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耳石の微量元素組成と安定同位体組成を指標とした魚類の回遊と集団構造の解析

Research Project

Project/Area Number 20688008
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

新井 崇臣  The University of Tokyo, 海洋研究所, 准教授 (70323631)

Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2008: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
Keywords耳石 / 微量元素 / 系群判別 / 回遊履歴 / LA-ICPMS / EPMA / 回遊魚 / 多様性 / ニシン / 通し回遊魚
Research Abstract

本年度は、レーザーアブレーション高周波誘導結合プラズマ質量分析法や波長分散型X線分析装置を用いて魚類耳石中の微量元素を多元種同時に測定し、水産重要種であるニシンやウナギなどの回遊魚の解析を行った。ニシンの飼育は、水温一定(13℃)で塩分を変化させた3つの実験群(11,23,35‰)、塩分一定(23‰)で水温を変化させた3つの実験群(9,13,17℃)で行なった。ニシンは、孵化から60日間飼育し、これを標本とした。耳石中の微量元素組成(Na/Ca,Mg/Ca,K/Ca,Sr/Ca,Ba/Ca)は、レーザーアブレーション誘導結合プラズマ質量分析法(LA-ICP-MS)を用いて測定した。得られた耳石中の元素組成と、水温および塩分との関係性は、分散分析法および相関係数を用いて解析した。水温を変化させた実験群間においては、測定した全ての元素カルシウム比において有意差が見出された。一方、塩分を変化させた実験群間においては、K/Ca、Sr/CaおよびBa/Ca比で有意な差が見出された。特に、Sr/Ca比と塩分は、強い正の相関関係を示した。以上の結果から、ニシン耳石中の元素組成は、水温と塩分から影響を受けるが、その影響の程度は元素によって異なることが示唆された。インドネシアジャワ島で採集された熱帯ウナギA.bicolor bicolorを用いて回遊履歴に推定を行ったところ、4つの回遊型が見いだされた。このことから、インドネシアに生息する本種は、多様な回遊生態を有することが明らかになった。

Report

(2 results)
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (17 results)

All 2010 2009 2008

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (8 results)

  • [Journal Article] Migratory history of the giant mottled eel(Anguilla marmorata)in the Bonin Islands of Japan2010

    • Author(s)
      Chino N, Arai T
    • Journal Title

      Ecology of Freshwater Fish 19

      Pages: 19-25

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of marine resident tropical eel Anguilla bicolor bicolor in Indonesia2010

    • Author(s)
      Chino N, Arai T
    • Journal Title

      Marine Biology 157

      Pages: 1075-1081

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Migratory histories of Rhinogobius sp.OR morphotype "Shimahire" as revealed by otolith Sr : Ca ratios2010

    • Author(s)
      Tsunagawa T, Suzuki T, Arai T
    • Journal Title

      Ichthyological Research 57

      Pages: 10-15

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of estuarine and sea eels Anguilla japonica and a migrating silvereel Anguilla anguilla in Tokyo Bay area, Japan2009

    • Author(s)
      Arai T, Chino N, Kotake A
    • Journal Title

      Fisheries Science 75

      Pages: 1197-1203

    • NAID

      10025406832

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relative contribution of migratory type on the reproduction of migrating silvereels, Anguilla japonica, collected off Shikoku Island, Japan2009

    • Author(s)
      Chino N, Arai T
    • Journal Title

      Marine Biology 156

      Pages: 661-668

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Migration diversity of the freshwater goby Rhinogobius sp.BI, as revealed by otolith Sr : Ca ratios2009

    • Author(s)
      Tsunagawa T, Arai T
    • Journal Title

      Aquatic Biology 5

      Pages: 187-194

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diverse migratory histories in a brackish water type of the ninespine stickleback, Punzitius punkitius2008

    • Author(s)
      Arai T, Goto A
    • Journal Title

      Environmental Biology of Fishes 83

      Pages: 349-353

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variation in migratory history of Japanese eels, Anguilla japonica, collected in the northernmost part of its distribution2008

    • Author(s)
      Arai T, Kotake A, Ohji M
    • Journal Title

      Journal of the Marine Biological Association of the United Kingdom 88

      Pages: 1075-1080

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Flexible migration of Japanese freshwater gobies Rhinogobius spp. as revealed by otolith Sr : Ca ratios2008

    • Author(s)
      Tsunagawa T, Arai T
    • Journal Title

      Journal of Fish Biology 73

      Pages: 2421-2433

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 神奈川県三浦半島で採集されたウナギ Anguilla japonica の回遊履歴2009

    • Author(s)
      茅野尚子, 新井崇臣
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報交流センター・アイーナ
    • Year and Date
      2009-10-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] 三陸沿岸域に生息するウナギの多様な回遊生態2009

    • Author(s)
      茅野尚子, 新井崇臣
    • Organizer
      東京大学海洋研究所共同利用シンポジウム大槌湾の生態系保全と持続的な利用を目指した研究の現状と展望
    • Place of Presentation
      大槌町中央公民館
    • Year and Date
      2009-08-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Comparison of habitat use between temperate and tropical freshwater eels of the genus Anguilla2009

    • Author(s)
      茅野尚子, 新井崇臣
    • Organizer
      Fourth International Symposium on Fish Otolith Research and Application
    • Place of Presentation
      Monterey, USA
    • Year and Date
      2009-08-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Assessing the usefulness of pressed powder pellet standard prepared from three biogenic calcium carbonate certified reference material for improving the accuracy of otolith chemistry using LA-ICP-MS2009

    • Author(s)
      Shirai K, Yamane K, Amano Y, Arai T, Otake T
    • Organizer
      Fourth International Symposium on Fish Otolith Research and Application
    • Place of Presentation
      Monterey, USA
    • Year and Date
      2009-08-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Otolith elemental fingerprints of Pacific herring Clupea apllasii in northern Pacific part of Japan2009

    • Author(s)
      Yamane K, Shirai K, Yamane S, Nagakura Y, Arai T
    • Organizer
      Fourth International Symposium on Fish Otolith Research and Application
    • Place of Presentation
      Monterey, USA
    • Year and Date
      2009-08-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] 耳石微量元素組成を指標とした北日本太平洋沿岸に生息するニシンの系群判別2009

    • Author(s)
      山根広大, 白井厚太朗, 山根幸伸, 長倉義智, 新井崇臣
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] ニシンの生活史に伴った耳石微量元素組成の変化2009

    • Author(s)
      山根広大, 白井厚太朗, 山根幸伸, 長倉義智, 新井崇臣
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report
  • [Presentation] 産卵回遊中のウナギAnguilla japonicaの回遊履歴と再生産への寄与2009

    • Author(s)
      茅野尚子, 新井崇臣
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2009-03-28
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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