Analysis of heterochromatin boundary function

• Project/Area Number: 20687001
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: University of Fukui
• Principal Investigator: OKI Masaya University of Fukui, 大学院・工学研究科, 准教授 (60420626)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥26,520,000 (Direct Cost: ¥20,400,000、Indirect Cost: ¥6,120,000); Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2008: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
• Keywords: ゲノム構築 / 再編 / 維持 / ヘテロクロマチン / バウンダリー / 出芽酵母 / ヒストン / サイレンシング / エピジェネティクス

Research Abstract

We analyzed the boundary which the extension of the heterochromatin domain stopped. Our result suggested that heterochromatin and proteasome had genetic interaction. In addition, we identified a novel histone binding protein and clarified the function domain in the living cell. I expect the function elucidation of the heterochromatin domain from the new viewpoint that is not reported conventionally in future by analyzing these genes more.

Research Products

• [Journal Article] Expression in Escherichia coli of an unnamed protein gene from Aspergillus oryzae RIB40 and cofactor analyses of the gene product as formate oxidase. (2009)
  • Author(s): Maeda Y, Dobayashi D, Oki M, Nose, Sakurai A, Isa K, Fujii Y, chida H.
  • Journal Title: Biosci. Biotechnol. Biochem. 73 Pages: 2645-2649
  • NAID: 10027548907
• [Journal Article] Expression in Escherichia coli of an unnamed protein gene from Aspergillus oryzae RIB40 and cofactor analyses of the gene product as formate oxidase. (2009)
  • Author(s): Yoshifumi Maeda
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 2645-2649
  • Peer Reviewed
• [Journal Article] Cloning and expression of three formate oxidase genes from Debaryomyces vanrijiae MH201. (2008)
  • Author(s): Maeda Y, Oki M, Fujii Y, Hatanaka A, Hojo M, Hirano K, Uchida H.
  • Journal Title: Biosci. Biotechnol. Biochem. 72 Pages: 1999-2004
  • NAID: 10027530599
• [Journal Article] Cloning and expression of three formate oxidase genes from Debaryomyces vanrijiae MH201 (2008)
  • Author(s): Yoshifumi Maeda
  • Journal Title: Biosci. Biotechnoi. Biochem 72 Pages: 1999-2004
  • Peer Reviewed
• [Presentation] 新たなアプローチからのエピジェネティクスの解明 (2010)
  • Author(s): 沖昌也
  • Organizer: 産業技術研究所講演会
  • Place of Presentation: 札幌
  • Year and Date: 2010-02-19
• [Presentation] 新たなアプローチからのエピジェネティクスの解明 (2010)
  • Author(s): 沖昌也
  • Organizer: (独)産業技術総合研究所 講演会
  • Place of Presentation: 札幌
  • Year and Date: 2010-02-19
• [Presentation] エピジェネティックな遺伝子発現切り替わりメカニズムの解析 (2010)
  • Author(s): 眞野恭伸、山口さやか、坂季美子、小林武彦、内田博之、沖昌也
  • Organizer: 第27回染色体ワークショップ
  • Place of Presentation: 御殿場
  • Year and Date: 2010-01-20
• [Presentation] Analysis of the heredity change of the boundary (2009)
  • Author(s): Yasunobu Mano, Akira Hatanaka, Hiroyuki Uchida, Masaya Oki
  • Organizer: Chromatin domains and Insulators
  • Place of Presentation: Baeza, Spain
  • Year and Date: 2009-11-10
• [Presentation] Analysis of the heredity change of the gene expression in the single cell (2009)
  • Author(s): Masaya Oki, Yasunobu Mano, Akira Hatanaka, Hiroyuki Uchida
  • Organizer: FASEB summer research conference
  • Place of Presentation: Colorado, USA
  • Year and Date: 2009-07-13
• [Presentation] 単一細胞におけるエピジェネティックな遺伝子発現切り替わりメカニズムの解析 (2009)
  • Author(s): 沖昌也, 眞野恭伸, 畑中彬良, 内田博之
  • Organizer: 第8回核ダイナミクス研究会
  • Place of Presentation: 伊豆
  • Year and Date: 2009-06-19
• [Presentation] 生体内におけるサイレンシング領域伸長停止メカニズム (2009)
  • Author(s): 畑中彬良、眞野恭伸、具雲峰、新名主カオリ、中山潤一、内田博之、沖昌也
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
• [Presentation] Analysis of heterochromatin barrier function in S. cerevisiae (2008)
  • Author(s): 沖昌也
  • Organizer: International Symposium on Chromosome Dynamics in Ise, 2008
  • Place of Presentation: 志摩
  • Year and Date: 2008-05-28
• [Presentation] ヘテロクロマチン領域伸長停止メカニズムの解析 (2008)
  • Author(s): 沖昌也
  • Organizer: 第2回日本エピジェネティクス研究会年会
  • Place of Presentation: 三島
  • Year and Date: 2008-05-10
• [Presentation] 生体内におけるサイレンシング領域伸長停止メカニズムの解析 (2008)
  • Author(s): 沖昌也
  • Organizer: 第26回染色体ワークショップ
  • Place of Presentation: 姫路
  • Year and Date: 2008-01-28
• [Presentation] Analysis of heterochromatin barrier function in S. cerevisiae (2008)
  • Author(s): Akira Hatanaka, Yasunobu Mano, Hiroyuki Uchida, Masaya Oki
  • Organizer: Gordon Research Conferences
  • Place of Presentation: Barga, Italy
• [Presentation] Analysis of heterochromatin barrier function in S. cerevisiae (2008)
  • Author(s): Masaya Oki
  • Organizer: International Symposium on Chromosome Dynamics
  • Place of Presentation: Ise, Japan
• [Presentation] ヘテロクロマチン領域伸長停止機能とTy因子の関与 (2008)
  • Author(s): 沖昌也、畑中彬良、野上惠美、内田博之
  • Organizer: 第31回日本分子生物学会
  • Place of Presentation: 神戸
• [Presentation] ヘテロクロマチン領域伸長停止メカニズムの解析 (2008)
  • Author(s): 沖昌也
  • Organizer: 第11回クロマチン研究会
  • Place of Presentation: 三島
• [Presentation] HMR left boundaryの機能解明 (2008)
  • Author(s): 沖昌也、畑中彬良、野上惠美、内田博之
  • Organizer: 第41回酵母遺伝学フォーラム
  • Place of Presentation: 札幌
• [Presentation] 生体内におけるヘテロクロマチン領域伸長停止メカニズムの解析 (2008)
  • Author(s): 沖昌也、畑中彬良、野上惠美、内田博之
  • Organizer: 第80回日本遺伝学会
  • Place of Presentation: 名古屋
• [Presentation] ヘテロクロマチン領域伸長停止メカニズムの解析 (2008)
  • Author(s): 畑中彬良、眞野恭伸、内田博之、沖昌也
  • Organizer: 第2回日本エピジェネティクス研究会年会
  • Place of Presentation: 静岡