Fabrication of the artificial tissue by using porous sheets and laser tweezers
| Project/Area Number |
20680024
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SUNAMI Hiroshi Hokkaido University, 大学院・先端生命科学研究院, 研究員 (50374723)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥14,820,000 (Direct Cost: ¥11,400,000、Indirect Cost: ¥3,420,000)
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| Keywords | 人工臓器工学 / 再生医工学 / 多孔性シート / 細胞マニュピレーション / 共培養 / 細胞集団 / シグナル伝達 / 低分子量Gタンパク質 / カドヘリン |
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| Research Abstract |
In order to design cell clusters as large as a small tissue, I investigated the cellular signaling and the lipid droplet accumulation depending on the formation of cell clusters. Cells were plated on a pattern substrate so that cell clusters with 2-8 cells could be formed, respectively. It was observed that small G-proteins activated strongly in the joined part between cells. Moreover, it was observed that the amount of accumulated lipid droplets increased with an increase in the number of joined cell in a single cell cluster.
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Report
(4 results)
Research Products
(6 results)
