Mitochondrial genome/protein toxicity mechanism of aldehyde.

• Project/Area Number: 20681006
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: ISSE Toyohi 産業医科大学, 医学部, 准教授 (80341494)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: トキシコロジー / アセトアルデヒド / 発がん性 / 健康影響 / アルデヒド / ミトコンドリア / ゲノム付加体 / 蛋白付加体 / 毒性 / ミトコンドリアゲノム / 蛋白質付加体

Research Abstract

Levels of acetaldehyde-derived DNA adducts were significantly increased in ethanol and acetaldehyde exposed Aldh2 knockout mouse organs which were model for inactive aldehyde dehydrogenase 2 polymorphism Japanese population than wild type mouse. Our finding supports epidemiological findings on the carcinogenicity of alcohol and acetaldehyde toxicity in ALDH2-deficient individuals who drink chronically and/or who expose by in room air pollution from the biological mechanism aspects.

Research Products

• [Journal Article] Effects and Action Mechanisms of Korean Pear(Pyrus pyrifolia cv. Shingo) on Alcohol Detoxification (2012)
  • Author(s): Lee HS, Isse T, Kawamoto T, Woo HS, Kim AK, Park JY, Yang M.
  • Journal Title: Phytother Res Volume: 17 Issue: 11 Pages: 269-274
  • DOI: 10.1002/ptr.4630
  • Peer Reviewed
• [Journal Article] Aldehyde Dehydrogenase-2 Deficiency Aggravates Cardiac Dysfunction Elicited by Endoplasmic Reticulum Stress Induction (2012)
  • Author(s): Liao J, Sun A, Xie Y, Isse T, Kawamoto T, Zou Y, Ge J.
  • Journal Title: Mol Med Volume: (in press) Issue: 5 Pages: 785-793
  • DOI: 10.2119/molmed.2011.00466
  • Peer Reviewed
• [Journal Article] The effect of ethanol on the formation of N((2))-ethylidene-dG adducts in mice : implications for alcohol-related carcinogenicity of the oral cavity and esophagus. (2012)
  • Author(s): Yu HS, Oyama T, Matsuda T, Isse T, Yamaguchi T, Tanaka M, Tsuji M, Kawamoto T.
  • Journal Title: Biomarkers Volume: 17巻 Pages: 269-274
  • URL: http://informahealthcare.com/doi/abs/10.3109/1354750X.2012.666675
  • Peer Reviewed
• [Journal Article] The effect of ethanol on the formation of N ((2))-ethylidene-dG adducts in mice : implications for alcohol-related carcinogenicity of the oral cavity and esophagus (2012)
  • Author(s): Yu HS
  • Journal Title: Biomaker Volume: (in press)
  • Peer Reviewed
• [Journal Article] Reduced bone formation in alcohol-induced osteopenia is associated with elevated p21 expression in bone marrow cells in aldehyde dehydrogenase 2-disrupted mice (2011)
  • Author(s): Shimizu Y, Sakai A, Menuki K, Mori T, Isse T, Oyama T, Kawamoto T, Nakamura T.
  • Journal Title: Bone Volume: 48巻 Pages: 1075-1086
  • URL: http://www.sciencedirect.com/science/article/pii/S8756328211000123
  • Peer Reviewed
• [Journal Article] Reduced bone formation in alcohol-induced osteopenia is associated with elevated p21 expression in bone marrow cells in aldehyde dehydrogenase 2-disrupted mice (2011)
  • Author(s): Shimizu Y
  • Journal Title: Bone Volume: 48 Pages: 1075-1086
  • Peer Reviewed
• [Journal Article] Formation of acetaldehyde-derived DNA adducts due to alcohol exposure. (2010)
  • Author(s): Yu HS, Oyama T, Isse T, Kitagawa K, Pham TT, Tanaka M, Kawamoto T.
  • Journal Title: Chem Biol Interact Volume: 188巻 Pages: 367-375
  • URL: http://www.sciencedirect.com/science/article/pii/S0009279710005259
