| Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2008: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Research Abstract |
Examining wastewater samples could be an effective approach to understand the actual prevalence and epidemiology of the viruses, since wastewater contains viruses shed from all populations, regardless of symptoms. In this study, we developed methodology and investigated the prevalence and genetic diversity of Norovirus and Aichivirus in wastewater.
Noroviruses were found abundantly in wastewater samples by RT-PCR methods, from which we succeeded in determining nucleic acid sequences by the cloning and sequencing method. Norovirus GI was more frequently and abundantly found from river water samples than Norovirus GII. This result implies that Norovirus GI was more popular than expected from epidemiological result. Aichivirus was also tested with similar method and revealed to be more diverse than expected previously.
In conclusion, we successfully detected targeted viruses from wastewater.
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