Elucidation of mechanism of AAA-type molecular motors, based on structural analysis of the dynein heavy chain

• Project/Area Number: 20687011
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: The University of Tokyo
• Principal Investigator: KON Takahide The University of Tokyo, 蛋白質研究所, 准教授 (30332620)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000); Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000); Fiscal Year 2009: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2008: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
• Keywords: 分子モーター / ナノマシン / 酵素反応 / 生物物理学 / 構造生物学 / 生物物理 / ナノバイオ / ダイニン

Research Abstract

Dyneins are enormous motor complexes that power a wide variety of cellular motility. However, the mechanism by which dynein generates force and movement still remains unclear. Here, we have succeeded in crystallizing dynein molecules and obtained dynein's structural information at 5 Å resolution. This study provides a structural basis for understanding the mechanism of action of the dynein motor.

Research Products

• [Journal Article] C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulation (2011)
  • Author(s): Numata, N., Shima, T., Ohkura, R., Kon, T., Sutoh, K.
  • Journal Title: FEBS Lett. 585 Pages: 185-1190
  • Peer Reviewed
• [Journal Article] C-sequence of the Dictyostelium cytoplasmic dynein participates in processivity modulation (2011)
  • Author(s): Numata, N., Shima, T., Ohkura, R., Kon, T., Sutoh, K.
  • Journal Title: FEBS Letters Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] 細胞質ダイニンの構造と機能のモジュール性 (2011)
  • Author(s): 島知弘, 須藤和夫, 昆隆英
  • Journal Title: 生物物理 Volume: 51-3(掲載確定)
  • NAID: 10029139922
  • Peer Reviewed
• [Journal Article] Protein engineering approaches to study the dynein mechanism using a Dictyostelium expression system. (2009)
  • Author(s): Kon, T., Shima, T., Sutoh, K.
  • Journal Title: Methods Cell Biol. 92 Pages: 65-82
• [Journal Article] Helix sliding in the stalk coiled coil of dynein couples ATPase and microtubule binding. (2009)
  • Author(s): Kon, T., Imamula, K., Roberts, A.J., Ohkura, R., Knight, P.J., Gibbons, I.R., Burgess, S.A., Sutoh, K.
  • Journal Title: Nature Struct.Mol.Biol. 16(3) Pages: 325-333
  • Peer Reviewed
• [Journal Article] AAA+ring and linker swing mechanism in the dynein motor. (2009)
  • Author(s): Roberts, A.J., Numata, N., Walker, M.L., Malkova, B., Kon, T., Ohkura, R., Arisaka, F., Knight, P.J., Sutoh, K., Burgess, S.A.
  • Journal Title: Cell. 136(3) Pages: 485-495
  • Peer Reviewed
• [Journal Article] Protein engineering approaches to study the dynein mechanism using a Dictyostelium expression system (2009)
  • Author(s): Kon, T., Shima, T., Sutoh, K.
  • Journal Title: Methods Cell Biol. 92 Pages: 65-82
• [Journal Article] Helix sliding in the stalk coiled coil of dynein couples ATPase and microtubule binding (2009)
  • Author(s): Kon, T., Imamula, K., Roberts, A.J., Ohkura, R., Knight, P.J., Gibbons, I.R.. Burgess. S.A.. Sutoh. K.
  • Journal Title: Nature Struct. Mel Biol. 16 Pages: 325-333
  • Peer Reviewed
• [Journal Article] AAA+ ring and linker swing mechanism in the dynein motor (2009)
  • Author(s): Roberts, A.J., Numata, N., Walker, M.L., Malkova, B., Kon, T., Ohkura, R., Arisaka, F., Knight, P.J., Sutoh, K., Burgess, S.A.
  • Journal Title: Cell 136 Pages: 485-495
  • Peer Reviewed
