|Budget Amount *help
¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2008: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
The purpose of this study is to elucidate how forces applied to cells are transmitted through intracellular components. We found that cytoskeletal filaments, such as actin stress fibers(SFs) and microtubules, have crucial roles in maintaining whole cell mechanical properties, and they contribute to the intracellular force balance three dimensionally, which control the internal tension of cells and their three dimensional morphology. We also found that SFs firmly connected to nuclear membrane, and the internal force of SFs transmitted mechanically to the nuclear membrane. These direct force transmission may induce the conformation changes of intranuclear DNA. The results of this study will provide new prospects in cellular mechanotransduction.