Towards a molecular understanding of symbiotic mechanism of chemoautotrophs in deep-sea hydrothermal vent environments.

• Project/Area Number: 20687003
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Ecology/Environment
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NAKAGAWA Satoshi Japan Agency for Marine-Earth Science and Technology, 大学院・水産科学研究院, 准教授 (70435832)
• Co-Investigator(Renkei-kenkyūsha): TAKAI Ken 独立行政法人海洋研究開発機構, 研究員 (80359166) ; MAKITA Hiroko 独立行政法人海洋研究開発機構, 研究員 (40553219) ; SAWABE Tomoo 北海道大学, 大学院・水産科学研究院, 教授 (30241376)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
• Keywords: 深海底熱水孔環境 / 共生 / 生物間相互作用 / 糖鎖 / グライコミクス

Research Abstract

The main objective of this study is to reveal the molecular mechanisms underlying symbiont-host relationship in deep-sea hydrothermal vent environments. We have focused on sugar chains, glycans. We have succeeded in detection, profiling, structure determination of glycans for both symbionts and their host animals. Glycans having quite unique structures were identified.

Research Products

• [Journal Article] Hydrothermal fluid geochemistry at the Iheya North field in the mid-Okinawa Trough : Implication for origin of methane in subseafloor fluid circulation systems (2011)
  • Author(s): Kawagucci, S, Chiba, H, Ishibashi, J, Yamanaka, T, Toki, T, Muramatsu, Y, Ueno, Y, Makabe, A, Inoue, K, Yoshida, N, Nakagawa, S, Nunoura, T, Takai, K, Takahata, N, Sano, Y, Narita, T, Teranishi, G, Obata, H, Gamo, T
  • Journal Title: Geochem J 45 Pages: 109-124
  • NAID: 10028198287
  • Peer Reviewed
• [Journal Article] Hydrothermal fluid geochemistry at the Iheya North field in the mid-Okinawa Trough : Implication for origin of methane in subseafloor fluid circulation systems. (2011)
  • Author(s): Kawagucci, S.
  • Journal Title: Geochemical Journal Volume: (In press)
  • Peer Reviewed
• [Journal Article] Diversity and function of epibiotic microbial communities on the galatheid crab, Shinkaia crosnieri (2010)
  • Author(s): Watsuji, T, Nakagawa, S, Tsuchida, S, Toki, T, Hirota, A, Tsunogai, U, Takai, K
  • Journal Title: Microbes Environ 25 Pages: 288-294
  • NAID: 10027419964
  • Peer Reviewed
• [Journal Article] Bacterial Lifestyle in a Deep-sea Hydrothermal Vent Chimney Revealed by the Genome Sequence of the Thermophilic Bacterium Deferribacter desulfuricans SSM1 (2010)
  • Author(s): Takaki, Y, Shimamura, S, Nakagawa, S, Fukuhara, Y, Horikawa, H, Ankai, A, Harada, T, Hosoyama, A, Oguchi, A, Fukui, S, Fujita, N, Takami, H, Takai, K
  • Journal Title: DNA Res 17 Pages: 123-137
  • Peer Reviewed
• [Journal Article] Archaeoglobus sulfaticallidus sp.nov., a novel thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids. (2010)
  • Author(s): Steinsbu, BO, Thorseth, IH, Nakagawa, S, Inagaki, F, Lever, MA, Engelen, B, Ovreas, L, Pedersen, RB
  • Journal Title: Int J Syst Evol Microbiol 60 Pages: 2745-2752
  • Peer Reviewed
• [Journal Article] Diversity and function of epibiotic microbial communities on the galatheid crab, Shinkaia crosnieri. (2010)
  • Author(s): Watsuji, T.
  • Journal Title: Microbes and Environment Volume: 25 Pages: 288-294
  • Peer Reviewed
• [Journal Article] Archaeoglobus sulfaticallidus sp.nov., a novel thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids (2010)
  • Author(s): Steinsbu, B.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology (In press)
  • Peer Reviewed
