Chemical reactivity changes rely on microfluidic conformational changes

• Project/Area Number: 20686054
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Reaction engineering/Process system
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YAMASHITA Kenkichi National Institute of Advanced Industrial Science and Technology, ナノテクノロジー研究部門, 研究員 (90358250)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2008: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: 新規反応場 / 化学反応速度論 / 熱力学解析 / マイクロ流路 / 層流 / 酵素反応 / DNA / マイクロ流体 / マイクロリアクター

Research Abstract

This research revealed the capability of changing the activation energy and kinetic parameters of chemical reactions using microchannel laminar flow. Results show that the enhancing effect of microfluidics in molecular collision accelerated the chemical reaction. Results of activation energy analysis confirmed that a change in the apparent activation energy occurred as the flow rate changed. Taken together, these results suggest that a microfluidic system is useful for performing rapid reactions by influencing the activation energy and frequency of molecular collision.

Research Products

• [Journal Article] Nonimmobilized Enzyme kinetics that rely on laminar flow (2009)
  • Author(s): Kenichi Yamashita, Masaya Miyazaki, Hiroyuki Nakamura, Hideaki Maeda
  • Journal Title: The Journal of Physical Chemistry A Vol.113 Pages: 165-169
  • Peer Reviewed
• [Journal Article] Enhanced thermal stability and mismatch discrimination of mutation-carrying DNA duplexes and their kinetic and thermodynamic properties in microchannel laminar flow (2009)
  • Author(s): Nagata Maria Portia, Kenichi Yamashita, Masaya Miyazaki, Hiroyuki Nakamura, Hideaki Maeda
  • Journal Title: Analytical Biochemistry Vol.390 Pages: 38-45
  • Peer Reviewed
• [Journal Article] Enhanced thermal stability and mismatch discrimination of mutation-carrying DNA dunlexes and their kinetic and thermodvnamic nronerties in microchannel laminar flow (2009)
  • Author(s): 山下健一
  • Journal Title: Analytical Biochemistry 390 Pages: 38-45
  • Peer Reviewed
• [Journal Article] Nonimmobilized Enzyme Kinetics That Rely on Laminar Flow (2009)
  • Author(s): 山下健一
  • Journal Title: The Journal of Physical Chemistry A 113 Pages: 165-169
  • Peer Reviewed
• [Journal Article] The change of activation energy in microchannel laminar flow as demonstrated by kinetic analysis of the DNA duplex-coil equilibrium (2008)
  • Author(s): Kenichi Yamashita, Masaya Miyazaki, Hiroyuki Nakamura, Hideaki Maeda
  • Journal Title: Lab on a Chip Vol.8 Pages: 1171-1177
  • Peer Reviewed
• [Journal Article] The change of activation energy in microchannel laminar flow as demonstrated by kinetic analysis of the DNA duplex-coil equilibrium (2008)
  • Author(s): 山下健一
  • Journal Title: Lab on a Chip 8 Pages: 1171-1177
  • Peer Reviewed
• [Presentation] DNA鎖の形態変化と2本鎖形成・解離の速度論解析 (2008)
  • Author(s): 山下健一
  • Organizer: 第17回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 福岡市
  • Year and Date: 2008-05-21
• [Presentation] DNA鎖の形態変化と2本鎖形成・解離の速度論解析 (2008)
  • Author(s): 山下健一
  • Organizer: 第17回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 九州大学病院地区
  • Year and Date: 2008-05-21