Elucidation of reaction mechanism for post-transcriptional modification at tRNA anticodon region
Project/Area Number |
20687007
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Research Category |
Grant-in-Aid for Young Scientists (A)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
NUMATA Tomoyuki National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 研究員 (10401564)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
|
Keywords | tRNA / 転写後修飾 / RNA修飾酵素 / 結晶構造解析 / 揺らぎ塩基 / アンチコドン / 古細菌 / ポリアミン / ミトコンドリア病 / 遺伝暗号翻訳 / アンチコト゛ン |
Research Abstract |
The uridine at the first position of the tRNA anticodon (U34) in tRNA^<Glu>UUC, tRNA^<Lys>UUU, tRNA^<Gln>UUG is modified to 5-carboxymethylaminomethyl-2-thiouridine (cmnm^5s^2U), which is crucial for the precise decoding of the genetic code. In eubacteria, two conserved proteins, GidA and MnmE, are responsible for adding the cmnm5 group to the 5-position of U34. Here we report the crystal structures of GidA and MnmE. Together with the mutational study, we propose a novel mechanism for the cmnm^5U modification process.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea.2010
Author(s)
Ikeuchi, Y., Kimura, S., Numata, T., Nakamura, D., Yokogawa, T., Ogata, T., Wada, T., Suzuki, T., Suzuki, T.
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Journal Title
Nat.Chem.Biol. 6
Pages: 277-282
Related Report
Peer Reviewed
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[Journal Article] Conserved cysteine residues of GidA are essential for biogenesis of 5-carboxymethylaminomethyluridine at tRNA anticodon.2009
Author(s)
Osawa, T., Ito, K., Inanaga, H., Nureki, O., Tomita, K., Numata, T.
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Journal Title
Structure* : Corresponding author 17
Pages: 713-724
Related Report
Peer Reviewed
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