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Molecular basis of interaction between virus and vector mosquito

Research Project

Project/Area Number 20688013
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

KANUKA Hirotaka  帯広畜産大学, 原虫病研究センター, 教授 (50342770)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2010: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2008: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000)
Keywords蚊 / ウイルス / RNA / 干渉 / 自然免疫 / 感染症 / 節足動物 / RNA干渉 / RNA干渉法
Research Abstract

Blood-sucking arthropods, including mosquitoes transmit numerous virus disease during blood feeding. Using a virus infection model in Drosophila and Aedes mosquito, we uncovered genetically encoded resistance of vector mosquito to virus infection as measured by the extent to which survival rate decreased via increasing virus burden. These results suggest that anti-virus responses of vector mosquito are not restricted to its survival but rather can be applied to capacity to employ virus mutualism during the disease transmission.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (43 results)

All 2011 2010 2009 2008 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (27 results) Remarks (1 results)

  • [Journal Article] A single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes2010

    • Author(s)
      Hiroka Aonuma, Aya Yoshimura, Tomomi Kobayashi, Kiyoshi Okado, Athanase Badolo, Bryce Nelson, Hirotaka Kanuka and Shinya Fukumoto
    • Journal Title

      Exp Parasitol

      Volume: 125(2) Pages: 179-183

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 感染に対する宿主側トレランス機構の発見2010

    • Author(s)
      新澤直明、嘉糠洋陸
    • Journal Title

      生化学

      Volume: 82(11) Pages: 1051-1055

    • NAID

      10027708729

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] ADF2 is required for transformation of the ookinete and sporozoite in malaria parasite development2010

    • Author(s)
      Doi Y, Shinzawa N, Fukumoto S, Okano H, Kanuka H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 397(4) Pages: 668-672

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] p38 MAPK-Dependent Phagocytic Encapsulation Confers Infection Tolerance in Drosophila2009

    • Author(s)
      Naoaki Shinzawa, Bryce Nelson, Hiroka Aonuma, Kiyoshi Okado, Shinya Fukumoto, Masayuki Miura and Hirotaka Kanuka
    • Journal Title

      Cell Host Microbe

      Volume: 6(3) Pages: 244-252

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors: Dirofilaria immitis as a study model2009

    • Author(s)
      Hiroka Aonuma, Aya Yoshimura, Namal Perera, Naoaki Shinzawa, Hironori Bando, Sugao Oshiro, Bryce Nelson, Shinya Fukumoto and Hirotaka Kanuka
    • Journal Title

      Parasit Vectors

      Volume: 2(1) Issue: 1

    • DOI

      10.1186/1756-3305-2-15

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid recruitment of innate immunity regulates variation of intracellular pathogen resistance in Drosophila2009

    • Author(s)
      Kiyoshi Okado, Naoaki Shinzawa, Hiroka Aonuma, Bryce Nelson, Shinya Fukumoto, Kozo Fujisaki, Shin-ichiro Kawazu and Hirotaka Kanuka
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 379(1) Pages: 6-10

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification2009

    • Author(s)
      Namal Perera, Hiroka Aonuma, Aya Yoshimura, Tokiyasu Teramoto, Hiroshi Iseki, Bryce Nelson, Ikuo Igarashi, Takeshi Yagi, Shinya Fukumoto and Hirotaka Kanuka
    • Journal Title

      J Virol Methods

      Volume: 156(1-2) Pages: 32-36

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 貪食性囲い込みは細菌感染に対するトレランスを制御する2009

    • Author(s)
      新澤直明、嘉糠洋陸
    • Journal Title

      細胞工学

      Volume: 28 Pages: 1278-1279

    • Related Report
      2010 Final Research Report
  • [Journal Article] ショウジョウバエを用いたヒト疾患分子基盤の解明-光るハエは医学へ貢献できるか2009

    • Author(s)
      新澤直明、嘉糠洋陸
    • Journal Title

      医学のあゆみ

      Volume: 230 Pages: 129-133

    • Related Report
      2010 Final Research Report
  • [Journal Article] Loop-mediated isothermal amplification applied to filarial parasites detection in the mosquito vectors : Dirofilaria immitis as a study model2009

    • Author(s)
      Aonuma, H., Yoshimura, A., Perera, N., Shinzawa, N., Bando, H., Oshiro, S, Nelson, B., Fukumoto, S., Kanuka, H.
    • Journal Title

      Parasites & Vectors 2

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] p38 MAPK-Dependent Phagocytic Encapsulation Confers Infection Tolerance in Drosophila2009

    • Author(s)
      Shinzawa, N., Nelson, B., Aonuma, H., Okado, K., Fukumoto, S., Miura, M., Kanuka, H.
    • Journal Title