• [Journal Article] Effects of acetaldehyde inhalation in mitochondrial aldehyde dehydrogenase deficient mice(Aldh2-/-) (2010)
  • Author(s): Oyama T, Nagayoshi H, Matsuda T, Oka M, Isse T, Yu HS, Pham TT, Tanaka M, Kagawa N, Kaneko K, Kawamoto T.
  • Journal Title: Front Biosci(Elite Ed) Volume: 1巻 Pages: 1344-1354
  • URL: http://www.bioscience.org/2010/V2E/af/194/fulltext.htm
  • Peer Reviewed
• [Journal Article] Formation of acetaldehyde-derived DNA adducts due to alcohol exposure. (2010)
  • Author(s): Yu HS, Oyama T, Isse T, Kitagawa K, Pham TT, Tanaka M, Kawamoto T.
  • Journal Title: Chem Biol Interact. 188巻 Pages: 367-375
  • Peer Reviewed
• [Journal Article] Effects of acetaldehyde inhalation in mitochondrial aldehyde dehydrogenase deficient mice(Aldh2-/-). (2010)
  • Author(s): Oyama T, Nagayoshi H, Matsuda T, Oka M, Isse T, Yu HS, Pham TT, Tanaka M, Kagawa N, Kaneko K, Kawamoto T.
  • Journal Title: Front Biosci. 2巻 Pages: 1344-1354
  • Peer Reviewed
• [Journal Article] Formation of acetaldehyde-derived DNA adducts due to alcohol exposure. (2010)
  • Author(s): Yu HS, Oyama T, Isse T, et al
  • Journal Title: Chem Biol Interact. Volume: 188 Pages: 367-375
  • Peer Reviewed
• [Journal Article] Effects of acetaldehyde inhalation in mitochondrial aldehyde dehydrogenase deficient mice (Aldh2-/-). (2010)
  • Author(s): Oyama T, Nagayoshi H, et al.
  • Journal Title: Front Biosci. Volume: 2 Pages: 1344-1354
  • Peer Reviewed
• [Journal Article] Characteristics of aldehyde dehydrogenase 2(Aldh2) knockout mice (2009)
  • Author(s): Yu HS, Oyama T, Isse T, Kitakawa K, Ogawa M, Pham TT, Kawamoto T.
  • Journal Title: Toxicol Mech Methods Volume: 19巻 Pages: 535-540
  • URL: http://informahealthcare.com/doi/abs/10.3109/15376510903401708
  • Peer Reviewed
• [Journal Article] Cholinergic alterations following alcohol exposure in the frontal cortex of Aldh2-deficient mice models (2009)
  • Author(s): Jamal M, Ameno K, Miki T, Wang W, Kumihashi M, Isse T, Kawamoto T, Kitagawa K, Nakayama K, Ijiri I, Kinoshita H.
  • Journal Title: Brain Res Volume: 1295巻 Pages: 37-43
  • URL: http://www.sciencedirect.com/science/article/pii/S0006899309015935
  • Peer Reviewed
• [Journal Article] Application of tryptophan fluorescence to assess sensitizing potentials of chemicals (2009)
  • Author(s): Pham TT, Oyama T, Isse T, Kawamoto T
  • Journal Title: Arch Environ Contam Toxicol Volume: 57巻 Pages: 427-436
  • URL: http://www.springerlink.com/content/u8qh1q2366445482/?MUD=MPEpub
  • Peer Reviewed
• [Journal Article] Characteristics of aldehyde dehydrogenase 2(Aldh2)knockout mice. (2009)
  • Author(s): Yu HS, Oyama T, Isse T, Kitakawa K, Ogawa M, Pham TT, Kawamoto T.
  • Journal Title: Toxicol Mech Methods. 19巻 Pages: 535-540
  • Peer Reviewed
• [Journal Article] Cholinergic alterations following alcohol exposure in the frontal cortex of Aldh2-deficient mice models. (2009)
  • Author(s): Jamal M, Ameno K, Miki T, Wang W, Kumihashi M, Isse T, Kawamoto T, Kitagawa K, Nakayama K, Ijiri I, Kinoshita H.
  • Journal Title: Brain Res. 1295巻 Pages: 37-43
  • Peer Reviewed
• [Journal Article] Application of tryptophan fluorescence to assess sensitizing potentials of chemicals. (2009)
  • Author(s): Pham TT, Oyama T, Isse T, Kawamoto T.
  • Journal Title: Arch Environ Contam Toxicol 1295巻 Pages: 427-436
