Investigation of factors to enhance anaerobic benzene degradation by ecophysiological approach and application towards remediation of polluted sites

• Project/Area Number: 20681008
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Environmental technology/Environmental materials
• Research Institution: The University of Tokyo
• Principal Investigator: KURISU Futoshi The University of Tokyo, 大学院・工学系研究科, 准教授 (30312979)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥23,010,000 (Direct Cost: ¥17,700,000、Indirect Cost: ¥5,310,000); Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2009: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
• Keywords: 土壌汚染浄化 / ベンゼン / 安定同位体プローブ法 / 同位体トレーサー法 / MAR-FISH法

Research Abstract

As a low cost and low energy consumption treatment method for benzene pollution in soil and groundwater, anaerobic benzene degradation was studied to develop remediation process. Detection and quantification method were developed for benzene degrading bacteria Hasda-A by quantitative PCR and Fluorescent in situ hybridization. Enhancement of benzene degradation was tested and the benzene degradation was always coincided with Hasda-A growth. Degradation in groundwater from contaminated sites and simultaneous degradation with chlorinated ethylenes were also demonstrated.

Research Products

• [Journal Article] Identification of putative benzene-degrading bacteria in methanogenic enrichment cultures (2009)
  • Author(s): Sakai N, Kurisu F, Yagi O, Nakajima F, Yamamoto K
  • Journal Title: ournal of Bioscience and Bioengineering 108 Pages: 501-507
  • NAID: 110007503215
  • Peer Reviewed
• [Journal Article] Identification of putative benzene-degrading bacteria in methanogenic enrichment (2009)
  • Author(s): Sakai N, Kurisu F, Yagi O, Nakajima F, Yamamoto K
  • Journal Title: Journal of Bioscience and Bioengineering 108 Pages: 501-507
  • Peer Reviewed
• [Presentation] メタン生成ベンゼン分解集積培養系における微生物群集構造および機能解析 (2010)
  • Author(s): 對馬育夫, 舛本弘毅, 春日郁朗, 栗栖太, 古米弘明
  • Organizer: 第44回日本水環境学会年会
  • Place of Presentation: 福岡大学
  • Year and Date: 2010-03-17
• [Presentation] メタン生成嫌気集積培養系におけるベンゼン分解経路の同位体トレーサーを用いた検討 (2010)
  • Author(s): 舛本弘毅, 栗栖太, 對馬育夫, 春日郁朗, 古米弘明
  • Organizer: 第44回日本水環境学会年会
  • Place of Presentation: 福岡大学
  • Year and Date: 2010-03-17
• [Presentation] Hiroaki Furumai Methanogenic benzene degradation pathways examined by stable isotope tracers (2010)
  • Author(s): Futoshi Kurisu, Hiroki Masumoto, Ikuro Kasuga
  • Organizer: 13th International Symposium on Microbial Ecology, PS.07.057
  • Place of Presentation: シアトル(米国)
• [Presentation] Methanogenic benzene degradation pathways examined by stable isotope tracers (2010)
  • Author(s): Futoshi Kurisu, Hiroki Masumoto, Ikuro Kasuga, Hiroaki Furumai
  • Organizer: 13th International Symposium on Microbial Ecology, PS.07.057
  • Place of Presentation: シアトル(米国)
• [Presentation] 嫌気集積培養系によるベンゼンからのメタン生成の同位体トレーサーを用いた確認 (2009)
  • Author(s): 舛本弘毅, 栗栖太, 春日郁朗, 古米弘明
  • Organizer: 第25回日本微生物生態学会
  • Place of Presentation: 広島大学
  • Year and Date: 2009-11-21
• [Presentation] 複数土壌からの嫌気ベンゼン分解微生物群の集積と分解活性に及ぼす温度の影響 (2009)
  • Author(s): 舛本弘毅、栗栖太、春日郁朗、古米弘明
  • Organizer: 第43回日本水環境学会
  • Place of Presentation: 山口大学
  • Year and Date: 2009-03-17
• [Presentation] 複数土壌からの嫌気ベンゼン分解微生物群の集積と分解活性に及ぼす温度の影響 (2009)
  • Author(s): 舛本弘毅、栗栖太、春目郁朗、古米弘
  • Organizer: 第43回日本水環境学会
  • Place of Presentation: 山口大学(山口県)
  • Year and Date: 2009-03-17
• [Book] Bioremediation of Groundwater and Soil in Urban Areas. (Groundwater Management in Asian Cities-Technology and Policy for Sustainability)(In : Takizawa S.(ed.)) (2008)
  • Author(s): Kurisu F
  • Publisher: Springer