Structural basis of vesicle formation in endocytosis

• Project/Area Number: 20687006
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Kyushu University (2011); The Institute of Physical and Chemical Research (2008-2010)
• Principal Investigator: SHIMADA Atsushi 九州大学, 生体防御医学研究所, 准教授 (70391977)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000); Fiscal Year 2011: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: GTP結合タンパク質 / X線結晶構造解析 / アクチン細胞骨格 / エンドサイトーシス / 細胞内シグナル伝達 / 脂質膜 / X線結晶解析

Research Abstract

Endocytosis is a process by which eukaryotic cells internalize extracellular materials into the cell. This process plays an important role in a variety of fundamental physiological responses, such as cell surface receptor internalization and somatic nutrient uptake. In this project, we performed structural and functional analyses of endocytic proteins, such as synaptojanin, PACSIN2/Syndapin II, CIP4, FBP17, Cdc42, and WAVE2 complex, and revealed the structural bases of various aspects of vesicle formation in endocytosis.

Research Products

• [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II (2010)
  • Author(s): Atsushi Shimada, Kazunori Takano, Mikako Shirouzu, Kyoko Hanawa-Suetsugu, Takaho Terada, Kiminori Toyooka, Takashi Umehara, Masaki Yamamoto, Shigeyuki Yokoyama, and Shiro Suetsugu
  • Journal Title: FEBS Letters Volume: 584巻 Pages: 1111-1118
  • Peer Reviewed
• [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II (2010)
  • Author(s): Atsushi Shimada, Kazunori Takano, Mikako Shirouzu, Kyoko Hanawa-Suetsugu, Takaho Terada, Kiminori Toyooka, Takashi Umehara, Masaki Yamamoto, Shigeyuki Yokoyama, Shiro Suetsugu
  • Journal Title: FEBS Letters 584巻 Pages: 1111-1118
  • Peer Reviewed
• [Journal Article] Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II
  • Author(s): Atsushi Shimada, Kazunori Takano, Mikako Shirouzu, Kyoko Hanawa-Suetsugu, Takaho Terada, Kiminori Toyooka, Takashi Umehara, Masaki Yamamoto, Shigeyuki Yokoyama, Shiro Suetsugu
  • Journal Title: FEBS Letters (in press)
  • Peer Reviewed
• [Presentation] Structural basis of Cdc42-mediated Cdc42-interacting protein 4(CIP4) recruitment to clathrin-coated pits (2012)
  • Author(s): Atsushi Shimada
  • Organizer: The 21st Hot SPring Harbor Symposium jointly with 9th Global-COE International Symposium Cell Migration in Biology and Medicine-8th Young Investigators Forum-
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2012-01-21
• [Presentation] Structural basis of Cdc42-mediated Cdc42-interacting protein 4 (CIP4) recruitment to clathrin-coated pits (2012)
  • Author(s): Atsushi Shimada
  • Organizer: The 21^<st> Hot Spring Harbor Symposium jointly with 9^<th> Global-COE International Symposium Cell Migration in Biology and Medicine-8^<th> Young Investigators Forum-
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2012-01-21
• [Presentation] Cdc42によるCdc42-interacting protein 4(CIP4)のクラスリン被覆ピットへの局在機構の構造的基盤 (2011)
  • Author(s): 嶋田睦
  • Organizer: 日本生物物理学会九州支部例会
  • Place of Presentation: 福岡県飯塚市
  • Year and Date: 2011-12-04
• [Presentation] Cdc42によるCdc42-interacting protein 4 (CIP4)のクラスリン被覆ピットへの局在機構の構造的基盤 (2011)
  • Author(s): 嶋田睦
  • Organizer: 日本生物物理学会九州支部例会
  • Place of Presentation: のがみプレジデントホテル(福岡県飯塚市)
  • Year and Date: 2011-12-04
• [Presentation] BARドメインスーパーファミリータンパク質による脂質膜変形機構の構造的基盤 (2011)
  • Author(s): 嶋田睦,山本雅貴,横山茂之
  • Organizer: 大阪大学蛋白質研究所セミナー細胞表面受容体と細胞内輸送
  • Place of Presentation: 大阪
  • Year and Date: 2011-01-13
• [Presentation] BARドメインスーパーファミリータンパク質による脂質膜変形機構の構造的基盤 (2011)
  • Author(s): 嶋田睦, 山本雅貴, 横山茂之
  • Organizer: 大阪大学蛋白質研究所セミナー 細胞表面受容体と細胞内輸送
  • Place of Presentation: 大阪
  • Year and Date: 2011-01-13
• [Presentation] Structural basis of Cdc42-mediated Cdc42-interacting protein 4(CIP4) recruitment to clathrin-coated pits (2011)
  • Author(s): Atsushi Shimada
  • Organizer: 10th Global-COE International Symposium and 7th Young Investigators Forum
  • Place of Presentation: シンガポール
• [Presentation] Structural basis of Cdc42-mediated Cdc42 interacting protein 4 (CIP4) recruitment to clathrin-coated pits (2011)
  • Author(s): Atsushi Shimada
  • Organizer: 10^<th> Global-COE International Symposium and 7^<th> Young Investigators Forum
  • Place of Presentation: Singapore
• [Presentation] EFC/F-BARドメインタンパク質の構造,機能と制御 (2009)
  • Author(s): 嶋田睦,寺田貴帆,白水美香子,山本雅貴,永山國昭,末次志郎,竹縄忠臣,横山茂之
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島県徳島市
• [Presentation] EFC/F-BARドメインタンパク質の構造 機能と制御 (2009)
  • Author(s): 嶋田睦, 寺田貴帆, 白水美香子, 山本雅貴, 永山國昭, 末次志郎, 竹縄忠臣, 横山茂之
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島
• [Presentation] EFC/F-BARドメインタンパク質の構造、機能と制御 (2009)
  • Author(s): 嶋田睦、寺田貴帆、白水美香子、山本雅貴、永山國昭、末次志郎、竹縄忠臣、横山茂之
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島文理大学
• [Presentation] Cdc42-interacting protein 4(CIP4)によるCdc42認識機構の構造的基盤 (2008)
  • Author(s): 嶋田睦,外山光俊,寺田貴帆,田仲昭子,菅野純夫,白水美香子,山本雅貴,末次志郎,竹縄忠臣,横山茂之
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 兵庫県神戸市
• [Presentation] Cdc42-interacting protein4(CIP4)によるCdc42認識機構の構造的基盤 (2008)
  • Author(s): 嶋田睦, 外山光俊, 寺田貴帆, 田仲昭子, 菅野純夫, 白水美香子, 山本雅貴, 末次志郎, 竹縄忠臣, 横山茂之
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
• [Presentation] Cdc42-interacting protein 4 (CIP4) によるCdc42認識機構の構造的基盤 (2008)
  • Author(s): 嶋田睦
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://www.bioreg.kyushu-u.ac.jp/vsb/index.html