Elucidation of the mechanism of intracellular phosphatidylinositol trafficking by the Phs1 family
| Project/Area Number |
20687008
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIHARA Akio Hokkaido University, 大学院・薬学研究院, 教授 (50333620)
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| Co-Investigator(Renkei-kenkyūsha) |
SASSA Takayuki 北海道大学, 大学院・薬学研究院, 講師 (20342793)
OBARA Keisuke 北海道大学, 大学院・薬学研究院, 助教 (30419858)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | 脂質 / 代謝 / 細胞内輸送 / 脂肪酸 / 極長鎖脂肪酸 / スフィンゴ脂質 / 遺伝子 / タンパク質 / ホスファチジルイノシトール / 脂質輸送 / 生体膜 / 縮合酵素 / アシルCoA / 3-ヒドロキシアシルCoA脱水酵素 |
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| Research Abstract |
Phs1 is a 3-hydroxyacyl-CoA dehydratase involved in the elongation of very long-chain fatty acids (VLCFAs). We demonstrated here that Phs1 regulates sphingolipid synthesis and phosphatidylinositol transport through interacting with proteins involved in lipid metabolism and vesicular transport. We also succeeded in identifying mammalian homologs of Phs1, HACD1-4. By revealing substrate specificities of enzymes catalyzing the rate-limiting step of the VLCFA elongation, we have elucidated the entire VLCFA pathways in mammals. Moreover, we obtained some knowledge about relationship between VLCFA elongation and certain disorders.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis.2010
Author(s)
Ohno, Y., Suto, S., Yamanaka, M., Mizutani, Y., Mitsutake, S., Igarashi, Y., Sassa, T., Kihara, A.
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Journal Title
Proc.Natl.Acad.Sci.USA 107
Pages: 18439-18444
NAID
Related Report
Peer Reviewed
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[Journal Article] Hetero-oligomeric interactions of an ELOVL4 mutant protein : implications in the molecular mechanism of Stargardt-3 macular dystrophy.2010
Author(s)
Okuda, A., Naganuma, T., Ohno, Y., Abe, K., Yamagata, M., Igarashi, Y., Kihara, A.
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Journal Title
Mol.Vis. 16
Pages: 2438-2445
Related Report
Peer Reviewed
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[Journal Article] Membrane topology and essential amino acid residues of Phs1, a 3-hydroxyacyl-CoA dehydratase involved in very long-chain fatty acid elongation.2008
Author(s)
Kihara, A., Sakuraba, H., Ikeda, M., Denpoh, A., Igarashi, Y.
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Journal Title
J.Biol.Chem. 283
Pages: 11199-11209
Related Report
Peer Reviewed
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[Journal Article] Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis.2008
Author(s)
Ikeda, M., Kanao, Y., Yamanaka, M., Sakuraba, H., Mizutani, Y., Igarashi, Y., Kihara, A.
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Journal Title
FEBS Lett. 582
Pages: 2435-2440
Related Report
Peer Reviewed
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