• [Journal Article] Bacterial lifestyle in a deep-sea hydrothermal vent chimney revealed by the genome sequence of the thermophilic bacterium Deferribacter desul-furicans SSM1 (2010)
  • Author(s): Takaki, Y.
  • Journal Title: DNA Research (In press)
  • Peer Reviewed
• [Journal Article] Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney. (2009)
  • Author(s): Takai, K, Miyazaki, M, Hirayama, H, Nakagawa, S, Querellou, J, Godfroy, A
  • Journal Title: Environ Microbiol 11 Pages: 1983-1997
  • Peer Reviewed
• [Journal Article] Multi-probe-fluorescence in situ hybridization for the rapid enumeration of viable Vibrio parahaemolyticus. (2009)
  • Author(s): Sawabe, T, Yoshizawa, A, Kawanishi, Y, Komatsu, E, Nakagawa, S, Sawabe, T, Ootubo, M, Satomi, M, Yano, Y, Yamazaki, K
  • Journal Title: Microbes Environ 24 Pages: 259-264
  • NAID: 10025920896
  • Peer Reviewed
• [Journal Article] Nonpathogenic Epsilonproteobacteria. (2009)
  • Author(s): Nakagawa, S, Takaki, Y
  • Journal Title: Encyclopedia of Life Sciences a002189
  • Peer Reviewed
• [Journal Article] Multi-probe-fluorescence in situ hybridization for the rapid enumeration of viable Vibrio parahaemolyticus (2009)
  • Author(s): Sawabe, T.
  • Journal Title: Microbes and Environment 24 Pages: 259-264
  • NAID: 10025920896
  • Peer Reviewed
• [Journal Article] Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney (2009)
  • Author(s): Takai, K.
  • Journal Title: Environmental Microbiology 11 Pages: 1983-1997
  • Peer Reviewed
• [Journal Article] Non-pathogenic epsilon-Proteobacteria. (2009)
  • Author(s): Nakagawa, S.
  • Journal Title: Encyclopedia of Life Sciences (In press)
  • Peer Reviewed
• [Journal Article] Exilispira thermophila gen.nov., sp.nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney. (2008)
  • Author(s): Imachi, H, Sakai, S, Hirayama, H, Nakagawa, S, Nunoura, T, Takai, K, Horikoshi, K
  • Journal Title: Int J Syst Evol Microbiol 58 Pages: 2258-2265
  • Peer Reviewed
• [Journal Article] Cell proliferation at 122℃ and isotopically heavy CH_4 production by a hyperthermophilic methanogen under high-pressure cultivation. (2008)
  • Author(s): Takai, K, Nakamura, K, Toki, T, Tsunogai, U, Miyazaki, M, Miyazaki, J, Hirayama, H, Nakagawa, S, Nunoura, T, Horikoshi, K
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 10949-10954
  • Peer Reviewed
• [Journal Article] Deep-sea vent chemoautotrophs : diversity, biochemistry and ecological significance. (2008)
  • Author(s): Nakagawa, S, Takai, K
  • Journal Title: FEMS Microbiol Ecol 65 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Deep-sea vent chemoautotrophs: diversity, biochemistry and ecological significance. (2008)
  • Author(s): Nakagawa, S.
  • Journal Title: FEMS Microbiology Ecology 65 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Cell proliferation at 122 ℃ and isotopically heavy CH_4 production by a hyperthermophilic methanogen under high-pressure cultivation. (2008)
  • Author(s): Takai, K.
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America 105 Pages: 10949-10954
  • Peer Reviewed
• [Journal Article] Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete from a deep-sea hydrothermal vent chimney. (2008)
  • Author(s): Imachi, H.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 58 Pages: 2258-2265
  • Peer Reviewed
• [Presentation] インド洋の深海底熱水活動域に棲息する細胞内共生微生物のゲノム解析 (2011)
  • Author(s): 中川聡
  • Organizer: ブルーアース11
  • Place of Presentation: 東京海洋大学品川キャンパス(東京都品川区)
  • Year and Date: 2011-03-08
• [Presentation] インド洋の深海底熱水活動域に棲息する細胞内共生微生物のゲノム解析 (2011)
