Development of degradable probes

• Project/Area Number: 20685012
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Moritoshi The University of Tokyo, 大学院・総合文化研究科, 准教授 (00323501)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥25,610,000 (Direct Cost: ¥19,700,000、Indirect Cost: ¥5,910,000); Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2008: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
• Keywords: プローブ / 可視化 / キナーゼ / ユビキチン / タンパク質分解 / タンパク質リン酸化 / 生物発光 / in vivo / イメージング / シグナル伝達

Research Abstract

Molecular imaging could be the most powerful technique for observing spatial and temporal dynamics of molecular processes in living cells and organisms, if probes for the relevant molecular processes become available. In the present study, we have developed degradable probes based on a new principle that allows imaging protein phosphorylation in living organisms.

Research Products

• [Journal Article] Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation by c-Jun NH_2-Terminal Kinase (JNK) in Living Cells (2010)
  • Author(s): H.Suzuki, M.Sato
  • Journal Title: Supramol.Chem. 22 Pages: 434-439
• [Journal Article] A Split Gaussia Luciferase-Based Bioluminescence Template for Tracing Protein Dynamics in Living Cells (2009)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Anal.Chem. 81 Pages: 67-74
• [Journal Article] Genetically Encoded Bioluminescent Indicators for Stress Hormones (2009)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Anal.Chem. 81 Pages: 3760-3768
• [Journal Article] Molecular Tension-Indexed Bioluminescent Probes for Determining Protein?Protein interactions (2009)
  • Author(s): S.B.Kim, M.Sato, H.Tao
  • Journal Title: Bioconjugate Chem. 20 Pages: 2324-2330
• [Journal Article] 分子プローブと細胞生命科学;見えなかったものを見えるようにする技術の開発 (2009)
  • Author(s): 佐藤守俊
  • Journal Title: 化学と工業 10月号 Pages: 1073-1075
  • NAID: 10025548538
• [Presentation] 分子イメージング (2011)
  • Organizer: 第4回学際融合ビジュアライゼーションシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2011-01-11
• [Presentation] 細胞内のシグナル伝達を可視化する分子プローブ (2010)
  • Organizer: 新学術領域「システム分子行動学」イメージングワークショップ
  • Place of Presentation: 博多
  • Year and Date: 2010-08-18
• [Presentation] 細胞内のシグナル伝達を可視化する分子プローブ (2010)
  • Organizer: 日本薬学会第130年会シンポジウム「薬学における生命志向型化学(分子設計を基盤とした生命現象解明)」
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] Imaging Molecular Processes in Living Cells (2009)
  • Organizer: Janelia Farm Conferecne on Fluorescent Proteins and Biological Sensors II
  • Place of Presentation: Virginia, USA
  • Year and Date: 2009-11-03
• [Presentation] 細胞内のシグナル伝達を可視化する分子プローブ (2009)
  • Organizer: 日本分析化学会第58年会化学センサー懇談会
  • Place of Presentation: 北海道
  • Year and Date: 2009-09-24
• [Presentation] Imaging Molecular Processes in Living Cells (2009)
  • Organizer: 2^<nd> Swiss-Japanese Symposium on Bionanotechnology BioNano 2009
  • Place of Presentation: Aigle Castle, Switzerland
  • Year and Date: 2009-09-11
• [Presentation] Fluorescent Indicators to Visualize Molecular Processes in Living Cells (2009)
  • Organizer: Cold Spring Harbor Laboratory Meeting on Single Cell Techniques
  • Place of Presentation: New York, USA
  • Year and Date: 2009-07-17
• [Remarks] ホームページ
  • URL: http://system.c.u-tokyo.ac.jp/common/professor/sato.html
• [Patent(Industrial Property Rights)] 超高輝度で安定な人工生物発光酵素 (2009)
  • Inventor(s): 金誠培, 佐藤守俊, 田尾博明
  • Industrial Property Rights Holder: 独立行政法人技術総合研究所,国立大学法人東京大学
  • Industrial Property Number: 2009-101025
  • Filing Date: 2009-04-16
• [Patent(Industrial Property Rights)] タンパク質の翻訳後修飾の検出・定量用プローブ (2009)
  • Inventor(s): 佐藤守俊,高橋浩治
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 2009-247098
  • Filing Date: 2009-10-27
• [Patent(Industrial Property Rights)] タンパク質の翻訳後修飾の検出・定量用プローブ (2009)
  • Inventor(s): 佐藤守俊, 高橋浩治
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 2009-247098
  • Filing Date: 2009-10-27