• [Presentation] Crystal Structure of the Dynein Motor Domain at 5 A Resolution (2011)
  • Author(s): Takahide Kon
  • Organizer: New and Notable Symposium at the Biophysical Society 55th Annual Meeting(招待講演)
  • Place of Presentation: in Baltimore, Maryland.
  • Year and Date: 2011-03-06
• [Presentation] Crystal Structure of the Dynein Motor Domain at 5 A Resolution (2011)
  • Author(s): Takahide Kon
  • Organizer: Biophysical Society 55th Annual Meeting.
  • Place of Presentation: Baltimore convention center (USA)(招待講演)
  • Year and Date: 2011-03-06
• [Presentation] ATPase kinetics and processivity of cytoplasmic dynein (2010)
  • Author(s): Takahide Kon
  • Organizer: International Symposium on Single Molecule Nano Detection and Its Application to Life Science(招待講演)
  • Place of Presentation: Awaji Yumebutai International Conference Center.
  • Year and Date: 2010-04-17
• [Presentation] ATPase kinetics and processivity of cytoplasmic dynein (2010)
  • Author(s): Takahide Kon
  • Organizer: International Symposium on Single Molecule Nano Detection and Its Application to Life Science
  • Place of Presentation: Awaji Yumebutai International Conference Center(招待講演)
  • Year and Date: 2010-04-16
• [Presentation] 細胞性粘菌を用いたダイニンの運動メカニズム研究 (2010)
  • Author(s): 昆隆英
  • Organizer: 日本農芸化学会2010年度大会(招待講演)
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-30
• [Presentation] 細胞性粘菌を用いたダイニンの運動メカニズム研究 (2010)
  • Author(s): 昆隆英
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-03-30
• [Presentation] Dissection of intramolecular communications between the catalytic head and microtubule-binding domains in the dynein heavy chain (2009)
  • Author(s): Takahide Kon
  • Organizer: ional Workshop Dynein 2009.(招待講演)
  • Place of Presentation: 神戸ファッション美術館オルビスホール
  • Year and Date: 2009-11-02
• [Presentation] DISSECTION OF INTRAMOLECULAR COMMUNICATIONS BETWEEN THE CATALYTIC HEAD AND MICROTUBULE-BINDING DOMAINS IN THE DYNEIN HEAVY CHAIN (2009)
  • Author(s): 昆隆英
  • Organizer: International Workshop Dynein 2009
  • Place of Presentation: 神戸ファッション美術館オルビスホール
  • Year and Date: 2009-11-02
• [Presentation] Molecular dissection of cytoplasmic dynein (2008)
  • Author(s): Takahide Kon
  • Organizer: 第46回日本生物物理学年会
  • Place of Presentation: 福岡国際会議場(口頭発表)
  • Year and Date: 2008-12-04
• [Presentation] Molecular dissection of cytoplasmic dynein (2008)
  • Author(s): 昆隆英
  • Organizer: 第46回日本生物物理学年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2008-12-04
• [Presentation] Structure and mechanochemical cycle of cytoplasmic dynein (2008)
  • Author(s): Takahide Kon
  • Organizer: 2nd International Symposium on Bio-nanosystems. Koshiba Hall the University of Tokyo(招待講演)
  • Place of Presentation: Hongo, Tokyo, Japan.
  • Year and Date: 2008-11-01
• [Presentation] Structure and mechanochemical cycle of cytoplasmic dynein (2008)
  • Author(s): 昆隆英
  • Organizer: 2nd International Symposium on Bio-nanosystems
  • Place of Presentation: 東京大学小柴ホール
  • Year and Date: 2008-11-01
• [Book] Handbook of Dynein (2011)
  • Author(s): Shima, T., Sutoh, K., Kon, T.(Amos, L, Hirose, K., Eds)
  • Publisher: Pan Stanford Publishing Pte Ltd.(印刷中)
• [Book] In Handbook of Dynein (Amos, L and Hirose, K., Eds), Pan Stanford Publishing Pte Ltd.
  • Author(s): Shima, T., Sutoh, K., Kon, T.
  • Publisher: Functional analysis of the dynein motor domain.(In press.)