための新しい実験ツール」シンポジウム
  • Place of Presentation: 兵庫
• [Presentation] Recordings of Gating Motions of the KcsA Potassium Channels in a Single Molecule Level' "New Developments in Time-Resolved Studies with Synchrotron Radiation (2011)
  • Author(s): Hirofumi Shimizu
  • Organizer: Structural Dynamics Workshop of the ESRF Users' Meeting
  • Place of Presentation: Grenoble, France
• [Presentation] X線1分子計測法によるカリウムイオンチャネルの1分子ダイナミクスの計測 (2011)
  • Author(s): 清水啓史、岩本真幸、今野卓、Antoine Royant, David Von Stetten, Laurent Guerin, Michael Wulff, 老木成稔
  • Organizer: 第49回日本生物物理学会年会「膜蛋白質の構造変化を研究するための新しい実験ツール」シンポジウム
  • Place of Presentation: 兵庫(招待講演)
• [Presentation] Analysis of the Single-molecular Gating Motions of Ion Channels with Difiracted X-ray Tracking Method (2011)
  • Author(s): Hirofumi Shimizu
  • Organizer: 特定領域「高次系分子科学」第5回公開シンポジウム
  • Place of Presentation: 札幌(招待講演)
• [Presentation] イオンチャネル開閉構造変化の光トリガーX線1分子計測システムの開発 (2011)
  • Author(s): 清水啓史, 岩本真幸, 今野卓, Royant A., Stetten V.D., Guerin L., Wulff M., 老木成稔
  • Organizer: 第58回中部生理学会
  • Place of Presentation: 福井
• [Presentation] Recordings of Gating Motions of the KcsA Potassium Channels in a Single Molecule Level (2011)
  • Author(s): Hirofumi Shimizu (Invited)
  • Organizer: "New Developments in Time-Resolved Studies with Synchrotron Radiation"Structural Dynamics Workshop of the ESRF Users' Meeting
  • Place of Presentation: Grenoble, France
• [Presentation] Gating Motions of KcsA Potassium Channel Measured in a Single Molecule by Diffracted X-ray Tracking Method (2010)
  • Author(s): Hirofumi Shimizu
  • Organizer: Institute of Physics University of Rennes
  • Place of Presentation: Rennes, France
  • Year and Date: 2010-12-14
• [Presentation] Gating motions of KcsA potassium channel detected in a single molecule (2010)
  • Author(s): Hirofumi Shimizu
  • Organizer: Institut de Biologie Structurale
  • Place of Presentation: Grenoble, France
  • Year and Date: 2010-09-23
• [Presentation] Gating motions of KcsA potassium channel detected in a single molecule (2010)
  • Author(s): Hirofumi Shimizu
  • Organizer: Seminars in Institut de Biologie Structurale
  • Place of Presentation: Grenoble, France
  • Year and Date: 2010-09-23
• [Presentation] Conformational changes of KcsA potassium channel during gating detected in a single molecule (2010)
  • Author(s): HirofumiShimizu
  • Organizer: Scientific Highlights of Soft Matter Science Day
  • Place of Presentation: Sassenage, France
  • Year and Date: 2010-09-15
• [Presentation] Conformational changes of KcsA potassium channel during gating detect ed in a single molecule (2010)
  • Author(s): Hirofumi Shimizu
  • Organizer: Scientific Highlights of Soft Matter Science Day (European Synchrotron Radiation Facility)
  • Place of Presentation: Sassenage, France
  • Year and Date: 2010-09-15
• [Presentation] Global Twisting Motions of KcsA Potasium Channels upon Gating (2010)
  • Author(s): Hirofumi Shimizu
  • Organizer: PSB Carl-Ivar Branden building
  • Place of Presentation: Grenoble, France
  • Year and Date: 2010-07-07
• [Presentation] Global Twisting Motions of KcsA Potasium Channels upon Gating (2010)
  • Author(s): Hirofumi Shimizu
  • Organizer: Seminar in PSB Carl-Ivar Branden building
  • Place of Presentation: Grenoble, France
  • Year and Date: 2010-07-07
• [Presentation] イオンチャネル開閉構造変化のX線1分子動画計測 (2009)
  • Author(s): 清水啓史
  • Organizer: 第261回触媒化学研究センター談話会
  • Place of Presentation: 北海道大学(招待講演)
  • Year and Date: 2009-08-03
• [Presentation] イオンチャネル開閉構造変化のX線1分子動画計測 (2009)
  • Author(s): 清水啓史
  • Organizer: 第261回触媒化学研究センター談話会
  • Place of Presentation: 北海道大学
  • Year and Date: 2009-08-03
• [Presentation] KcsAカリウムイオンチャネル開閉ねじれ運動のX線1分子計測 (2009)
  • Author(s): 清水啓史, 老木成稔
  • Organizer: 表面・界面を観る非線形分光の新しい展開
  • Place of Presentation: 和光
  • Year and Date: 2009-03-23
• [Presentation] Gating motions ofKcsA potassium channel detected as amovie (2009)
  • Author(s): Hirofumi Shimizu
  • Organizer: Symposium of Molecular Ensemble 2009
  • Place of Presentation: Wako, Saitama
• [Presentation] Conformational Changes of Single-Molecular KcsA Potassium Channels during Gating Recorded in a Sub-Millisecond Time Resolution (2009)
  • Author(s): Hirofumi Shimizu, MasayukiIwamoto, Takashi Konno, Reo Sakakura, Yoshimitsu Aoki, Shigetoshi Oiki
  • Organizer: The 47th annual meeting of the Biophysical Society Japan
  • Place of Presentation: Tokushima