  • Peer Reviewed
• [Journal Article] Characteristics of aldehyde dehydrogenase 2(A1dh2) knockout mice (2009)
  • Author(s): Yu HS, Oyama T, Isse T, Kitakawa K, Ogawa M, Pham TT, Kawamoto T.
  • Journal Title: Toxicol Mech Methods. 19 Pages: 535-540
  • Peer Reviewed
• [Journal Article] Cholinergic alterations following alcohol exposure in the frontal cortex of A1dh2-deficient mice models (2009)
  • Author(s): Jamal M, Ameno K, Miki T, Wang W, Kumihashi M, Isse T, Kawamoto T, Kitagawa K, Nakayama K, Ijiri I, Kinoshita H.
  • Journal Title: Brain Res. 1295 Pages: 37-43
  • Peer Reviewed
• [Journal Article] Application of tryptophan fluorescence to assess sensitizing potentials of chemicals (2009)
  • Author(s): Pham TT, Oyama T, Isse T, Kawamoto T.
  • Journal Title: Arch Environ Contam Toxicol 1295 Pages: 427-436
  • Peer Reviewed
• [Journal Article] Effects of 5-week ethanol feeding on the liver of aldehyde dehydrogenase 2 knockout mice (2008)
  • Author(s): Matsumoto A, Kawamoto T, Mutoh F, Isse T, Oyama T, Kitagawa K, Nakayama KI, Ichiba M
  • Journal Title: Pharmacogenet Genomics Volume: 18巻 Pages: 847-852
  • Peer Reviewed
• [Journal Article] Effects of 5-week ethanol feeding on the liver of aldehyde dehydrogenase 2 knockout mice. (2008)
  • Author(s): Matsumoto A, Kawamoto T, Mutoh F, Isse T, Oyama T, Kitagawa K, Nakayama KI, Ichiba M.
  • Journal Title: Pharmacogenet Genomics. 18巻 Pages: 47-52
  • Peer Reviewed
• [Journal Article] Increased frequencies of micronucleated reticulocytes and T-cell receptor mutation in Aldh2 knockout mice exposed to acetaldehyde. (2008)
  • Author(s): Kunugita N, Isse T, Oyama T, Kitagawa K, Ogawa M, Yamaguchi T, Kinaga T, Kawamoto T.
  • Journal Title: J Toxicol Sci. 33巻 Pages: 31-36
  • NAID: 110006596029
  • Peer Reviewed
• [Journal Article] Effects of 5-week ethanol feeding on the liver of aldehyde dehydrogenase 2 knockout mice (2008)
  • Author(s): Matsumoto A, Kawamoto T, Mutoh F, Isse T, Oyama T, Kitagawa K, Nakayama KI, Ichiba M.
  • Journal Title: Pharmacogenet Genomics 18 Pages: 47-52
  • Peer Reviewed
• [Journal Article] Increased frequencies of micronucleated reticulocytes and T-cell receptor mutation in Aldh2 knockout mice exposed to acetaldehyde (2008)
  • Author(s): Kunugita N, Isse T, Oyama T, Kitagawa K, Ogawa M, Yamaguchi T, Kinaga T, Kawamoto T.
  • Journal Title: J Toxicol Sci 33 Pages: 31-36
  • NAID: 110006596029
  • Peer Reviewed
• [Presentation] The survivals and body weight variations of aldehyde dehydrogenase 2 knockout mice treated with ethanol (2011)
  • Author(s): 小山倫浩
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(名古屋)(招待講演)
  • Year and Date: 2011-10-03
• [Presentation] エタノール摂取による症状とアルデヒド脱水素酵素(ALDH) 2多型の影響 (2010)
  • Author(s): 一瀬豊日
  • Organizer: 平成22年度アルコール・薬物依存関連学会合同学術総会
  • Place of Presentation: リーガロイヤルホテル小倉(北九州)
  • Year and Date: 2010-10-09
• [Presentation] エタノール摂取による症状とアルデヒド脱水素酵素(ALDH)2多型の影響 (2010)
  • Author(s): 一瀬豊日
  • Organizer: 平成22年度アルコール・薬物依存関連学会合同学術総会
  • Place of Presentation: リーガロイヤルホテル小倉(北九州)
  • Year and Date: 2010-10-09
• [Presentation] ダイオートアレイ検出器液体クロマトグラフィーによる感作性化学物質判定法 (2008)
  • Author(s): 一瀬豊日、小山倫浩、Pham Thi Thu PHoung、荻野景規、川本俊弘
  • Organizer: 第6回日本予防医学会学術総会
  • Place of Presentation: 東京医科大学病院(東京都新宿区)