      Cell Host & Microbe 6

      Pages: 244-252

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid recruitment of innate immunity regulates variation of intracellular pathogen resistance in Drosophila.2009

    • Author(s)
      Okado K, Shinzawa N, Aonuma H, Nelson B, Fukumoto S, Fujisaki K, Kawazu S, Kanuka H.
    • Journal Title

      Biochem Biophys Res Commun 379

      Pages: 6-10

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid identification of virus-carrying mosquitoes using reverse transcription-loop-mediated isothermal amplification.2009

    • Author(s)
      Perera N, Aonuma H, Yoshimura A, Teramoto T, Iseki H, Nelson B, Igarashi I, Yagi T, Fukumoto S, Kanuka H.
    • Journal Title

      J Virol Methods 156

      Pages: 32-36

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification2008

    • Author(s)
      Hiroka Aonuma, Moemi Suzuki, Hiroshi Iseki, Namal Perera, Bryce Nelson, Ikuo Igarashi, Takeshi Yagi, Hirotaka Kanuka and Shinya Fukumoto
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 376(4) Pages: 671-676

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid identification of Plasmodium-carrying mosquitoes using loop-mediated isothermal amplification.2008

    • Author(s)
      Aonuma H, Suzuki M, Iseki H, Perera N, Nelson B, Igarashi I, Yagi T, Kanuka H, Fukumoto S.
    • Journal Title

      Biochem Biophys Res Commun 376

      Pages: 671-676

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Odor-based bioenhanced transmission of pathogen by Drosophila melanogaster2011

    • Author(s)
      岡戸清、新澤直明、福本晋也、嘉糠洋陸
    • Organizer
      52nd Annual Drosophila Research Conference
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2011-03-31
    • Related Report
      2010 Final Research Report
  • [Presentation] トランスジェニック蚊を用いたウイルス生ワクチン産生の試み2010

    • Author(s)
      鹿島千紗子、新澤直明、高橋慧、福本晋也、下島昌幸、河岡義裕、嘉糠洋陸
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] ショウジョウバエは病原体を嗅覚で認識し摂食媒介する2010

    • Author(s)
      岡戸清、新澤直明、福本晋也、嘉糠洋陸
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 宿主-病原体相互作用における腸管内細菌の"ゆらぎ"2010

    • Author(s)
      伴戸寛徳、青沼宏佳、N'Fale Sagnon、福本晋也、嘉糠洋陸
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Midgut-based insect and parasite interaction in malaria vector Anopheles mosquitoes2010

    • Author(s)
      嘉糠洋陸
    • Organizer
      XIIth International Congress of Parasitology
    • Place of Presentation
      メルボルン
    • Year and Date
      2010-08-17
    • Related Report
      2010 Final Research Report
  • [Presentation] Midgut bacteria regulates Plasmodium development in malaria vector Anopheles mosquitoes2010

    • Author(s)
      伴戸寛徳、青沼宏佳、岡戸清、新澤直明、Moussa Guelbeogo、N'Fale Sagnon、福本晋也、嘉糠洋陸
    • Organizer
      Keystone Symposia "Molecular Targets for Control of Vector-Borne Diseases: Bridging Lab and Field Research"
    • Place of Presentation
      コロラド
    • Year and Date
      2010-04-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Midgut-based insect and parasite interaction in malaria vector Anopheles mosquitoes2010

    • Author(s)
      嘉糠洋陸
    • Organizer
      STINT workshop on Invertebrates
    • Place of Presentation
      スウェーデン
    • Year and Date
      2010-03-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Midgut-based insect and parasite interaction in malaria vector Anopheles mosquitoes2010

    • Author(s)
      嘉糠洋陸
    • Organizer
      STINT workshop on Invertebrates
    • Place of Presentation
      スウェーデン国ウプサラ市
    • Year and Date
      2010-03-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] マラリア媒介蚊における腸内細菌と病原体のインターフェース2009

    • Author(s)
      嘉糠洋陸
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 機械的な病原体伝播と自然免疫の相互作用2009

    • Author(s)
      岡戸清、新澤直明、福本晋也、嘉糠洋陸
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] トランスジェニック蚊を用いたウイルス生ワクチン産生の試み2009

    • Author(s)
      鹿島千紗子、新澤直明、高橋慧、福本晋也、下島昌幸、河岡義裕、嘉糠洋陸
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] ハエ不顕性感染モデル~貪食性囲い込みは細菌感染に対するトレランス機能を制御する~2009

    • Author(s)
      新澤直明、Bryce Nelson、青沼宏佳、岡戸清、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] ベクターバイオロジー:モデル生物による新展開2009

