| Budget Amount *help |
¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000)
Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2008: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Research Abstract |
The uridine at the first position of the tRNA anticodon (U34) in tRNA^<Glu>UUC, tRNA^<Lys>UUU, tRNA^<Gln>UUG is modified to 5-carboxymethylaminomethyl-2-thiouridine (cmnm^5s^2U), which is crucial for the precise decoding of the genetic code. In eubacteria, two conserved proteins, GidA and MnmE, are responsible for adding the cmnm5 group to the 5-position of U34. Here we report the crystal structures of GidA and MnmE. Together with the mutational study, we propose a novel mechanism for the cmnm^5U modification process.
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