  • Author(s): 中川聡
  • Organizer: ブルーアース11
  • Place of Presentation: 東京海洋大学(東京都港区)
  • Year and Date: 2011-03-08
• [Presentation] インド洋の深海底熱水活動域に棲息する細胞内共生微生物のゲノム解析 (2010)
  • Author(s): 中川聡
  • Organizer: 第26回微生物生態学会大会
  • Place of Presentation: 筑波大学大学会館(つくば市)
  • Year and Date: 2010-11-25
• [Presentation] 海藻糖質をエタノール-水素発酵するビブリオの種同定 (2010)
  • Author(s): 澤辺智雄
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 日本大学生物資源科学部(藤沢市)
  • Year and Date: 2010-03-28
• [Presentation] 海藻糖質をエタノール-水素発酵するビブリオの種同定 (2010)
  • Author(s): 澤辺智雄
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2010-03-28
• [Presentation] 魚介類の粘液に対する腸炎ビブリオの化学走性 (2009)
  • Author(s): 石澤明子
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 岡山大学大学院自然科学研究科(岡山市)
  • Year and Date: 2009-11-26
• [Presentation] 魚介類の粘液に対する腸炎ビブリオの化学走性 (2009)
  • Author(s): 石澤明子
  • Organizer: 腸炎ビブリオシンポジウム
  • Place of Presentation: 岡山大学大学院自然科学研究科
  • Year and Date: 2009-11-26
• [Presentation] Chemotaxis of Vibrio harveyi using sequence type defined strains. (2009)
  • Author(s): Sawabe, T
  • Organizer: Vibrio 2009
  • Place of Presentation: リオデジャネイロ・ブラジル
  • Year and Date: 2009-11-06
• [Presentation] Chemotaxis of Vibrio harveyi using sequence type defined strains (2009)
  • Author(s): 澤辺智雄
  • Organizer: Vibrio 2009
  • Place of Presentation: リオデジャネイロ(ブラジル)
  • Year and Date: 2009-11-06
• [Presentation] 深海底熱水活動域に優占する化学合成微生物の群集遺伝学的解析 (2009)
  • Author(s): 中川聡
  • Organizer: ブルーアース2009
  • Place of Presentation: 立教大学(東京都豊島区)
  • Year and Date: 2009-03-13
• [Presentation] 深海底熱水活動域に優占する化学合成微生物の群集遺伝学的解析 (2009)
  • Author(s): 中川聡
  • Organizer: ブルーアース2009
  • Place of Presentation: 立教大学
  • Year and Date: 2009-03-13
• [Presentation] Allelic diversity and population structure in deep-sea vent epsilon-Proteobacteria as determined from sequence analysis of housekeeping genes. (2009)
  • Author(s): Nakagawa, S
  • Organizer: ASLO2009
  • Place of Presentation: ニース・フランス
  • Year and Date: 2009-01-28
• [Presentation] Allelic diversity and population structure in deep-sea vent epsilon-Prote obacteria as determinied from sequence analysis of housekeeping genes. (2009)
  • Author(s): Nakagawa, S.
  • Organizer: ASLO2009
  • Place of Presentation: ニース
  • Year and Date: 2009-01-28
• [Presentation] Genome analysis of deep-sea vent epsilon-Proteobacteria. (2008)
  • Author(s): Nakagawa, S
  • Organizer: ISSM Satellite Symposium
  • Place of Presentation: 中央大学(東京都文京区)
  • Year and Date: 2008-11-15
• [Presentation] Genome analysis of deep-sea vent epsilon-Proteobacteria. (2008)
  • Author(s): Nakagawa, S.
  • Organizer: ISSM Satellite Symposium
  • Place of Presentation: 中央大学
  • Year and Date: 2008-11-15
• [Presentation] Population genetic analysis of deep-sea vent epsilon-Proteobacteria by multilocus sequence typing. (2008)
  • Author(s): Nakagawa, S
  • Organizer: ISME12
  • Place of Presentation: ケアンズ・オーストラリア
  • Year and Date: 2008-08-21
• [Presentation] Population genetic analysis of deep-sea vent epsilon-Proteobacteria by multilocus sequence typing. (2008)
  • Author(s): Nakagawa, S.
  • Organizer: ISME12
  • Place of Presentation: ケアンズ、オーストリア
  • Year and Date: 2008-08-21
• [Remarks]
  • URL: http://www.hokudai.ac.jp/bureau/topics/press_release/091130_pr_scaly-foot.pdf
• [Patent(Industrial Property Rights)] アルギン酸の分解方法 (2010)
  • Inventor(s): 井上晶、中川聡
  • Industrial Property Rights Holder: 北海道大学
  • Industrial Property Number: 2010-065945
  • Filing Date: 2010-03-23