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Fabrication of the artificial tissue by using porous sheets and laser tweezers

Research Project

Project/Area Number 20680024
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biomedical engineering/Biological material science
Research InstitutionHokkaido University

Principal Investigator

SUNAMI Hiroshi  Hokkaido University, 大学院・先端生命科学研究院, 研究員 (50374723)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
Keywords人工臓器工学 / 再生医工学 / 多孔性シート / 細胞マニュピレーション / 共培養 / 細胞集団 / シグナル伝達 / 低分子量Gタンパク質 / カドヘリン
Research Abstract

In order to design cell clusters as large as a small tissue, I investigated the cellular signaling and the lipid droplet accumulation depending on the formation of cell clusters. Cells were plated on a pattern substrate so that cell clusters with 2-8 cells could be formed, respectively. It was observed that small G-proteins activated strongly in the joined part between cells. Moreover, it was observed that the amount of accumulated lipid droplets increased with an increase in the number of joined cell in a single cell cluster.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (6 results)

All 2011 2010 2009

All Presentation (6 results)

  • [Presentation] 三次元パターンを利用した細胞分離2011

    • Author(s)
      角南寛, 光武進, 五十嵐靖之
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル(東京)
    • Year and Date
      2011-03-02
    • Related Report
      2010 Final Research Report
  • [Presentation] 三次元パターンを利用した細胞分離2011

    • Author(s)
      角南寛, 光武進, 五十嵐靖之
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2011-03-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 三次元パターンを利用した細胞遊走の制御2010

    • Author(s)
      角南寛, 光武進, 五十嵐靖之
    • Organizer
      第33回分子生物学会年会・第83回日本生化学大会合同大会(BMB2010)
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2010-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 三次元パターンを利用した細胞遊走の制御2010

    • Author(s)
      角南寛, 光武進, 五十嵐靖之
    • Organizer
      第33回分子生物学会年会・第83回日本生化学大会合同大会(BMB2010)
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞内スフィンゴミエリン量の細胞増殖及び糖脂質合成に与える影響2009

    • Author(s)
      座間宏太, 林遥, 光武進, 角南寛, 山下匡, 渡辺研, 岡崎俊郎, 五十嵐靖之
    • Organizer
      第82回日本生化学会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2009-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 細胞内スフィンゴミエリン量の細胞増殖及び糖脂質合成に与える影響2009

    • Author(s)
      座間宏太, 林遥, 光武進, 角南寛, 山下匡, 渡辺研, 岡崎俊郎, 五十嵐靖之
    • Organizer
      第82回日本生化学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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