| Budget Amount *help |
¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2008: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Research Abstract |
We analyzed a phosphate translocation mechanism of arbuscular mycorrhiza by using methods of immunocytochemistry and molecular biology. Polyphosphates were detected in vacuoles and cell walls of intraradical hyphae, but were hardly detected in arbuscules. As an acid phosphatase activity was detected in peri-arbuscular spaces, the polyphosphate in cell wall of arbuscules may be hydrolyzed by the acid phosphatase. We also identified a purple acid phosphatase gene LjPAP3 in Lotus japonicus, which specifically expressed in cortical cells including arbuscules.
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