    • Author(s)
      嘉糠洋陸
    • Organizer
      平成21年度国立大学附置研究所・センター長会議第2部会シンポジウム
    • Place of Presentation
      帯広
    • Year and Date
      2009-09-19
    • Related Report
      2010 Final Research Report
  • [Presentation] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila2009

    • Author(s)
      新澤直明、Bryce Nelson、青沼宏佳、岡戸清、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      9th Awaji International Forum of Infection and Immunity
    • Place of Presentation
      兵庫
    • Year and Date
      2009-09-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Serratia marcescens regulates Plasmodium development in mosquito midgut2009

    • Author(s)
      伴戸寛徳、青沼宏佳、岡戸清、新澤直明、Guelbeogo Moussa, N'Fale Sagnon、福本晋也、嘉糠洋陸
    • Organizer
      EMBO WORKSHOP "Molecular and Population Biology of Mosquitoes and Other Disease Vectors
    • Place of Presentation
      ギリシャ
    • Year and Date
      2009-07-22
    • Related Report
      2010 Final Research Report
  • [Presentation] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila2009

    • Author(s)
      新澤直明、青沼宏佳、岡戸清、Bryce Nelson、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      第9回ショウジョウバエ研究会
    • Place of Presentation
      静岡
    • Year and Date
      2009-07-06
    • Related Report
      2010 Final Research Report
  • [Presentation] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila2009

    • Author(s)
      新澤直明、青沼宏佳、岡戸清、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      Jacques Monod Conference "Insect immunity in action: from fundamental mechanisms of host defense to resistance against infections in nature"
    • Place of Presentation
      フランス
    • Year and Date
      2009-05-25
    • Related Report
      2010 Final Research Report
  • [Presentation] 貪食細胞による囲い込みは細菌感染に対するtolerance機能を宿主に付与する2008

    • Author(s)
      新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 蚊・マラリア原虫・腸内細菌の三者の相互作用2008

    • Author(s)
      伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] 貪食細胞による囲い込みは細菌感染に対するtolerance機能を宿主に付与する2008

    • Author(s)
      新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸(兵庫)
    • Year and Date
      2008-12-10
    • Related Report
      2008 Annual Research Report
  • [Presentation] p38 MAPK-dependent phagocytic encapsulation confers infection tolerance in Drosophila2008

    • Author(s)
      嘉糠洋陸
    • Organizer
      Swedish-Japan STINT-meeting on Innate Immunity
    • Place of Presentation
      福岡
    • Year and Date
      2008-10-16
    • Related Report
      2010 Final Research Report
  • [Presentation] 感染現象とそれと対峙する宿主の抵抗戦略2008

    • Author(s)
      嘉糠洋陸
    • Organizer
      第8回昆虫病理研究会シンポジウム
    • Place of Presentation
      山梨
    • Year and Date
      2008-09-11
    • Related Report
      2010 Final Research Report
  • [Presentation] Cellular encapsulation-based host tolerance in bacterial infection2008

    • Author(s)
      新澤直明、青沼宏佳、福本晋也、三浦正幸、嘉糠洋陸
    • Organizer
      8th Awaji International Forum of Infection and Immunity
    • Place of Presentation
      兵庫
    • Year and Date
      2008-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Impact of midgut bacteria on Plasmodium development in mosquito2008

    • Author(s)
      伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
    • Organizer
      8th Awaji International Forum of Infection and Immunity
    • Place of Presentation
      兵庫
    • Year and Date
      2008-09-10
    • Related Report
      2010 Final Research Report
  • [Presentation] ウイルス媒介蚊における抗ウイルス反応メカニズムの解析2008

    • Author(s)
      寺本時靖、吉村文、福本晋也、嘉糠洋陸
    • Organizer
      日本分子生物学会第8回春季シンポジウム
    • Place of Presentation
      札幌
    • Year and Date
      2008-05-26
    • Related Report
      2010 Final Research Report
  • [Presentation] Innate immune response activity correlates to diversity of Drosophila susceptibility to bacterial infection2008

    • Author(s)
      岡戸清、新澤直明、青沼宏佳、福本晋也、嘉糠洋陸
    • Organizer
      49th Annual Drosophila Research Conference
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2008-04-04
    • Related Report
      2010 Final Research Report
  • [Presentation] ハマダラカ中腸に存在する細菌がマラリア原虫の発育に与える影響2008

    • Author(s)
      伴戸寛徳、青沼宏佳、福本晋也、嘉糠洋陸
    • Organizer
      第77回日本寄生虫学会
    • Place of Presentation
      長崎
    • Year and Date
      2008-04-02
    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://jikei-tropmed.jp/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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