• [Presentation] KcsAカリウムイオンチャネルの構造ダイナミクス研究の現状 (2009)
  • Author(s): 清水啓史、岩本真幸、今野卓、老木成稔
  • Organizer: 第7回ミニ公開シンポジウム「イオンチャネルの構造ダイナミクスII」
  • Place of Presentation: 福井
• [Presentation] KcsAカリウムイオンチャネル開閉ねじれ運動のX線1分子計測 (2009)
  • Author(s): 清水啓史
  • Organizer: 第6回ミニ公開シンポジウム 「表面・界面を観る非線形分光の新しい展開」
  • Place of Presentation: 埼玉 和光 (招待講演)
• [Presentation] Gating motions of KcsA potassium channel detected as a movie (2009)
  • Author(s): Hirofumi Shimizu
  • Organizer: Symposium of Molecular Ensemble 2009
  • Place of Presentation: Wako, Saitama, Invited speaker
• [Presentation] Conformational Changes of Single-Molecular KcsA Potassium Channels during Gating Recorded in a Sub-Millisecond Time Resolution. (2009)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Takashi Konno, Reo Sakakura, Yoshimitsu Aoki , Shigetoshi Oiki
  • Organizer: The 47th annual meeting of the Biophysical Society Japan
  • Place of Presentation: Tokushima
• [Presentation] Gating motions of KcsA potassium channel detected as a movie (2009)
  • Author(s): 清水啓史
  • Organizer: Symposium of Molecular Ensemble 2009
  • Place of Presentation: Wako, Saitama
• [Presentation] KcsAカリウムイオンチャネルの構造ダイナミクス研究の現状 (2009)
  • Author(s): 清水啓史, 岩本真幸, 今野卓, 老木成稔
  • Organizer: 特定領域 高次系分子科学 第7回ミニ公開シンポジウム (オーガナイザー)
  • Place of Presentation: 福井
• [Presentation] Global Twisting Motions During Gating in KcsA Potassium Channels (2009)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Takashi Konno, Amiko Nihei, Yuji C Sasaki
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] KcsAカリウムイオンチャネルの構造ダイナミクス (2008)
  • Author(s): 清水啓史
  • Organizer: 第2回 ミニ公開シンポジウム 「イオンチャネルの構造ダイナミクス」
  • Place of Presentation: 福井
  • Year and Date: 2008-07-09
• [Presentation] イオンチャネル開閉ねじれ運動の1分子動画計測 (2008)
  • Author(s): 清水啓史
  • Organizer: 第26回 ナノ・バイオテクノロジー研究会
  • Place of Presentation: 名古屋工業大学(招待講演)
  • Year and Date: 2008-06-19
• [Presentation] イオンチャネル開閉ねじれ運動の1分子動画計測 (2008)
  • Author(s): 清水啓史
  • Organizer: ナノバイオテクノロジー研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-06-19
• [Presentation] Global Twisting Motionof Single Molecular KcsA PotassiumChannel upon Gating (2008)
  • Author(s): Hirofumi Shimizu, MasayukiIwamoto, Takashi Konno, Amiko Nihei,Shigetoshi Oiki
  • Organizer: New Approaches to Complexity ofProtein Dynamics by Single MoleculeMeasurements : Experiments andTheories
  • Place of Presentation: OsakaGold Poster Award
• [Presentation] Global Twisting Motion of Single Molecular KcsA Potassium Channel Upon Gating. (2008)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Amiko Nihei, Takashi Konno, Yuji C.Sasaki, Shigetoshi Oiki
  • Organizer: New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements : Experiments and Theories
  • Place of Presentation: Osaka Gold Poster Award 受賞
• [Presentation] Visualization of conformational changes of single molecular KcsA potassium channels revealed global twisting motions upon gating. (2008)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Amiko Nihei, Takashi Konno, Yuji C.Sasaki, Shigetoshi Oiki
  • Organizer: The 46th annual meeting of the Biophysical Society Japan
  • Place of Presentation: Fukuoka
• [Presentation] Global Twisting Motion of Single Molecular KcsA Potassium Channel Upon Gating (2008)
  • Author(s): Hirofumi Shimizu, Masayuki Iwamoto, Amiko Nihei, Takashi Konno, Yuji C. Sasaki, Shigetoshi Oiki
  • Organizer: New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements : Experiments and Theories
  • Place of Presentation: 大阪
• [Presentation] Visualization of conformational changes of single molecular Kcs A potassium channels revealed global twisting motions upon gating (2008)
  • Author(s): Hirofumi Shimizu. Masayuki Iwamoto, Amiko Nihei, Takashi Konno, Yuji C. Sasaki, Shigetoshi Oiki
  • Organizer: 日本生物物理学会
  • Place of Presentation: 福岡
• [Presentation] KcsAカリウムチャネルの開閉に伴うねじれ構造変化の1分子動画計測 (2008)
  • Author(s): 清水啓史, 岩本真幸, 今野卓, 二瓶亜三子, 佐々木裕次, 老木成稔
  • Organizer: 日本生化学会
  • Place of Presentation: 神戸
• [Presentation] KcsAカリウムイオンチャネルの構造ダイナミクス (2008)
  • Author(s): 清水啓史
  • Organizer: 分子高次系機能解明のための分子科学 ミニ公開シンポジウム
  • Place of Presentation: 福井
• [Remarks] 2010年文部科学大臣表彰若手科学者賞受賞清水啓史 「イオンチャネル開閉構造変化のX線1分子計測についての研究」
• [Remarks] 清水啓史 「チャネル分子のねじれがイオンの通り道を開閉する」サイエンストピックス60 日本生理学会Web Site 2008/4/13
• [Remarks]
  • URL: http://seiril.labos.ac/ja
• [Remarks] 2010年文部科学大臣表彰若手科学者賞受賞清水啓史
• [Remarks] イオンチャネル開閉構造変化のX線1分子計測についての研究